Purification and the antioxidant properties of saponins in soy molasses
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摘要: 为从大豆糖蜜中提取和纯化大豆皂苷,本研究通过单因素优化提取条件,探究AB-8树脂对大豆皂苷的吸附性能,利用乙醇的梯度洗脱对皂苷进行纯化;并通过测定大豆皂苷的羟自由基抑制能力、超氧自由基清除能力和总抗氧化能力对其抗氧化性质进行研究。结果表明:乙醇浓度为75%、料液比为1∶25、在70℃下提取4 h,大豆皂苷的得率最高,为（167.81±6.19）mg/g,此时皂苷纯度为42.57%±2.34%;AB-8树脂对皂苷的吸附率和解吸率分别为68.50%和49.99%;柱层析上样温度为25℃,皂苷提取液经AB-8树脂吸附和099%乙醇的梯度洗脱后,皂苷纯度提高到73.21%±1.05%;皂苷浓度在0.51.5 mg/m L内,其羟自由基抑制能力和总抗氧化能力随浓度的增加而提高,最高为（47.25±0.34）U/mg和（0.82±0.04）mmol/g,在2.04.0 mg/m L内,其抗超氧阴离子自由基能力随浓度增加而提高,最高为（28.93±1.07）U/g。本研究对于大豆糖蜜的利用及皂苷提纯具有参考价值。Abstract: Aiming to purify saponins from soybean molasses, single-factor experiments was carried out to optimize the extraction. The adsorption properties of AB-8 resin on saponins was studied, and the gradient elution was used to purify the saponins.The antioxidant properties of saponins were studied by the hydroxyl radical inhibition ability, superoxide radical scavenging capacity, and total antioxidant capacity.The optimized condition determined was that ethanol concentration of 75%, ratio of ethanol/water to raw material 25 ∶ 1, at 70 ℃ for 4 h, and the actual yield of saponins was ( 167.81 ± 6.19) mg/g.Meanwhile, the purity of saponins was 42.57% ± 2.34%. The adsorption rate and desorption rate of AB-8 resin on saponins were 68.50% and 49.99%, respectively.The temperature was 25 ℃ of column chromatography.Moreover, the purity of saponins reached to 73.21% by AB-8 resin column chromatography and 0 ~ 99% ethanol gradient elution. Among the saponins concentration of 0.5 ~ 1.5 mg/m L, the hydroxyl radical inhibition ability and total antioxidant capacity increased with the increasing concentration, the maximum was ( 47.25 ± 0.34) U/mg and ( 0.82 ± 0.04) mmol/g, respectively.Among 2.0~4.0 mg/m L, the superoxide radical scavenging capacity increased with the increase of concentration, the maximum was ( 28.93 ± 1.07) U/g.The study is valuable to the comprehensive utilization of soybean molasses and the purification of saponins.
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