蓝靛果多酚冻干粉稳定性及其总抗氧化能力研究

雷月; 宫彦龙; 朱速松; 李斌

doi:10.13386/j.issn1002-0306.2017.13.015

蓝靛果多酚冻干粉稳定性及其总抗氧化能力研究

1. 贵州省水稻研究所
2. 沈阳农业大学食品学院

基金项目:

国家自然科学基金资助(31671863)；辽宁省高等学校优秀人才支持计划资助(2014108)；沈阳农业大学天柱山英才计划项目资助(2014)；贵州省优秀青年科技人才培养对象专项资金资助(黔科合人字[2011]19号)；贵州省科技计划资助(黔科合重大专项字[2013]6023号)；贵州省科研机构服务企业行动计划资助(黔科合服企[2014]4005)；贵州省科技合作计划资助(黔科合LH字[2015]7074号)；贵州省现代水稻产业技术体系资助(GZCYTX2016-06)；

详细信息

作者简介:
雷月 (1988-) , 女, 硕士, 研究实习员, 研究方向:食品深加工, E-mail:leiyue0917@163.com。;

李斌 (1979-) , 男, 博士, 副教授, 研究方向:浆果深加工及功能性成分研究, E-mail:libinsyau@163.com。;

中图分类号: TS255.1
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出版历程
- 收稿日期: 2016-12-26

Study on stability and total antioxidant capacity of polyphenols from Lonicera Caerulea L.

Guizhou Rice Research Institute
College of Food Science, Shenyang Agricultural University

摘要

摘要: 对采自长白山的蓝靛果忍冬冻果经提取、纯化,并制成蓝靛果多酚冻干粉,研究p H、光照、温度、氧化剂H2O2、还原剂Na₂SO3、防腐剂苯甲酸钠和葡萄糖、蔗糖、D-果糖对蓝靛果多酚冻干粉稳定性的影响,并通过总抗氧化能力（Total Antioxidant Capacity,T-AOC）测试,进一步分析了蓝靛果多酚冻干粉在不同条件下的抗氧化活性。结果表明:蓝靛果多酚在p H为2和3、低温避光条件下保存效果较好,蓝靛果多酚耐还原能力比耐氧化能力强,防腐剂苯甲酸钠对蓝靛果多酚稳定性无显著影响（p>0.05）,糖的加入有助于多酚保存率的提高,使其稳定性增强;通过总抗氧化能力（T-AOC）测试发现,在p H为2、3时,蓝靛果多酚的总抗氧化能力明显高于其他受试p H范围（p<0.05）,温度、光照对蓝靛果多酚抗氧化活性的影响存在显著性差异（p<0.05）,添加氧化剂H2O2会降低多酚的抗氧化活性,添加不同质量浓度的糖类对蓝靛果多酚抗氧化活性的影响显著（p<0.05）。
- 蓝靛果 /
- 多酚 /
- 稳定性 /
- 总抗氧化能力
Abstract: In this study, the polyphenols from Lonicera Caerulea L was obtained through the extraction, purification and vacuum freeze drying, and the effect of p H, light, temperature, H2O2, Na2SO3, sodium benzoate and glucose, sucrose, D fructose on the stability of polyphenols were investigated.Through determining the total antioxidant capacity ( T-AOC) , the antioxidant activity of polyphenols from Lonicera Caerulea L. in different conditions was analyzed. The results showed that the polyphenols from Lonicera Caerulea L. had better preservation when the p H was 2 and 3, at low temperature and in dark, the resistance to reduction of polyphenols was stronger than ability of resistance to oxidation, sodium benzoate had no obvious impact on the stability of polyphenols from Lonicera Caerulea L. ( p > 0.05) , carbohydrate increased the stability of polyphenols. In addition, through determination of the total antioxidant capacity, it was found that the total antioxidant capacity of polyphenols was obviously higher than that of other subjects p H range when the p H was 2 and 3 ( p < 0.05) , the temperature and light had obvious impact on the antioxidant activity of polyphenols from Lonicera Caerulea L. ( p < 0.05) , H2O2 reduced the antioxidant activity of polyphenols, the carbohydrate at different concentrations had obvious effects on the antioxidant activity of polyphenols ( p < 0.05) .
- Lonicera Caerulea L /
- polyphenols /
- stability /
- total antioxidant capacity

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参考文献(35)

[1]	吕闻明.蓝靛果忍冬酚类物质提取及抗氧化性研究[D].长春:吉林大学, 2012.
[2]	刘敬华, 王振宇.精制及高纯度蓝靛果花色苷的抗氧化性及稳定性研究[J].食品工业科技, 2013, 34 (19) :87-92.
[3]	Sun Feng, Huo Junwei, QIN Dong.Establishment and optimization of SRAP amlification system in Lonicera Caerulea L[J].Journal of Northeast Agricultural University (English Edition) , 2011, 18 (4) :26-31.
[4]	Jin X H, Ohgami K, Shiratori K, et al.Effects of blue honeysuckle (Lonicera caerulea L.) extract on lipopolysaccharideinduced inflammation in vitro and in vivo[J].Experimental Eye Research, 2006, 82 (5) :860-867.
[5]	刘德江, 申健, 田立娟, 等.野生与栽培蓝靛果果实提取物的体外抗氧化活性[J].经济林研究, 2013, 31 (3) :103-106.
[6]	张敏, 刘刚, 张雁南, 等.响应面法优化蓝靛果花色苷提取工艺研究[J].北方园艺, 2014 (23) :113-116.
[7]	向延菊, 王大伟.蓝靛果忍冬的研究现状及其发展前[J].塔里木农垦大学学报, 2004, 16 (4) :26-29.
[8]	Kahkonen M P, Hopia A I, Heinonen M.Berry phenolics and their antioxidant activity[J].Journal of Agricultural and Food Chemistry, 2001, 49 (8) :4076-4082.
[9]	Gruia MI, Oprea, et al.The antioxidant response induced by Lonicera caerulaea berry extracts in animals bearing experimental solid tumors[J].Molecules, 2008 (13) :1195-1206.
[10]	Kim J H, Hong J Y, Shin S R, et al.Comparison of antioxidant activity in wild plant (Adenophora triphylla) leaves and roots as a potential source of functional foods[J].International Journal of Food Sciences and Nutrition, 2009 (60) :150-161.
[11]	R Puupponen-Pimi, L Nohynek, C Meier, et al.Antimicrobial properties of phenolic compounds from berries[J].Journal of Applied Microbiology, 2001, 90 (4) :494-507.
[12]	Zhu X, Zhang H, Lo R, et al.Phenolic compounds from the leaf extract of artichoke (Cynara scolymus L.) and their antimicrobial activities[J].Journal of Agricultural and Food Chemistry, 2004 (52) :7272-7278.
[13]	Park S, Hahm KB, Oh TY, et al.Preventive effect of the flavonoid, wogonin, against ethanol-induced gastric mucosal damage in rats[J].Dig Dis Sci, 2004 (49) :384-394.
[14]	Fawole O A, Ndhlala A R, Amoo S O, et al.Antiinflammatory and phytochemical properties of twelve medicinal plants used for treating gastro-intestinal ailments in South Africa[J].Journal of Ethnopharmacology, 2009, 123 (2) :237-43.
[15]	Liang C, Xin X, Rong L, et al.Isolation of cyanidin3-glucoside from blue honeysuckle fruits by high-speed countercurrent chromatography[J].Food Chemistry, 2014, 152 (2) :386-390.
[16]	Chen L, Xin X, Yuan Q, et al.Phytochemical properties and antioxidant capacities of various colored berries[J].Journal of the Science of Food and Agriculture, 2014, 94 (2) :180-188.
[17]	Fukumoto L R, Mazza G.Assessing antioxidant and prooxidant activities of phenolic compounds[J].Journal of Agricultural and Food Chemistry, 2000, 48 (8) :3597-3604.
[18]	Augustine S, Kudachikar V B, Vanajakshi V, et al.Effect of combined preservation techniques on the stability and microbial quality and retention of anthocyanins in grape pomace stored at low temperature[J].Journal of Food Science and Technology, 2013, 50 (2) :332-338.
[19]	Daniela F, Cristian D B, Paolo S, et al.Effect of Time and storage temperature on anthocyanin decay and antioxidant activity in wild blueberry (Vaccinium angustifolium) powder[J].Journal of Agricultural and Food Chemistry, 2013, 61 (12) :2999-3005.
[20]	张冬雪.蓝靛果色素提取条件对比, 稳定性及应用研究[D].北京:中国农业科学院, 2012.
[21]	冯卫华, 于立梅, 秦艳, 等.荔枝多酚的抗氧化性动力学及稳定性[J].食品科学, 2011, 32 (15) :5-9.
[22]	袁歆贻.p H与光照对苹果多酚稳定性的影响[J].现代农业科技, 2014 (19) :297-304.
[23]	赵玉, 任亚梅, 张爽, 等.苹果果皮和果肉多酚稳定性研究[J].中国食品学报, 2014, 14 (2) :93-101.
[24]	赵玉, 任亚梅, 李莹, 等.添加剂对苹果果皮和果肉多酚稳定性的影响[J].中国食品学报, 2015, 15 (3) :120-127.
[25]	李斌, 雷月, 孟宪军, 等.响应面实验优化超声波辅助提取蓝靛果多酚工艺及其抗氧化活性[J].食品科学, 2015, 36 (22) :33-39.
[26]	李斌, 雷月, 智红涛, 等.一种纯化蓝靛果忍冬中多酚的方法:中国, CN 105616477 A[P].2016.06.01.
[27]	楠极, 栗丽萍.番石榴叶多酚提取液稳定性的研究[J].食品研究与开发, 2016, 37 (8) :32-35.
[28]	王卫国, 林强, 张仟伟.葡萄皮花色苷的生理活性及稳定性研究进展[J].中国酿造, 2016, 35 (9) :5-10.
[29]	李伟, 张应团.火棘多酚类物质的体外抗氧化作用[J].食品工业科技, 2008 (9) :121-123.
[30]	毛迪锐, 姜贵全, 孙继伟, 等.文冠果壳总黄酮分离纯化及稳定性研究[J].食品与机械, 2016, 32 (1) :140-143.
[31]	高凝轩, 李斌, 杜珊珊, 等.黑果腺肋花楸多酚稳定性的研究[J].食品科学, 2016, 37 (23) :20-24.
[32]	樊金玲, 朱文学, 巩卫东, 等.蔗糖含量对牡丹花色苷热稳定性性和降解动力学的影响[J].食品科学, 2010, 31 (13) :74-78.
[33]	雷月, 黎盛, 智红涛, 等.粗制及精制蓝靛果花色苷的稳定性及抗氧化性研究[J].食品工业科技, 2016, 37 (2) :113-124.
[34]	周坦洋, 罗芙蓉, 白彬.葡萄籽原花青素生物药理活性的研究进展[J].哈尔滨医科大学学报, 2012, 46 (1) :94-96.
[35]	Lopes L B, Vande H, Li H T, et al.Topical delivery of lycopene using microemulsions:enhanced skin penetration and tissue antioxidant activity[J].Journal of Pharmaceutical Sciences, 2010, 99 (3) :1346-1357.