接骨草总黄酮提取工艺及其抑菌作用的研究

黄国文; 管天球; 赵雨云; 陈莫林; 刘宏辉

doi:10.13386/j.issn1002-0306.2017.13.007

接骨草总黄酮提取工艺及其抑菌作用的研究

湖南科技学院生化系

基金项目:

湖南省科技计划项目(2014NK2021)；湘南优势植物资源综合利用湖南省重点实验室资助项目(XNZW15C19)；

详细信息

作者简介:
黄国文 (1965-) , 男, 博士, 讲师, 从事植物生物学的教学和研究工作, E-mail:huanggwdax@163.com。;

中图分类号: TQ464
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出版历程
- 收稿日期: 2016-12-28

Study on the extraction and antibacterial effect of total flavonoids from Sambucus chinensis Lindl

Department of Biological Sciences and Chemical Engineerings, Hunan College of Science and Technology

摘要

摘要: 以碱溶酸沉法研究接骨草总黄酮的提取条件和抑菌作用。以料液比、温度、时间、提取液p H为影响因素,进行单因素实验和正交实验,研究提取接骨草总黄酮的最优条件,调节提取液p H来沉淀总黄酮,并用比浊法和滤纸圆片法研究总黄酮的抑菌作用。结果表明,提取接骨草总黄酮的最优条件是料液比1∶40(g/m L)、温度为80℃、时间为45 min、提取液p H10,此条件下接骨草总黄酮的得率为3.11%。调节总黄酮提取液p H3.5沉淀接骨草总黄酮的量较多,其得率为2.15%。接骨草总黄酮在酸性p H4~5时对金黄色葡萄球菌、枯草芽孢杆菌和大肠杆菌的抑制作用较强,并能够增加三种细菌的电导率,对三种菌最小抑菌浓度分别为0.63、1.25、2.50 mg/m L。用碱溶酸沉法提取接骨草总黄酮的工艺简单,总黄酮具有较高的抑菌活性,为进一步开发接骨草的总黄酮打下了基础。
- 接骨草 /
- 总黄酮 /
- 提取工艺 /
- 抑菌作用
Abstract: By using alkali soluble and acid sinking method, total flavonoids of Sambucus chinensis Lindl were extracted, and its antibacterial effect were studied in this paper. The extraction conditions including solid-liquid ratio, extraction temperature, extraction time, p H of xetraction liquid of flavonoids from Sambucus chinensis Lindl were optimized by single factor experiments and orthogonal experiments, and meanwhile the sinking efficiency of total flavonoids were studied by acidic p H precipitation, and the antibacterial activity of total flavonoids was studied by filter paper discs method and turbidimetric method. The results showed that the optimum extraction conditions of total flavonoids of Sambucus chinensis Lindl were as follow: solid-liquid ratio1∶ 40 ( g/m L) , extraction temperature 80 ℃, extraction time 45 min, extraction liquid p H10, in which the extraction rate of total flavonoids of Sambucus chinensis Lindl was 3.11%.Adapting the extract to p H3.5 with HCl could better precipitate about 2.15%total flavonoids of Sambucus chinensis Lindl.Total flavonoids of Sambucus chinensis Lindl had a stronger antibacterial activity on Staphylococcus aureus, Bacillus subtilis, Escherichia coli at acidic p H4 ~5 than at neutral p H and alkaline p H value, and these bacteria conductivity was increased by total flavonoids. The minimum inhibitory concentration of total flavonoids of Sambucus chinensis Lindl on three bacteria were 0.63, 1.25, 2.50 mg/m L, respectively. The extracting process of total flavonoids from Sambucus chinensis Lindl by using alkali soluble and acid sinking method was simple and inexpensive, and its total flavonoids had a great antibacterial activity, in which played a foundation on the development and utilization of total flavonoids of Sambucus chinensis Lindl.
- Sambucus chinensis Lindl /
- flavonoids /
- extraction process /
- antibacterial effect

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参考文献(29)

[1]	马养民, 吴昊.接骨木属植物的化学成分研究进展[J].有机化学, 2012, 32:2063-2072.
[2]	方建新.接骨草的开发利用[J].中国林副特产, 2007 (6) :85-86.
[3]	姚元枝, 伍贤进, 黎晓英, 等.接骨草的化学成分与药理活性研究进展[J].中成药, 2015, 37 (12) :2726-2732.
[4]	王长远, 吴洪奎, 于长青, 等.黄酮类化合物研究进展[J].黑龙江八一农垦大学学报, 2007, 19 (2) :75-78.
[5]	朱文振, 马龙, 李国荣.黄酮类化合物的抗癌作用及作用机制[J].生命科学, 2012, 24 (5) :444-449.
[6]	何佳珂, 于洋, 陈西敬, 等.黄酮类化合物的药物代谢研究进展[J].中国中药杂志, 2010, 35 (21) :2789-2794.
[7]	胡彦武, 刘凯, 闫梦彤, 等.淫羊藿总黄酮及淫羊藿苷的心血管保护作用及机制研究进展[J].中国实验方剂学杂志, 2015 (13) :227-230.
[8]	Wang LJ, Yang XS, Qin PY, et al.Flavonoid composition, antibacterial and antioxidant properties of tartary buckwheat bran extract[J].Industrial Crops and Products, 2013, 49:312-317.
[9]	邓红梅, 马超.皇帝柑皮中粗黄酮的提取及其抑菌活性研究[J].食品工业, 2013 (1) :21-24.
[10]	柯春林, 任茂生, 王娣, 等.黄酮化合物抗菌机理的研究进展[J].食品工业科技, 2015, 36 (2) :388-391.
[11]	魏良柱, 李筱玲.黄酮类化合物药理活性研究进展[J].陕西农业科学, 2014, 60 (12) :52-56.
[12]	Kumar S, Pandey AK.Chemistry and biological activities of flavonoids:an overview[J].Science World Journal, 2013.doi: 10.1155/2013/162750.
[13]	耿敬章, 冯君琪.黄酮类化合物的生理功能与应用研究[J].中国食物与营养, 2007 (7) :62-65.
[14]	熊燕, 姚元枝, 安晓刚, 等.接骨草药茶的炮制及黄酮类化合物含量的比较[J].中国民族医药杂志, 2013 (9) :53-55.
[15]	李伟.接骨草黄酮类物质超声提取工艺优化及其抗氧化活性[J].中药材, 2011, 34 (11) :1789-1791.
[16]	刘宁, 朱沛沛, 李嘉乐.黄酮类化合物提取工艺研究进展[J].饮料工业, 2012, 15 (7) :7-9.
[17]	Pan GY, Yu GY, Zhu CH, et al.Optimization of ultrasoundassisted extraction (UAE) of flavonoids compounds (FC) from hawthorn seed (HS) [J].Ultrasonics Sonochemistry, 2012, 19:486-490.
[18]	蔡凌云.接骨草黄酮成分的初步研究[J].凯里学院学报, 2010, 28 (6) :62-64.
[19]	陈继光, 米丽雪, 上官新晨, 等.青钱柳愈伤组织总黄酮碱溶酸沉提取工艺[J].食品科学, 2011, 32 (16) :103-107.
[20]	田哲榕, 侯先槐.干豌豆中皂苷与黄酮类化合物含量研究[J].忻州师范学院学报, 2011, 27 (2) :128-130.
[21]	Li XN, Huang JL, Wang ZD, et al.Alkaline extraction and acid precipitation of phenolic compounds from longan (Dimocarpus longan L.) seeds[J].Separation and Purification Technology, 2014, 124:201-205.
[22]	赵洪, 杨颖, 黎李.碱溶液提取竹叶总黄酮工艺的初步研究[J].山东化工, 2015, 44:58-59, 62.
[23]	唐红军.中国药典2000年版一部检测芦丁含量方法的改进[J].中国医院药学杂志, 2002, 22 (1) :60.
[24]	赵文红, 范青生, 马永花, 等.杜仲叶中总黄酮的醇提工艺[J].南昌大学学报 (理科版) , 2007, 31 (1) :69-72.
[25]	张雁, 池建伟, 张名位, 等.新鲜葛根中总黄酮和淀粉的提取工艺研究[J].中国食品学报, 2008, 8 (3) :73-77.
[26]	盛玉青, 侍海娇, 邓晓亚, 等.大孔吸附树脂纯化凤尾草总黄酮工艺研究[J].现代中药研究与实践, 2014, 28 (6) :52-55.
[27]	郑津辉, 王威, 黄辉.苦参提取液中黄酮类化合物的抑菌作用[J].武汉大学学报 (理学版) , 2008, 54 (4) :439-442.
[28]	许明, 韩亮, 李艳琴, 等.苦荞黄酮类化合物的稳定性研究[J].食品与药品, 2012 (9) :305-308.
[29]	陈国妮, 孙飞龙, 闫亚茹, 等.马齿苋黄酮类化合物抑菌机理的研究[J].化学与生物工程, 2015, 32 (10) :34-37.

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