响应面法优化灵芝孢子粉中灵芝酸的破壁提取工艺

冯印; 刘乔; 李岩; 刘艳; 沈明浩

doi:10.13386/j.issn1002-0306.2017.12.035

响应面法优化灵芝孢子粉中灵芝酸的破壁提取工艺

长春科技学院生物食品学院

详细信息

作者简介:
冯印 (1986-) , 女, 硕士, 研究方向:食品微生物与功能性食品, E-mail:113889523@qq.com。;

沈明浩 (1963-) , 男, 博士, 教授, 研究方向:食品毒理与安全, 胚胎毒理, E-mail:shenmh2003@163.com。;

中图分类号: TQ464
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出版历程
- 收稿日期: 2016-12-11

Optimization of wall-breaking extraction technology of Ganoderic acid from Ganoderma Spores ( GS) using response surface methodology

College of Biological and Food, Changchun Science and Technology University

摘要

摘要: 目的:以超声提取法为主,漆酶破壁法为辅助提取灵芝孢子粉中的灵芝酸,对影响提取得率的几个因素进行优化。方法:借助Design-Expert软件,利用Placket-Burman实验从超声时间、超声次数、溶剂、料液比、酶量、酶解时间、酶解温度和p H这8个影响提取得率的因素中筛选出四个主要因素,分别为超声时间、超声次数、溶剂和酶解时间,在此基础上,用Box-Behnken实验设计及响应面分析法进行回归分析。结果:当超声时间为3.2 h,溶剂用97%的乙醇,超声次数2次,酶解时间1.2 h时,提取得率达到最高,为3.40%,与初始提取得率2.4%相比,提取得率提高了1.4倍。结论:采用漆酶破壁与超声提取方法较大幅度提高灵芝酸的提取得率,并缩短了提取时间。
- 漆酶 /
- 灵芝孢子粉 /
- 灵芝酸
Abstract: Objective: Ganoderma Spores (GS) was used as raw material as the ultrasonic extraction as the main method and laccase wall-breaking method the secondary one to extract Ganoderic acid, the extraction factors affected the extraction ratio were optimized.Methods: Ultrasonic time, ultrasonic frequency, solvent and enzymolysis time were identified as the main factors from asonic time, ultrasonic frequency, solvent, solid-liquid ratio, enzyme amount, enzymolysis time, digestion temperature and p H which influenced the extraction ratio using experimental design of Placket-Burman (PB) by design-expert software.On this basis, the experimental design of Box-Behnken (BBD) and response surface analysis method was used for regression analysis.Results: When the ultrasonic time was 3.2 h, the solvent was 97% ethanol, ultrasonic frequency of 2 times, enzymolysis time for1.2 h, reached the highest extraction rate was 3.40%, and the extraction yield increased 1.4 times compared with the initial design.The extraction rate of ganoderma lucidum acid was increased greatly and the extraction time was shorten by the combined ues of cell wall disruption with lasscase and ultrasonic extraction.
- laccase /
- GS /
- Ganoderic acid

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参考文献(15)

[1]	Kubota T, Asaka Y, Miura I, et al.Structures of Ganoderic Acid A and B, Two New Lanostane Type Bitter Triterpenes from Ganoderma lucidum (FR.) KARST[J].Helvetica Chimica Acta, 1982, 65 (2) :611-619.
[2]	中华人民共和国卫生部药典委员会.中华人民共和国药典[M].一部.北京:化学工业出版社, 2000:137.
[3]	曾祥丽, 包海鹰.灵芝三萜类成分与药理学研究进展[J].菌物研究, 2004, 2 (1) :68-77.
[4]	罗俊, 林志彬.灵芝三萜化合物药理作用研究发展[J].药学学报, 2002, 37 (7) :42-46.
[5]	El-Mekkawy S, Meselhy, Nakamura N, et al.Anti-HIV-1 and anti-HIV-1-protease from Ganoder maluciduma[J].Phytochemistry.1998, 49 (5) :1651-1657.
[6]	王斌, 胡岳山, 李杰芬.灵芝三萜对小鼠脾脏树突状细胞增殖的影响[J].中药材, 2005, 28 (7) :21-25.
[7]	周昌艳, 唐庆九, 杨焱, 等.灵芝中有效成分灵芝酸的抑制肿瘤作用研究[J].茵物学报, 2004, 23 (2) :275-279.
[8]	H H, C M, M R, et al.Effect of 26-oxygenosterols from Ganoderma lucidum and their activity as cholesterol synthesis inhibitors.[J].Appl Environ Microbiol, 2005, 71 (7) :3653-3658.
[9]	SB L, CH L, SS L, et al.Triterpene-Enriched Extracts From Ganoderma Lucidum Inhibit Growth Of Hepatoma Cells Via Suppressing Protein Kinase C, Activating Mitogen-Activated Protein Kinases And G2-Phase Cell Cycle Arrest[J].Life Sciences, 2003, 72 (21) :2381-2390.
[10]	姚雪坤, 张玥, 罗俊, 等.灵芝三萜类物质对环磷酰胺免疫抑制模型小鼠的免疫调节作用[A].2011国际灵芝研究学术会议文集[C].2011.
[11]	马晶晶.高速离心剪切粉碎对灵芝孢子粉破壁及特性的影响[D].武汉:武汉理工大学, 2006.
[12]	朱江, 梅跃明, 李玉龙, 等.灵芝孢子粉的生产工艺研究[J].江西食品工业, 2006 (4) :29-29.
[13]	李承范.野生与种植灵芝中灵芝酸和总黄酮的提取及其含量测定[J].延边大学学报:自然科学版, 2013, 39 (4) :269-272.
[14]	杨义芳.中药提取分离新技术[M].北京:化学工业出版社, 2010.
[15]	杨林, 林鹏, 钱娇玲, 等.响应面法优化锁阳总黄酮超声提取工艺[J].中医药学报, 2013, 41 (5) :76-80.