Abstract: To provide the base for distinguishing Citrus medica L. var. sarcodactylis Swingle from different regions and quality evaluation, the ~1H-NMR fingerprint analysis of C.medica L.var.sarcodactylis Swingle was established.The samples collected from diverse geographical locations were determined by the ~1H-NMR method. The NMR data was evaluated by the methods of similarity analysis, cluster analysis and principal component analysis. The experimental results showed that the similarity value was above 0.95 between C. medica L. var. sarcodactylis Swingle from Jinhua and C. medica L. var. sarcodactylis Swingle from Sichuan.However, the similarity value was with under 0.86 between C. medica L. var. sarcodactylis Swingle from Jinhua and C.medica L. var. sarcodactylis Swingle from Guangdong. The relative component of limettin in C. medica L. var. sarcodactylis Swingle from Jinhua and Sichuan were greater than that of C.medica L.Var.sarcodactylis Swingle from Guangdong. The result of cluster analysis also showed C. medica L. var. sarcodactylis Swingle from Guangdong was different from the C. medica L. var.sarcodactylis Swingle from Jinhua and Sichuan, but the C. medica L. var. sarcodactylis Swingle from Jinhua was similar to the C.medica L.var.sarcodactylis Swingle from Sichuan. According to the principal components analysis, four principal components was obtained, and the contribution rate of accumulative total of variance of the four factors was 95.352%.The established method was a quick, simple and useful for distinguishing different species and assessment of the quality of the C.medica L.var.sarcodactylis Swingle.