响应面法优化多汁乳菇多糖提取工艺及抗氧化活性研究

黄娟; 黄燕燕; 刘冬梅; 陈素芹; 潘伟才

doi:10.13386/j.issn1002-0306.2017.11.002

响应面法优化多汁乳菇多糖提取工艺及抗氧化活性研究

华南理工大学食品科学与工程学院
深圳市大百汇技术有限公司

基金项目:

深圳市未来产业专项资金项目(CXZZ20140902155219302)；国家自然科学基金项目(31101254)；广东省科技计划项目(2014A020208019)；

详细信息

作者简介:
黄娟 (1991-) , 女, 硕士, 研究方向:食品微生物利用与控制, E-mail:juanhuangscut@126.com。;

刘冬梅 (1972-) , 女, 博士, 副教授, 主要从事食品微生物利用与控制研究, E-mail:liudm@scut.edu.cn。;

中图分类号: TQ464.1
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出版历程
- 收稿日期: 2016-12-28

Optimization of extraction process of polysaccharides from wild Lactarius volemus by response surface method and investigation of antioxidant activity

Institute of Food Science and Engineering, South China University of Technology
Da Baihui Company of Technology

摘要

摘要: 本文意在优化多汁乳菇多糖的提取工艺,探讨多汁乳菇多糖抗氧化活性。本文以浸提温度、浸提时间、液料比为考察因素,在单因素实验的基础上,设计响应面法Central-Composite中心组合实验,对多汁乳菇多糖提取工艺参数进行优化,同时,通过O₂^-·、·OH、DPPH及ABTS自由基清除实验探究了多汁乳菇多糖的抗氧化活性。结果表明,多汁乳菇多糖的最佳提取工艺:浸提温度90℃,浸提时间4 h,液料比33∶1 m L/g,对应多糖得率为2.18%,产品中多糖纯度为55.40%,蛋白含量为11.37%,不含淀粉;多汁乳菇多糖对O₂^-·、·OH、DPPH及ABTS自由基清除活性的IC50值分别为:868.16、280.00、342.06、167.65μg/m L。可见,响应面法可有效拟合多汁乳菇多糖得率与浸提温度、浸提时间、液料比之间的关系,且多汁乳菇多糖具有较高的抗氧化活性。
- 多汁乳菇 /
- 多糖 /
- 提取 /
- 响应面 /
- 抗氧化活性
Abstract: In order to study the extraction process of wild Lactarius volemus polysaccharide ( LVP) and its antioxidant activity, Central-Composite Design ( CCD) of response surface design ( RSD) was used to optimize the extract conditions of LVP based on single factor tests and O₂^-·, ·OH, DPPH, ABTS radicals scavenging activities were tested for antioxidant activity evaluation.The results showed that the optimal condition for extraction of LVP was as follows: temperature of 90 ℃, water bath time of 4 h, ratio of water to raw material of 33∶ 1 m L/g, corresponding yield of LVP was 2.18%, containing 55.40% LVP, 11.37% protein and no starch. The IC50 of O₂^-·, ·OH, ABTS and DPPH radicals scavenging activities of LVP were 868.16, 280.00, 342.06, 167.65 μg/m L respectively.All in all, response surface design is reliable for the study of relationship between yield of LVP and temperature, water bath time, ratio of water to raw material, furthermore, LVP shows a higher antioxidant activity in radical scavenging experiments.
- Lactarius Volemus /
- polysaccharides /
- extraction /
- response surface design /
- antioxidant activity

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参考文献(20)

[1]	王俊燕, 蒋世铮.阿尔泰山的优质食用菌-多汁乳菇[J].食用菌, 2002, 24 (5) :4.
[2]	计红芳, 杨谦, 宋瑞清.乳菇属真菌应用研究进展[J].林业科技, 2006, 31 (3) :28-30.
[3]	胡先运, 王传明, 江家志, 等.多汁乳菇的研究及应用[J].北方园艺, 2014, 18 (5) :157-160.
[4]	刘佳, 高敏, 殷忠.贵州野生多汁乳菇氨基酸成分及致小鼠突变性实验[J].预防医学情报杂志, 2007, 23 (2) :230-232.
[5]	柯丽霞.红汁乳菇和多汁乳菇的化学成分及其开发利用前景[J].安徽师范大学学报:自科版, 2000, 23 (4) :391-394.
[6]	胡先运, 王传明, 张根, 等.多汁乳菇人工繁育及抗肿瘤活性[J].中国实验方剂学杂志, 2014, 20 (22) :161-164.
[7]	Xie M H, Luo Z Z, Hu Y T, et al.Extraction and Antioxidant Activity Analysis of Crude Polysaccharides from Wild Lactarius volemus Fr.in Yunnan Province[J].Agricultural Biotechnology, 2015, 4 (6) :53-56.
[8]	张水花, 江永刚, 赵红艳.多汁乳菇多糖的微波辅助提取工艺及其清除DPPH自由基研究[J].北方园艺, 2016, 14:135-138.
[9]	董汝晶.多糖提取方法的研究进展[J].农产品加工 (学刊) , 2014, (8) :46-48, 51.
[10]	何雪梅, 孙健, 李丽, 等.响应面法优化蔗梢多糖超声波提取工艺[J].南方农业学报, 2014, (3) :458-462.
[11]	张志军, 刘建华, 李淑芳, 等.灵芝多糖纯度的苯酚硫酸法检测研究[J].食品工业科技, 2006, 34 (2) :193-195.
[12]	陈智慧, 史梅, 王秋香, 等.用凯氏定氮法测定食品中的蛋白质含量[J].新疆畜牧业, 2008, 12 (5) :22-24.
[13]	Tahmouzi S.Optimization of polysaccharides from Zagros oak leaf using RSM:antioxidant and antimicrobial activities[J].Carbohydrate Polymers, 2014, 106 (1) :238-246.
[14]	柳杨, 罗瑞明.长枣多糖水提工艺参数的响应面分析及优化[J].食品与机械, 2010 (5) :128-130.
[15]	Huang X Q, Tu Z C, Jiang Y, et al.Dynamic high pressure microfluidization-assisted extraction and antioxidant activities of lentinan[J].International Journal of Biological Macromolecules, 2012, 51 (5) :926-932.
[16]	张乃珣, 尹红力, 赵鑫, 等.红松多酚与真菌多糖联合清除ABTS自由基活性比较[J].北京林业大学学报, 2016, 35 (10) :104-111.
[17]	张颖, 潘俊羽.枇杷叶多糖水提醇沉法的提取条件优化[J].贵州农业科学, 2014 (6) :161-163, 167.
[18]	陈建斌, 李冲伟.响应面法优化紫苏多糖的水提工艺[J].食品研究与开发, 2014 (9) :27-31.
[19]	Du X J, Zhang Y, Mu H M, et al.Structural elucidation and antioxidant activity of a novel polysaccharide (TAPB1) from Tremella aurantialba[J].Food Hydrocolloids, 2015, 43 (4) :459-464.
[20]	Li Y T, Meng S L, Shi M, et al.Bioactivity Evaluation of Crude Polysaccharide from Rice Bran Fermented by Preussia Aemulans and the Changes in its Nutritional Contents[J].Journal of Food Biochemistry, 2016, 40 (5) :664-672.