基于纳米金标记-适配体识别对鱼中磺胺二甲氧嘧啶的定量检测

刘秀英; 李伊涵; 李巧素; 朱力杰; 高雪; 汤轶伟; 励建荣

doi:10.13386/j.issn1002-0306.2017.09.045

基于纳米金标记-适配体识别对鱼中磺胺二甲氧嘧啶的定量检测

渤海大学食品科学与工程学院辽宁省食品安全重点实验室生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心

基金项目:

国家自然科学基金项目(31601510)；辽宁省农业领域青年科技创新人才培养计划(2015001)；

详细信息

作者简介:
刘秀英 (1987-) , 女, 博士, 讲师, 研究方向:食品质量与安全, E-mail:xiuyingliu727@126.com。;

中图分类号: O657.3;TS254.7
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- 文章访问数: 156
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出版历程
- 收稿日期: 2016-10-27

Quantitative detection of sulfadimethoxine in fish based on the recognition of gold nanoparticles labeled aptamers

College of Food Science and Technology, Bohai University;Food Safety Key Lab of Liaoning Province;National & Local Joint Engineering Research Center of Storage, Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products

摘要

摘要: 纳米金因具有独特的光学效应,已成为极具应用前景的标记材料。适配体对目标分子具有较强特异识别能力,被广泛应用于分析检测领域。本文利用纳米金标记适配体,构建了一种磺胺二甲氧嘧啶的可视化检测新方法。利用该方法对磺胺二甲氧嘧啶进行定量分析,在添加水平分别为2、7 ng/m L下,测得回收率为89.86%和85.64%,日内日间相对标准偏差分别为4.00%、5.28%和5.88%、6.67%。方法的检测线性范围为1~10 ng/m L,检测限为1 ng/m L。以上结果表明该方法准确度高,精密度好,将其应用于鲈鱼、鲶鱼等实际水产样品中磺胺二甲氧嘧啶的定量分析具有可行性。
- 适配体 /
- 纳米金 /
- 磺胺二甲氧嘧啶 /
- 定量检测 /
- 水产品
Abstract: Gold nanoparticles ( Aunps) have been investigated as novel marking materials for their unique luminescent properties.Aptamer is a type of recognition materials with specific binding ability to capture target molecules and have been widely applied in the field of analysis and detection. In our study, a novel detection method for quantitative analysis of sulfadimethoxine was developed based on Au NPs and aptamer.The proposed method was applied to the analysis of real samples.The recoveries were 85.64% and 89.86%, and the relative standard deviation ( RSD) values were 4.00%, 5.28%, and 5.88%, 6.67%, for intra-and inter-day tests, respectively.The linear range of the method was 1¹0 ng/m L, and the detection limit was1 ng/m L.The above results suggested that the proposed method was highly accurate, and precise, and was feasible for the quantification of target sulfadimethoxine in aquatic product.
- aptamer /
- gold nanoparticles /
- sulfadimethoxine /
- quantitative detection /
- aquatic product

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参考文献(24)

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