Study on innovated continuous chromatographic process to separate L-tryptophan
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摘要: L-色氨酸发酵液的分离纯化是决定L-色氨酸生产成本和产品质量的重要因素,本研究旨在建立一种新型L-色氨酸连续色谱分离工艺,以降低L-色氨酸分离纯化成本,提高L-色氨酸的收率。通过单柱实验进行色谱填料筛选,并对L-色氨酸在不同树脂上吸附性能进行研究。结果表明,L-色氨酸在Applexion XA2014-22树脂上的动态载样量最高,达120 g/L树脂,确定以氨水为洗脱液,进行顺序式模拟移动床分离以后,L-色氨酸的纯度从30%提纯到80%左右,L-色氨酸收率达到99%,谷氨酸的去除率达到100%。本研究所得L-色氨酸发酵液分离提纯工艺和传统离子交换工艺相比,操作更为简单,产品纯度更高,能够有效降低工业化L-色氨酸生产成本,具有应用推广价值。Abstract: The separation and purification of L-tryptophan fermentation broth is an important factor forthe production cost of L-tryptophan industry.This study is aiming to develop an innovative chromatographic process to isolate L-tryptophan from fermentation broth in order to reduce the purification cost and increase the recovery of L-tryptophan. Continuous chromatographic process for L-tryptophan separation was set up by use of resin screening and sequential simulated moving bed ( SSMB) technology.Based on dynamic capacity of L-tryptophan on different resin, cation IEX resin of Applexion XA2014-22 was selected as the best one with dynamic capacity of 120 g L-Trp/L resin.After SSMB separation, 80% purity of L-tryptophan was obtained starting from 30% purity of raw material with selected resin and eluent by elution test. The final yield of L-tryptophan reached 99%, and all of the glutamic acid of raw material was removed.Compared with the traditional ion exchange process, this study provided an effective and economical technology for the separation and purification of L-tryptophan in industrial production.
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Keywords:
- L-tryptophan /
- sequential simulated moving bed /
- absorption /
- recovery
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