Optimization of the extraction of glycoprotein from bird's nest by response surface methodology
-
摘要: 通过水浸提法提取燕窝糖蛋白,以蛋白质提取率及多糖提取率为考察指标,研究浸提时间、料液比、浸提次数对燕窝糖蛋白提取效果的影响,并利用响应曲面法对燕窝糖蛋白提取工艺进行优化。结果表明:燕窝糖蛋白提取的最佳工艺条件为:浸提时间为2.6 h,料液比为1:47,浸提次数为2次,得到的蛋白质提取率为3.96%,多糖提取率为1.54%,提取液经浓缩沉淀,透析冻干后得到糖蛋白粗品,其得率为3.01%。另外,通过此法制备的糖蛋白粗品中蛋白质含量和多糖含量分别为59.06%和19.2%。Abstract: In this paper, glycoprotein from bird's nest was extracted by the hot water extraction.The effects of extraction time, material to liquid ratio and extraction times on the extraction rate of glycoprotein were studied, and the response surface analysis was used to optimize the extraction conditions of glycoprotein from bird's nest.The optimal condition of glycoprotein extraction was obtained with 2.6 hours of extraction time, 1 : 47 of material to liquid ratio and extracting for 2 times. The extracts were concentrated and precipitated. Finally, the crude glycoprotein was achieved by dialysis and lyophilization with the yield of 3.01%.In addition, the protein content and polysaccharide content in the crude glycoprotein prepared by this method were 59.06% and 19.2%, respectively.
-
Keywords:
- bird's nest /
- glycoprotein /
- extraction /
- response surface
-
[1] 任贻军, 王振军, 李万江.燕窝的鉴别研究概况[J].云南中医学院学报, 2009, 32 (4) :64-65. [2] Norhayati M K, Azman O, Nazaimoon W M.Preliminary Study of the Nutritional Content of Malaysian Edible Bird’s Nest[J].Malaysian Journal of Nutrition, 2010, 16 (3) .
[3] Wu Y, Chen Y, Wang B, et al.Application of SYBR green PCR and 2DGE methods to authenticate edible bird’s nest food[J].Food Research International, 2010, 43 (8) :2020-2026.
[4] 侯清娥, 董建伟, 王录军.糖蛋白的研究概述[J].农产品加工月刊, 2016 (1) :69-71. [5] 罗秋水, 上官新晨, 蒋艳, 等.超声波辅助提取紫红薯糖蛋白工艺的优化[J].中国食品学报, 2013, 13 (3) :110-114. [6] Ma F, Liu D.Sketch of the edible bird’s nest and its important bioactivities[J].Food Research International, 2012, 48 (2) :559-567.
[7] Tong T J, Liu D Y.Isolation and identification of glycopeptide in edible bird’s nest[J].Chin Sci Bull, 1985, 12:949-952.
[8] Nakagawa H, Hama Y, Sumi T, et al.Occurrence of a nonsulfated chondroitin proteoglycan in the dried saliva of Collocalia swiftlets (edible bird’s-nest) .[J].Glycobiology, 2007, 17 (2) :157-64.
[9] 由艳燕.燕窝唾液酸糖蛋白的纯化鉴定及体外消化吸收、抗炎活性研究[D].青岛:中国海洋大学, 2014. [10] 李晓龙.燕窝蛋白二维液相色谱分离方法的研究[D].天津:天津科技大学, 2010. [11] 张世伟, 赖心田, 陈血剑, 等.双抗夹心酶联免疫分析法检测燕窝中唾液酸糖蛋白[J].食品工业, 2013 (6) :195-198. [12] 崔慧娥, 李昌模, 张燕, 等.燕窝糖蛋白纯化及多克隆抗体的制备[J].食品工业科技, 2009 (7) :307-309. [13] You Y, Cao Y, Guo S, et al.Purification and identification ofα2-3 linked sialoglycoprotein andα2-6 linked sialoglycoprotein in edible bird’s nest[J].European Food Research and Technology, 2015, 240 (2) :389-397.
[14] 李婷婷, 张晖, 吴彩娥, 等.油茶籽糖蛋白提取工艺优化及抗氧化性[J].农业机械学报, 2012, 43 (4) :148-155. [15] 王倩, 刘淑集, 林彩平, 等.响应面法优化鱿鱼缠卵腺糖蛋白提取工艺研究[J].食品工业科技, 2014, 35 (3) :261-265.
计量
- 文章访问数: 141
- HTML全文浏览量: 16
- PDF下载量: 395
下载:
