响应曲面法优化燕窝糖蛋白提取工艺研究

Optimization of the extraction of glycoprotein from bird's nest by response surface methodology

摘要: 通过水浸提法提取燕窝糖蛋白,以蛋白质提取率及多糖提取率为考察指标,研究浸提时间、料液比、浸提次数对燕窝糖蛋白提取效果的影响,并利用响应曲面法对燕窝糖蛋白提取工艺进行优化。结果表明:燕窝糖蛋白提取的最佳工艺条件为:浸提时间为2.6 h,料液比为1:47,浸提次数为2次,得到的蛋白质提取率为3.96%,多糖提取率为1.54%,提取液经浓缩沉淀,透析冻干后得到糖蛋白粗品,其得率为3.01%。另外,通过此法制备的糖蛋白粗品中蛋白质含量和多糖含量分别为59.06%和19.2%。

Abstract: In this paper, glycoprotein from bird's nest was extracted by the hot water extraction.The effects of extraction time, material to liquid ratio and extraction times on the extraction rate of glycoprotein were studied, and the response surface analysis was used to optimize the extraction conditions of glycoprotein from bird's nest.The optimal condition of glycoprotein extraction was obtained with 2.6 hours of extraction time, 1 : 47 of material to liquid ratio and extracting for 2 times. The extracts were concentrated and precipitated. Finally, the crude glycoprotein was achieved by dialysis and lyophilization with the yield of 3.01%.In addition, the protein content and polysaccharide content in the crude glycoprotein prepared by this method were 59.06% and 19.2%, respectively.