Establishment of a rice protein sensitization animal model based on BN rat
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摘要: 以大米蛋白为过敏原,腹腔注射致敏雌性棕色挪威大鼠(BN大鼠),建立大米蛋白过敏BN大鼠动物模型。观察BN大鼠全身主动过敏反应及体重、体温、免疫脏器指数变化,检测其血清特异性Ig G、总Ig E和特异性Ig E水平,并测定血浆组胺释放水平及各组大鼠的血象变化。结果显示,实验第42 d大米蛋白激发实验后,实验组大鼠全部呈过敏反应阳性,且体温降低,脾脏指数显著增大(p<0.01),体内白细胞和嗜酸粒细胞数目升高(p<0.05);实验组血清特异性Ig G显著升高(p<0.01),总Ig E以及血浆组胺释放量均高于对照组(p<0.05),实验第14 d和42 d血清特异性Ig E高于对照组,第28 d无明显变化(p<0.05)。本实验结果证明BN大鼠是评价大米蛋白致敏性较为理想的动物模型。Abstract: In order to establish a sensitization animal model of rice protein, the sensitinogen was intraperitoneal injected to female Brown-Norway ( BN) rat.The general allergic reactions and the changes of weight, body temperature, immune organ index were observed, the level of antibody ( specific Ig G and Ig E, total Ig E) in sera, histamine and blood picture in plasma were measured to verify the animal model.Results showed that the general allergic reactions in each rat of experimental group were positive, the body temperature was decreased and the spleen index was increased significantly ( p < 0.01) , and white blood cells and acidophilic granulocyte number were increased ( p < 0.05) after stimulated by rice protein at the time of 42 nd day.Meanwhile, the level of specific Ig G was increased significantly ( p < 0.01) , total Ig E and plasma histamine was increased ( p < 0.05) compared to the control group, on the 14 th and 42 nd days, the level of specific Ig E was increased while it did not show obvious difference at the time of 28 th day ( p < 0.05) . This study indicates that the Brown Norway ( BN) rat is a suitable animal model for evaluating rice protein allergenicity.
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Keywords:
- rice protein /
- food allergen /
- animal model /
- antibody /
- histamine
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