石榴渣多酚提取及抗氧化活性研究

赵光远; 许艳华; 陈美丽; 纵伟

doi:10.13386/j.issn1002-0306.2017.05.035

石榴渣多酚提取及抗氧化活性研究

赵光远^1,2,
许艳华^1,2,
陈美丽^1,2,
纵伟^1,2

1. 郑州轻工业学院食品与生物工程学院
2. 食品生产与安全河南省协同创新中心

基金项目:

河南省食品加工过程安全控制技术创新团队(C20150024)；果蔬加工高新技术(16IRTSHN010)； 2015年徐州市高层次创新创业人才引进计划(2015)035号；

详细信息

作者简介:
赵光远 (1973-) , 男, 博士, 教授, 主要从事果蔬深加工方向, E-mail:guangyuan-zhao@163.com。;

纵伟 (1965-) , 男, 博士, 教授, 主要从事果蔬深加工方向, E-mail:zongwei1965@126.com。;

中图分类号: TS209
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出版历程
- 收稿日期: 2016-09-12

Extraction and antioxidant activity of polyphenols from pomegranate slag

College of Food and Bioengineering, Zhengzhou University of Light Industry
Collaborative Innovation Center of Food Production and Safety, Henan Province

摘要

摘要: 以石榴渣为实验原料,优化石榴渣多酚提取的工艺,并研究它的抗氧化性质。在单因素实验的基础上,利用响应面分析法(Response Surface Methodology,RSM)中的中心组合设计对石榴渣多酚的提取条件进行优化。用传统的溶剂提取法提取石榴渣中的多酚类物质,并利用福林酚法测定其含量。其抗氧化性用DPPH自由基清除能力和还原能力来评价。结果表明,最佳的石榴渣提取工艺条件是:41%的乙醇作溶剂,液料比20 m L/g,在62℃下提取3.5 h时多酚的得率最大为4.88 mg GAE/g,与预测值5.034 mg GAE/g接近。石榴渣多酚有较强的DPPH自由基清除率,且总抗氧化性与提取的石榴渣多酚浓度呈正相关,相关系数为0.9988。
- 石榴渣 /
- 响应面法 /
- 多酚 /
- 抗氧化
Abstract: To optimize the extraction of polyphenols from pomegranate seed and research its antioxidant activity.Methods: By traditional solvent extration method obtained the polyphenols in pomegranate seed, then determined by Folin-Ciocalteu ( FC) method.The levels of the four variables were optimized using response surface methodology ( RSM) with central composite design.The antioxidant activity was determined by 2, 2-Diphenyl-1-picrylhydrazyl ( DPPH) free radical scavenging ability and the assays of deoxidization capability.Results: The optimal conditions were determined as follows: extraction time of 3.5 h, extraction temperature of 62 ℃, ethanol concentration of 41%, and liquid-solid of 20 m L/g.Under these condition, the extraction value of polyphenols was 4.88 mg GAE/g material, which is accordance with the predicted value 5.034 mg GAE/g material.There is a positive correlation between polyphenols concentration from pomegranate seeds and DPPH free radical scavenging, and the correlation coefficient is 0.9988.
- pomegranate slag /
- response surface methodology /
- phenolics /
- antioxidant

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参考文献(19)

[1]	Geng S, Liu Y, Ma H J, Chen C G.Extraction and Antioxidant Activity of Phenolic Compounds from Okra Flowers[J].Tropical Journal of Pharmaceutical Research, 2015, 14 (5) :807-814.
[2]	Kaneria M J, Bapodara M B, Chanda S V.Effect of Extraction Techniques and Solvents on Antioxidant Activity of Pomegranate (Punica granatum L.) [J].Leaf and Stem, 2012, 5 (3) :396-404.
[3]	Singh R, Singh S, Kumar S, Arora S.Evaluation of antioxidant potential of ethyl acetate extract/fractions of Acacia auriculiformis A.Cunn[J].Food Chem Toxicol, 2007, 45:1216-1223.
[4]	Ardestani A, Yazdanparast R.Antioxidant and free radical scavenging potential of Achillea santolina extracts[J].Food Chem, 2007, 104 (1) :21-29.
[5]	Horuz E, Maskan M.Hot air and microwave drying of pomegranate (Punica granatum L.) arils[J].J Food Sci Technol, 2015, 52 (1) :285-293.
[6]	Tehranifar A, Zarei M, Nemati Z, Esfandiyari B, Vazifeshenas M R.Investigation of physico-chemical properties and antioxidant activity of twenty Iranian pomegranate (Punica granatum L.) cultivars[J].Sci Hortic-Amsterdam, 2010, 126 (2) :180-185.
[7]	Basiri S.Evaluation of antioxidant and antiradical properties of Pomegranate (Punica granatum L.) seed and defatted seed extracts[J].J Food Sci Technol, 2015, 52 (2) :1117-1123.
[8]	Fadavi A, Barzwgar M, Azizi M H.Determination of fatty acids and total lipid content in oil seed of 25 pomegranates varieties grown in Iran[J].J Food Compos Anal, 2006, 19 (6-7) :676-680.
[9]	Lansky E P, Newman R A.Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer[J].J Ethnopharmacol, 2007, 109 (2) :177-206.
[10]	朱彩平, 张艳霞, 张晓, 等.石榴皮多酚提取方法研究进展[J].食品与发酵工业, 2015, 41 (11) :243-248.
[11]	Fazaeli M, Yousefi S, Emam-Djomeh Z.Investigation on the effects of microwave and conventional heating methods on the phytochemicals of pomegranate (Punica granatum L.) and black mulberry juices[J].Food Research International, 2013, 50 (2) :568-573.
[12]	梁俊, 李建科, 刘永峰, 等.石榴皮多酚对脂变L-02肝细胞胆固醇合成的影响及机制探究[J].食品与生物技术学报, 2013, 32 (4) :487-493.
[13]	Chat O A, Najar M H, Mir M A, Rather G M, DAR AA.Effects of surfactant micelles on solubilization and DPPH radical scavenging activity of rutin[J].J Colloid Interface Sci, 2011, 355 (1) :140-149.
[14]	Strivastava A, Harish S R, Shivanandappa T.Antioxidant activity of the roots of Decalepis hamiltonii (Wight&Arn.) [J].LWT-Food Sci Technol, 2006, 39 (10) :1059-1065.
[15]	Liu B G, Ma Y X, Liu Y.Utrasonic-assisted extraction and antioxidant activity of flavonoids from Adinandra nitida leaves[J].Trop J Pherm Res, 2013, 12 (6) :1045-1051.
[15]	Lee W C, Yusof S, Hamid N S A, Baharin B S.Optimizing conditions for hot water extraction of banana juice using response surface methodology (RSM) [J].J Food Eng, 2006, 75 (4) :473-479.
[16]	Rafieian F, Keramat J, Kadivar M.Optimization of gelatin extraction from chicken deboner residue using RSM method[J].J Food Sci Technol, 2013, 50 (2) :374-380.
[17]	Guldiken B, Boyacioglu D, Capanoglu E.Optimization of extraction of bioactive compounds from black carrot using response surface methodology (RSM) [J].Food Anal Methods, 2016, 9 (7) :1876-1886.
[19]	Proestos C, Boziaris I S, Nychas C J E, Komaitis.Analysis of flavonoids and phnolic acids in Greek aromatic plants:Investigation of their antioxidant capacity and antimicrobial activity[J].Food Chem, 2006, 95 (4) :664-671.