响应面法优化红胞藻中藻红蛋白提取工艺及体外抗氧化性研究

孟晓; 张坤生; 张彦青; 温志友; 任云霞; 刘军军

doi:10.13386/j.issn1002-0306.2017.04.046

响应面法优化红胞藻中藻红蛋白提取工艺及体外抗氧化性研究

1. 天津商业大学生物技术与食品科学学院天津市食品生物技术重点实验室
2. 美国爱荷华州立大学食品科学与人类营养学院

基金项目:

天津特聘讲座教授科研项目资金；

详细信息

作者简介:
孟晓(1991-),男,硕士研究生,研究方向:食品加工与贮藏,E-mail:mengx9950@163.com。;

张坤生(1957-),男,博士,教授,研究方向:食品加工与贮藏,E-mail:zhksheng@tjcu.edu.cn。;

温志友(1971-),男,博士,教授,研究方向:生物能源、微藻研究,E-mail:wenz@iastate.edu。;

中图分类号: TS201.21
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出版历程
- 收稿日期: 2016-08-15

Optimization of ultrasonic wave extraction technique of phycoerythrin from Rhodophyta salina by response surface analysis and its antioxidant activity

School of Biotechnology and Food Science,Tianjin University of Commerce,Tianjin Key Laboratory of Food Biotechnology
Department of Food Science and Human Nutrition,Lowa State University

摘要

摘要: 利用响应面分析法优化藻红蛋白的提取工艺。在单因素实验基础上,使用硫酸铵分级沉淀提取纯化藻红蛋白,选超声时间、温度、功率为影响因素,以藻红蛋白得率为响应值,根据Box-Behnken实验设计原理对藻红蛋白提取工艺进行响应面分析,优化藻红蛋白提取工艺。结果表明:在超声时间为16 min、温度为33℃、功率为750 W时藻红蛋白得率取得最大值。在此优化条件下进行验证性实验,测得蛋白得率为15.93%,与预测值相对误差为2.50%,表明该优化工艺具有良好的可行性。藻红蛋白对O-2·和ABTS+·具有一定的清除作用,其IC₅₀分别为0.025 mg/m L和0.023 mg/m L。
- 响应面法 /
- 红胞藻 /
- 藻红蛋白 /
- 提取工艺 /
- 抗氧化性
Abstract: Response surface methodology was utilized to optimize ultrasonic wave extraction technique of phycoerythrin from Rhodophyta salina. Based on single factor test,ammonium sulfate fractionation precipitation was used to purify phycoerythrin with the tempreture,extraction power and extraction time as influential factors. A three- variable,Box- Behnken experiment design combined with response surface analysis was used to optimize the extraction technique. And the yield of phycoerythrin was selected as the response value.Results indicated that the optimal extraction process conditions were determined as 16 min,33 ℃ and 750 W for yield of phycoerythrin.Under this conditions,the yield of phycoerythrin was 15.93% with a 2.50% error compared with the actual value. Phycoerythrin had the ability to clear off superoxide free adicals( IC_(50)= 0.025 mg / m L) and ABTS free radical( IC50= 0.023 mg / m L)
- response surface methodology /
- Rhodophyta salina /
- phycoerythrin /
- extraction process /
- antioxidation activity

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