Inhibition and mechanism of Gnaphalium affine extract on the oxidation of edible oil during storage
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摘要: 为了研究鼠曲草提取物对油脂贮藏过程中氧化现象的抑制。通过测定油脂在贮藏过程中过氧化值的变化,观察鼠曲草提取物对油脂氧化酸败的抑制;采用分光光度法,进一步测定鼠曲草提取物对ABTS+和DPPH自由基的清除作用及其总酚和总黄酮含量,初步分析和探讨了其对油脂氧化的抑制机理。结果表明,鼠曲草提取物能显著地减弱食用油脂在贮藏过程中过氧化值的增加,对油脂的氧化酸败具有明显的抑制作用;初步分析和推测,鼠曲草提取物显著的自由基清除作用和较强的还原能力,及其较高的总酚（（169.9±7.7）mg没食子酸当量/g提取物）和总黄酮（（213.9±8.7）mg芦丁当量/g提取物）含量是其有效抑制油脂氧化酸败的重要原因。Abstract: The inhibition of Gnaphalium affine extract( GAE) against the oxidation of edible oil during storage was investigated.With the measurement of peroxidation value( POV),the inhibitory effect of GAE on the oxidation of edible oil during storage was determined. Then,the free radical( ABTS and DPPH) scavenging activities,reducing power and total phenolic and flavonoids content of GAE were also determined by using spectrophotometry,which were employed to explore the potential mechanism for its inhibition on oil oxidation. Present results showed that GAE exhibited the excellent capacity to weaken the POV increasing of edible oil during storage,which indicated that GAE possessed the remarkable inhibitory effect on the lipid oxidation.Furthermore,the significant free radical scavenging activity,strong reducing power and higher total phenolic( 169.9 ±7.7 mg gallic acid equivalent / g extract) and flavonoid( 213.9 ± 8.7 mg rutin equivalent / g extract) content of GAE were observed,which might be the important reasons responded for its effective inhibition on the oxidation of edible oil.
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Keywords:
- Gnaphalium affine /
- edible oil /
- storage process /
- oxidation /
- inhibitory mechanism
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