蝇蛆肽对衰老小鼠的作用及机制的初探

文雪; 魏洪; 赵春江; 朱丽华; 曹梅; 刘玉江

doi:10.13386/j.issn1002-0306.2017.03.062

蝇蛆肽对衰老小鼠的作用及机制的初探

贵州医科大学

基金项目:

贵州省2014年大学生创新创业训练计划项目(201410660019)；教育部2016年高校大学生创新创业训练计划项目(201610660310)；

详细信息

作者简介:
文雪(1995-),女,在读本科生,研究方向:功能食品开发,E-mail:1320123599@qq.com。;

魏洪(1971-),女,博士,副教授,研究方向:天然药物开发,E-mail:hongweitian-2001@163.com。;

中图分类号: R339.38
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出版历程
- 收稿日期: 2016-04-26

Study on fly maggot peptide on aging model mice and its mechanism

Guizhou Medical University

摘要

摘要: 目的:探讨蝇蛆肽对衰老小鼠的作用及其机制。方法:将60只小鼠随机分为6组,除空白组外,其余五组用D-半乳糖致衰老,分为衰老模型组、维生素C组以及蝇蛆肽低、中、高剂量组（2、4、8 g/kg）。测定小鼠的脾脏和胸腺指数。采用羟胺法测定小鼠肝脏和脑及血清中的超氧化物歧化酶（SOD）活力,可见光法测定过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）的活力,以硫代巴比妥酸（TBA）法测定丙二醛（MDA）的含量。结果:与空白组相比,模型组小鼠脾指数、胸腺指数及肝脏指数均有显著降低（p<0.05）,蝇蛆肽低、中、高剂量组脏器指数显著增高（p<0.05）。与空白组比较,模型组肝、脑中SOD、CAT、GSH-Px均降低,且MDA含量增高（p<0.05）,血清中SOD、CAT降低、MDA含量增高（p<0.05）;与模型组比较,蝇蛆肽低、中、高剂量组肝、脑组织中SOD、CAT、GSH-Px均显著增高、MDA含量显著降低（p<0.05）,血清中SOD、CAT显著增高、MDA含量显著降低（p<0.05）。结论:蝇蛆肽通过改变衰老小鼠的脏器指数而对其产生免疫调节作用,可显著提高小鼠肝脏和脑组织中的SOD、CAT、GSH-Px的活力,降低MDA的含量（p<0.05）,从而改变机体抗氧化能力。蝇蛆肽对衰老小鼠的影响机制可能与增强其体内抗氧化酶活力及改变脏器指数相关。
- 蝇蛆肽; /
- D-半乳糖; /
- 超氧化物歧化酶; /
- 过氧化氢酶; /
- 丙二醛;
Abstract: Objective: To study the effect of the fly maggot peptide on the of aging mice and the related mechanism.Methods: 60 mice were randomly divided into six groups.Except the blank group,the other five groups including aging model group,vitamin C group and fly maggot peptides of low,middle and high dose group( 2,4,8 g / kg / d) were induced aging with D- galactose.The spleen and thymus index of mice were measured. The hydroxylamine method was used to determine the superoxide dismutase( SOD) activity in mice's liver,brain and serum. The activity of catalase( CAT) and glutathione peroxidase( GSH- Px) was determined by the visible light method,the content of malondialdehyde( MDA) was determined using thiobarbituric acid( TBA)method.Results: Compared with the blank group,the spleen index,thymus index and liver index of model group were reduced significantly,and the fly maggot peptides of low,middle and high dose group organ index were significantly increased( p < 0.05).Compared with the blank group,the SOD,CAT,GSH- Px in liver,brain of model group were decreased,and MDA was increased( p < 0.05),CAT,SOD in serum were decreased,MDA content was increased( p < 0.05).Compared with the model group,SOD,CAT,GSH- Px in liver and brain tissue of low fly maggot peptides,and the high dose group were significantly increased,MDA content was decreased significantly( p < 0.05).SOD,CAT content in serum were significantly increased,MDA in serum was reduced( p < 0.05). Conclusions: Fly maggot peptides can affect immune function of aging mice by changing the organ index of aging mice,change the antioxidant capacity by increasing the activity of SOD,CAT,GSH- Px in liver and brain tissue and reducing the content of MDA.The influence mechanism of fly maggot peptides on aging mice may be associated with the increasing of the activity of antioxidative enzyme and organ index change.
- Fly maggot peptide; /
- D-galactose; /
- superoxide dismutase; /
- catalase malondialdehyde; /
- undefined

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参考文献(16)

[1]	Tang Y,Wang Y,Pei Z.A serine protease inhibitor from musca domestic larva exhibitsinhibitory activity against elastase and chymotrypsin[J].Biotechnology Letters,2016,38(7):1-7.
[2]	国果,吴建伟,付萍,等.家蝇幼虫分泌型抗菌肽对小鼠免疫功能的影响[J].中国公共卫生,2012,28(5):570-573.
[3]	焦丽萍.家蝇幼虫抗菌肽defensin基因的克隆、表达、纯化及抑菌、抑肿瘤活性研究[D].山西:山西医科大学,2013:61-74.
[4]	赵启龙,史彬林,张鹏飞,等.壳聚糖对鸡蛋品质及蛋黄抗氧化功能的影响[J].营养饲料,2015,51(11):60-63.
[5]	Juan Wang,Yansheng Wang,Xiangli Dang,et al.Housefly larvae hydrolysate:orthogonal optimization of hydrolysis,antioxidant activity,amino acid composition and functional properties[J].BMC Research Notes,2013,6(197):1-10.
[6]	徐淑云,陈修.药理实验方法学[M].第三版.北京:人民卫生出版社,2002:1464-1465.
[7]	王丽媛.家蝇幼虫酶解物的制备及其抗氧化活性的研究[D].大庆:黑龙江八一农垦大学,2010:33-46.
[8]	Fu P,Wu JW,Guo G,et al.Purification and molecularidentification of antifungal peptide from thehemolymph of Musca domestic(housefly)[J].Cellular and Molecular Immunology,2009,6(4):45-48.
[9]	宋晓骥,王立策.多肽固相合成[J].黑龙江科技信息,2011,(31):69-69.
[10]	黄双.D-半乳糖致小鼠衰老模型剂量和给药方式探讨[D].广西:广西医科大学,2014:17-49.
[11]	翟跃芬,吴海琴,吕多,等.EPO的抗衰老作用及其作用机制的初步探讨[J].四川大学学报(医学版),2012,43(5):679-682.
[12]	张钟,高红梅,叶林杰.玉米多糖的抗衰老作用[J].中国粮油学报,2014,29(7):18-21.
[13]	耿广琴,杨雅丽,王晶,等.党参水提取物对D-半乳糖致衰老模型小鼠肝肾SOD活性、MDA含量及超微结构的影响[J].中医研究,2014,27(7):70-74.
[14]	芦琳琳.红景天的抗衰老作用:口服红景天改善老龄大鼠免疫反应和增强谷氨酰半胱氨酸连接酶活性[D].山东:中国海洋大学,2013:24-44.
[15]	李玲玲.蝇蛆肽的酶法制备及其对大鼠营养作用、生物活性的研究[D].黑龙江:黑龙江八一农垦大学,2008:31-37.
[16]	杨春.外源性脂肪源干细胞移植改善D-半乳糖致衰老大鼠性能力的研究[D].广东:南方医科大学,2013:20-24.

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