酸水解-全自动氨基酸分析仪测定方格星虫中氨基酸
Determination of amino acids from Sipunculs nudus by acid hydrolysis-automatic amino acid analyzer
-
摘要: 目的:优化测定方格星虫氨基酸的实验条件。方法:研究以酸水解-全自动氨基酸分析仪分析法对海南东方方格星虫中18种氨基酸含量进行分析测定,为确保该实验结果的有效性与可行性,辅以线性关系,加标回收率实验,精密度实验,方法检出限的计算分析。结果:实验发现方格星虫样品中含有18种氨基酸,其中,鲜方格星虫中天门冬氨酸、精氨酸含量较高,分别为2.02%、1.26%,胱氨酸、色氨酸含量最低,分别为0.21%。方格星虫干中谷氨酸、精氨酸含量最高,分别为11.89%、7.94%,而胱氨酸、色氨酸含量最低,分别为0.45%、1.07%;结论:实验方案具备可操作性及数据有效性,仪器的精密度高,相对标准偏差在0.21%以下,检出限低,最低达到0.00000001 ng/kg,线性范围宽,且相关系数在0.9999以上,操作简便,结果令人满意。Abstract: Objective: To optimize determination experimental conditions of amino acids from Sipunculs nudus. Method: The author determined the contents of 18 kinds of amino acid by acid hydrolysis- automatic amino acid analyzer with calculation of regression equations and detection limit,experiment of precision and standard recovery,in order to ensure the effectiveness and feasibility of the experimental results.Results: It contains 18 amino acids. In fresh worm the contents of aspartic acid( 1.26%)and arginine( 2.02%) are the higher,and the contents of cystine( 0.21%) and tryptophan( 0.21%) are the lowest. In dry worm the contents of glutamic acid( 11.89%) and arginine( 7.94%) are the highest,and the contents of cystine( 0.45%) and tryptophan( 1.07%) are the lowest.Conclusion: The experiment scheme has good effect,simple operation,strong operability and valid data,owing to its instrument of high precision and relative standard deviation below 0.21%,low detection limit and the minimum is 0.00000001 mg / kg,wide linear range and the correlation coefficient over 0.9999,so the result is satisfactory.
下载: