Effect of whey protein isolate on clarification of fresh Pyrus bretschneideri ‘Huangguan'pear juice
-
摘要: 为了探究乳清分离蛋白(whey protein isolate,WPI)对鲜榨黄冠梨汁的澄清效果,在单因素实验的基础上应用响应面法对鲜榨梨汁的澄清条件进行优化,并对比研究WPI、果胶酶、WPI+果胶酶3种处理方法对梨汁澄清度和褐变度的影响。优化的澄清条件为WPI添加量0.69%,处理时间90 min,处理温度60℃,该条件下梨汁透光率为87.9%。3种方法处理后梨汁澄清度的大小顺序为WPI+果胶酶>WPI>果胶酶,褐变度的大小顺序为果胶酶>WPI>WPI+果胶酶。这表明WPI对鲜榨黄冠梨汁具有澄清和抑制褐变的双重作用,效果优于果胶酶,与果胶酶联合处理,效果更佳。Abstract: The effect of whey protein isolate( WPI) on clarification of fresh‘Huangguan'pear juice was evaluated.Based on the single factor experiments,the response surface methodology was employed to optimize the clarification conditions of pear juice and the effects of WPI and pectinase treatments alone and combined on clarity and browning degree of pear juice were also studied.The optimum conditions for clarification of pear juice were as follows: the concentration of WPI 0.69%,treatment time 90 min and treatment temperature 60 ℃.Under the conditions,the transmittance of pear juice was 87.9%.The order of clarity of pear juice treated by the three methods was WPI- pectinase > WPI > pectinase. The order of browning degree of pear juice treated by the three methods was pectinase > WPI > WPI- pectinase. The results suggested that WPI had dual effects of clarification and inhibition of browning on fresh‘Huangguan'pear juice.Moreover,WPI acted more effectively than pectinase.In addition,the effect of WPI combined with pectinase treatment was better than the effect of WPI treatment alone.
-
Keywords:
- pear juice /
- whey protein isolate /
- pectinase /
- response surface /
- clarification
-
[1] Xie D,Zhong HY,Mo J,et al.Nutritional and medicinal quality of pear juice:next hotspot?[J].Food,2007,1(1):41-48.
[2] Markowski J,Zbrze z'niak M,Mieszczakowska-Frc M,et al.Effect of cultivar and fruit storage on basic composition of clear and cloudy pear Juices[J].LWT-Food Science and Technology,2012,49(2):263-266.
[3] 邱静,邹礼根,赵芸,等.梨汁加工工艺关键技术[J].农产品加工·学刊,2012,(3):80-83. [4] 夏玉静,王文辉,贾晓辉,等.梨果加工制品市场调查情况分析[J].中国农学通报,2009,25(22):340-343. [5] 赵会英.黄冠梨优质丰产栽培技术[J].河北林业科技,2008,(2):68. [6] 李丽梅,赵哲,何近刚,等.不同品种梨果实酚类物质和抗氧化性能分析[J].食品科学,2014,35(17):83-88. [7] 马文会,樊庆耀,黄兰计,等.黄冠梨鸡爪病发病特点研究[J].河北农业科学,2008,11(1):29-31. [8] 曹智.黄冠梨:为何失去“桂冠”[N].河北日报,2015-9-24(6). [9] 张亚伟,陈义伦.不同品种梨汁酶促褐变因子及相关性[J].中国农业科学,2011,44(9):1880-1887. [10] 孙珊珊,陈义伦,高二东.雪梨汁护色、澄清技术研究[J].食品工业科技,2011,32(1):204-206. [11] Hsu J C,Heatherbell D A,Yorgeybrian M.Effects of variety,maturity and processing on pear juice quality and protein stability[J].Journal of Food Science,1990,55(6):1610-1613.
[12] 卢晓明,王静波,任发政,等.乳清蛋白在食品工业中的应用[J].食品科学,2010,31(1):262-267. [13] Yi J H,Ding Y.Dual effects of whey protein isolates on the inhibition of enzymatic browning and clarification of apple juice[J].Czech Journal of Food Sciences,2014,32(6):601-609.
[14] Veleirinho B,Lopes-da-Silva JA.Application of electrospun poly(ethylene terephthalate)nanofiber mat to apple juice clarification[J].Process Biochemistry,2009,44(3):353-356.
[15] Elizabeth F.Whey protein builds on success[J].Beverage Industry,2011,102(4):62-64.
[16] 廖红梅,丁占生,钟葵,等.高压二氧化碳对鲜榨梨汁贮藏稳定性的影响研究[J].食品工业科技,2013,34(23):131-142. [17] 黄文书,曹虹.库尔勒香梨汁护色与澄清技术的研究[J].保鲜与加工,2014,14(5):45-48. [18] Livney YD.Milk proteins as vehicles for bioactives[J].Current Opinion in Colloid and Interface Science,2010,15(1/2):73-83.
[19] Haslam E,Lilley T H,Cai Y,et al.Traditional herbal medicines-The role of polyphenols[J].Planta Medica,1989,55(1):1-8.
[20] 赵新淮,于国萍,张永忠,等.乳品化学[M].北京:科学出版社,2007:293-295. [21] 白雨鑫,郭斌,韩冠英,等.萝藦果壳多糖提取工艺优化及其抗氧化活性研究[J].食品工业科技,2015,36(20):278-283. [22] 蒲彪,胡小松.饮料工艺学[M].2版.北京:中国农业大学出版社,2009:121. [23] 易建华,朱丹,朱振宝.乳清分离蛋白对苹果汁澄清效果的研究[J].食品与机械,2013,29(5):41-50. -
期刊类型引用(1)
1. 韦艳萍,谭栀恩,陀海燕,曾佩琴,刘雅婷,覃柳馨,易蔚,陈卫卫. 星点设计-效应面法优选小儿益肺健脾煎膏剂提取工艺研究. 中国医药导报. 2021(01): 13-17+35+197 . 百度学术
其他类型引用(1)
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
- 被引次数: 2