Purification of flavonoids from wheat germ by macroporous resin
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摘要: 在前期研究麦胚黄酮最佳浸提工艺基础上,为探讨麦胚黄酮纯化工艺,本实验选择大孔树脂对其进行分离纯化。以吸附能力、吸附率及解吸率为考察指标,从7种型号大孔树脂中筛选出分离纯化麦胚黄酮效果优的树脂,并确定该树脂的最佳工艺条件。结果表明,H103大孔树脂的吸附率、吸附能力都较高,为麦胚黄酮最佳分离树脂,其最佳工艺条件为上样浓度约0.65 mg/m L、上样速度2.0 BV/h、解吸乙醇浓度70%、解吸速度2.0 BV/h。经H103树脂分离后的麦胚黄酮纯度大大提高,为11.77%,比浸提液中麦胚黄酮纯度0.96%提高了12.26倍。Abstract: Based on the previous study of the extraction of flavonoids from wheat germ( FWG),the purification of FWG was performed by macroporous resin.Seven kinds of macroporous resin were selected to compare their purification ability by using adsorption and desorption rate as the indicators.Furthermore,the best one was used to optimize the separation process.The data indicated that H103 had better adsorption and desorption property for purification. The optimal adsorption parameters were as follows: the sample concentrations was about 0.65 mg / m L,the flow rate was 2.0 BV / h,the desorption solution concentration was70% ethanol,the desorption rate was 2.0 BV / h.After one run treatment with H103 resin,the extraction of FWG was increased12.26 times,and the content of flavonoids was up to 11.77% from 0.96%.
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Keywords:
- wheat germ flavonoids /
- macroporous resin /
- purification
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