Extraction of total phenolics and flavonoids from the leaf of Ribes odoratum Wendl.and its antioxidant activity
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摘要: 目的:获得微波辅助提取香茶藨子叶片中总酚及总黄酮的最佳工艺,以及香茶藨子叶片中总酚及总黄酮的体外抗氧化活性。方法:在单因素的基础上,采用响应面法对影响总酚和总黄酮提取量的主要因素进行分析,探讨微波辅助提取香茶藨子叶片中总酚及总黄酮的最优工艺,并与超声提取、索氏提取进行比较。采用DPPH和FRAP法对香茶藨子叶片中总酚及总黄酮的自由基清除能力和总抗氧化活性进行评价。结果:最佳的提取工艺条件为:提取温度58℃,液固比39∶1 m L/g,乙醇浓度57%,提取时间15 min和微波功率600 W,此条件下总酚及总黄酮的提取量分别为99.89、38.16 mg/g。微波辅助提取得到的香茶藨子叶片提取物对DPPH自由基有很好的清除作用,IC50值为0.0445 mg/m L,此外其还有较高的总抗氧化能力,FRAP值为4.832 mmol/g。结论:优选的总酚、总黄酮提取工艺稳定可行,香茶藨子叶片中总酚及总黄酮有较好的抗氧化活性,为其研究开发提供一定的科学依据。Abstract: Objective: To obtain the optimum microwave- assisted extraction process for total phenolics and flavonoids from the leaf of Ribes odoratum Wendl. and to examine the antioxidant activity. Method: Based on single factor tests,effects of various factors on total phenolics and flavonoids contents were analyzed through response surface methodology( RSM).The optimal conditions for microwave extraction of total phenolics and flavonoids from the leaf of Ribes odoratum Wendl.were discussed.And the comparison between different extraction methods,such as ultrasonic extraction and soxhlet extraction were also investigated.Moreover,the DPPH radical scavenging capacity and FRAP total antioxidant activity tests were carried out to determine its potential antioxidant activity. Result: The optimum conditions for microwave- assisted extraction were determined as follows:extraction temperature 58 ℃,liquid- to- solid ratio 39 ∶ 1 m L / g,ethanol concentration 57%,extraction time 15 min and microwave power 600 W.The extraction yields of total phenolics and flavonoids were up to 99.89 mg / g and 38.16 mg / g under the optimal conditions. The antioxidant tests showed that the total phenolics and flavonoids extracted from the leaf of Ribes odoratum Wendl.had a potent scavenging effect on DPPH free radicals with IC50 value of 0.0445 mg / m L and FRAP ferric reducing antioxidant power with value of 4.832 mmo L / g.Conclusion: The microwave- assisted extraction of total phenolics and flavonoids from the leaf of Ribes odoratum Wendl.was stable and feasible,and the extracts by this method also exhibited a betterantioxidant activity.The study could provide a certain scientific basis for research and development of Ribes odoratum Wendl..
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