Ultrasound-assisted extraction and antioxidant activities in vitro of total flavonoids from Caulis Lonicerae
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摘要: 本研究采用中心复合实验优化了超声辅助提取忍冬藤总黄酮的工艺;以1,1-二苯基-二苦基肼（1,1-diphenyl-2-picrylhydrazyl,DPPH）自由基和2,2’-连氮基-双-（3-乙基苯并噻唑-6-磺酸）（ABTS+·）清除能力,Fe2+螯合力和铁离子还原抗氧化力（ferric reducing antioxidant power,FRAP）为指标,研究了忍冬藤总黄酮的体外抗氧化活性。结果表明:超声辅助提取忍冬藤总黄酮的最佳提取工艺为:乙醇浓度70%、液固比31∶1（m L/g）、超声功率210 W,超声时间8 min,按此工艺提取忍冬藤总黄酮,得率达7.74%±0.10%。体外抗氧化活性的研究显示忍冬藤总黄酮清除DPPH自由基的IC50为48.1μg/m L、清除ABTS+自由基的IC50为66.1μg/m L、Fe2+螯合力的EC50为83.6μg/m L,在6.25200μg/m L的浓度范围内,FRAP值与总黄酮的浓度呈线性关系:Y=0.0019x+0.1527（R2=0.9964）。此研究为忍冬藤总黄酮的进一步开发和利用提供了理论依据。Abstract: The optimization of ultrasound- assisted extraction process of total flavonoids from Caulis Lonicerae was investigated by response surface methodology( RSM) based on central composite design and the antioxidant activities in vitro of the extracted flavonoids was measured using 1,1- diphenyl-2- picrylhydrazyl( DPPH·) scavenging,2,2'- azino- bis( 3- ethylbenzthiazoline-6- sulfonic acid( ABTS+·) radical scavenging,Fe2 +chelating activity and ferric reducing antioxidant power( FRAP) methods.The results showed that the optimal ultrasound- assisted extraction conditions for total flavonoids from Caulis Lonicerae were obtained as follows: ethanol concentration was 70%,liquid- solid ratio 31 ∶ 1( m L / g),ultrasonic power 210 W,ultrasonic time8 min. Under these conditions,the yield of total flavonoids was 7.74% ± 0.10%. The extracted flavonoids exhibited powerful antioxidant activities in vitro. The IC50 of 1,1- diphenyl- 2- picrylhydrazyl( DPPH radical) scanvenging and ABTS+radical scanvenging capcities were 48.1,66.1 μg / m L,respectively. The EC50 of Fe2 +chelating ability was 83.6 μg / m L,and in the concentration range of 6.25~200 μg / m L,the linear regression equation of relationship between TRAP value and total flavonoids concentration from was as follow: Y = 0.0019 x + 0.1527( R2= 0.9964). This research might provide a theoretical basis for the further development and utilization of total flavonoids from Caulis Lonicerae.
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Keywords:
- Caulis Lonicerae /
- total flavonoids /
- ultrasound-assisted /
- antioxidant
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