植物乳杆菌耐受口腔不良环境及其抑菌特性研究

郭夏蕾; 张健; 杨贞耐

doi:10.13386/j.issn1002-0306.2017.02.033

植物乳杆菌耐受口腔不良环境及其抑菌特性研究

北京食品营养与人类健康高精尖创新中心,食品质量与安全北京实验室北京工商大学

基金项目:

国家自然科学基金面上项目(31571857)；

详细信息

作者简介:
郭夏蕾(1991-),女,硕士研究生,研究方向:乳品生物技术,E-mail:610850207@qq.com。;

杨贞耐(1965-),男,博士,教授,研究方向:乳品科学及加工技术,E-mail:yangzhennai@th.btbu.edu.cn。;

中图分类号: TS201.3
计量
- 文章访问数: 198
- HTML全文浏览量: 30
- PDF下载量: 209
出版历程
- 收稿日期: 2016-09-18

Study on oral application characteristics of lactic acid bacteria that inhibiting Streptococcus mutans

Beijing Advanced Innovation Center for Food Nutrition and Human Health,Beijing Laboratory of Food Quality and Safety,Beijing Technology and Business University

摘要

摘要: 拟利用前期已经筛选出的可以抑制变异链球菌的植物乳杆菌,研究了能抑制变异链球菌的植物乳杆菌在口腔应用方面的特性的研究。本研究测定了植物乳杆菌对溶菌酶、抗生素、盐和酸的耐受性,测定了五株植物乳杆菌（Lactobacillus plantarum,L.plantarum）K25、SKT109、YW3-2、YW5-1和YW1-1的自聚集能力以及分别与变异链球菌的共聚集能力,以及研究了添加植物乳杆菌对变异链球菌自聚集能力和产胞外多糖的影响。结果表明,五株植物乳杆菌对溶菌酶都有一定耐受性,远高于人体口腔唾液的溶菌酶浓度（157μg/m L）。五株植物乳杆菌对酸和盐也有一定的耐受性。L.plantarum YW5-1对抗生素的耐受性较强。添加L.plantarum K25、YW5-1和YW1-1上清液后可以降低变异链球菌合成胞外多糖的能力。5株植物乳杆菌中K25和YW5-1的自聚和共聚能力较强,并且添加了植物乳杆菌后,可以降低变异链球菌的自聚集能力。
- 植物乳杆菌 /
- 变异链球菌 /
- 口腔应用 /
- 抑菌活性
Abstract: Intends to use the pre- screened lactic acid bacterias that can inhibit Streptococcus mutans,oral application characteristics of lactic acid bacteria that inhibiting Streptococcus mutans were studied.The study measured lysozyme,antibiotic,salts and acid tolerance of L. plantarums,measured the auto- aggregation ability and co- aggregation ability of five lactic acid bacteria( Lactobacillus plantarum,L.plantarum) K25,SKT109,YW3-2,YW5-1 and YW1-1 with S.mutans.And the infuence of L.plantarum on auto- aggregation and exopolysaccharide synthesis of Streptococcus mutans was determined. The results showed that five lactic acid bacteria have a certain tolerance for lysozyme,much higher than the concentration of lysozyme in human saliva( 1~57 μg / m L).Five lactic acid bacteria also had a certain tolerance for salts and acid.L.plantarum YW5-1 showed the best tolerance to antibiotics.Adding supernatant of L.plantarum K25,YW5-1 and YW1-1 could reduce the ability of S.mutans to synthesizing exopolysaccharide.The auto- aggregation ability and co- aggregation of YW5- 1 and K25 were best of the five Lactobacillus plantarum( L.plantarum),and after adding lactic acid bacteria,auto- aggregation ability of S. mutans could be reduced.
- Streptococcus mutans /
- Lactobacillus plantarum /
- oral application /
- inhibitory activity

HTML全文

参考文献(24)

[1]	P E Kolenbrander,NS Jakubovics,G Bachrach.Oral Microbiology[J].Encyclopedia of Microbiology,2009:566-588.
[2]	马旭东,杨景云.口腔微生态失衡导致口腔异味的研究[J].中国微生态学杂志,2008,20(5):480-482.
[3]	Rugg A.Dental caries:strategies to control this preventable disease[J].Acta Medica Academica,2013,42(2):117-130.
[4]	周学东.龋病学[M].北京:人民卫生出版社,2010:5-8.
[5]	Dewitte S N,Bekvalac J.Oral health and frailty in the medieval English cemetery of St Mary Graces[J].American Journal of Physical Anthropology,2010,142(3):341-354.
[6]	Banas JA.Virulence properties of Streptococcus mutans[J].Frontiers in Bioscience,2004(9):1267-1277.
[7]	Sela MN,Steinberg D,Segal R.Inhibition of the activity of glucosyltransferase from Streptococcus mutans by gly-cyrrhizin[J].Oral Microbiol Immunol,1987,2(3):125-128.
[8]	杨隆强,周乔,刘敏川.变异链球菌黏附机制的研究进展[J].国际口腔医学杂志,2011,38(2):229-233.
[9]	Vrese M D,Schrezenmeir J.Probiotics,Prebiotics and Synbiotics[J].Advances in Biochemical Engineering Biotechnology,2008,111:1-66.
[10]	Meurman JH.Probiotics:do they have a role in oral medicine and dentistry?[J].European Journal of Oral Sciences,2005,113(3):188-196.
[11]	王胜朝,许浩坤.口腔益生菌防龋作用与应用[J].中国实用口腔科杂志,2012,10(5):587-590.
[12]	Ishikawa H,Aiba Y,Nakanishi M,et al.Suppression of periodontal pathogenic bacteria in the saliva of human by the administration of Lactobacillus salivarius TI2711[J].J Jap Soc Periodontol,2003,45(1):105-112.
[13]	Caglar E,Sandalli N,Twetman S,et al.Effect of yogurt with Bififi-dobacterium DN-173 010 on salivary mutans streptococci and lactobacilli in young adults[J].Acta Odontol Scand,2005,63(6):317-320.
[14]	Caglar E,Cildir S K,Ergeneli S,et al.Salivary mutans streptococci and lactobacilli levels after ingestion of the probiotic bacterium Lactobacillus reuteri ATCC 55730 by straws or tablets[J].Acta Odontologica Scandinavica,2006,64(5):314-318.
[15]	郭夏蕾,张健,杨贞耐.抑制口腔变异链球菌的乳酸菌的筛选及其抑菌机理初探[J].食品科学,2016,37(19):118-123.
[16]	Kll P,Mndar R,Marcotte H,et al.Characterization of oral lactobacilli as potential probiotics for oral health[J].Oral Microbiol Immun,2008,23(2):139-147.
[17]	Guglielmetti S,Taverniti V,Minuzzo M,et al.Oral bacteria as potential probiotics for the pharyngeal mucosa[J].Appl Environ Microbiol,2010,76(12):3948-3958.
[18]	周学东.口腔生物化学[M].成都:四川大学出版社,2002:253.
[19]	Reid G,Mc Groarty J A,Angotti R,et al.Lactobacillus inhibitor production against Escherichia coli and coaggregation ability with uropathogens[J].Can J Microbiol,1988,34(3):344-351.
[20]	Collado M,Meriluoto J,Salminen S.Adhesion and aggregation properties of probiotic and pathogen strains[J].Eur Food Res Technol,2008,226(5):1065-1073.
[21]	Bosch M,Nart J,Audivert S,et al.Isolation and characterization of probiotic strains for improving oral health[J].Archives of Oral Biology,2012,57(5):539-549.
[22]	周菊芬,徐岩英,李晓新.口腔念珠菌病患者唾液溶菌酶水平的研究[J].北京口腔医学,2003,11(2):97-103.
[23]	常广双,李广鹏,刘政.龋齿预防的益生菌方法[J].乳品科学与技术,2011,34(6):288-292.
[24]	刘虹,翦祎,张化朋,等.口腔益生菌在口腔疾病防治上的研究及应用[J].药学研究,2015,34(6):357-360.