荧光光谱法研究咖啡酸与胰脂肪酶相互作用

范金波; 王晓露; 姜海静; 周素珍

doi:10.13386/j.issn1002-0306.2017.02.020

荧光光谱法研究咖啡酸与胰脂肪酶相互作用

1. 渤海大学食品科学与工程学院,辽宁省食品安全重点实验室,生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心
2. 唐山市丰润区综合职业技术教育中心

详细信息

作者简介:
范金波(1977-),男,博士,副教授,主要从事食品生物化学方面的研究,E-mail:jinbo_fan@hotmail.com。;

中图分类号: R96;O657.31
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出版历程
- 收稿日期: 2016-06-29

Study on the interaction between caffeic acid and pancreatic lipase by fluorescence spectroscopy

College of Food Science and Technology,Bohai University,Food Safety Key Lab of Liaoning Province,National & Local Joint Engineering Research Center of Storage,Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products
The Center of Comprehensive Occupation Technology Education in Fengrun District of Tangshan City

摘要

摘要: 采用荧光光谱法研究了咖啡酸与胰脂肪酶的相互作用,主要包括荧光猝灭特性及结合常数、结合位点数的计算,抑制酶类型及抑制活性,金属离子对相互作用的影响。结果表明:咖啡酸加入后胰脂肪酶产生了规律性的荧光猝灭,最大吸收波长出现红移现象,主要属于静态猝灭。25℃和37℃下结合常数分别为6.48×104L/mol和1.38×105L/mol,热力学参数ΔH>0且ΔS>0,表明结合反应是自发进行的吸热反应,相互作用主要表现为疏水作用力。咖啡酸对胰脂肪酶的抑制类型为非竞争性抑制,抑制活性IC50为（0.88±0.07）mg/m L。四种金属离子（Cu2+、Ca2+、Fe3+、Fe2+）一定程度上增加结合常数和结合位点数,说明四种金属离子促进了咖啡酸与胰脂肪酶的结合。
- 胰脂肪酶 /
- 咖啡酸 /
- 荧光猝灭 /
- 金属离子 /
- 抑制活性
Abstract: The interaction between chlorgenic acid and porcine pancreas lipase( PPL) was researched by fluorescence spectrophotometer,including the fluorescence quenching properties,calculating the binding constants and binding sites,inhibitory activity and inhibitory type of caffeic acid on porcine pancreatic lipase,effect of metal ions on the interaction.Experimental results showed that caffeic acid of had strong fluorescence quenching effect on porcine pancreas lipase,maximum absorption wavelength redshift,mainly belongs to static quenching. Binding constant under 25 ℃ and 37 ℃ 6.48 × 104 L / mol and 1.38 × 105 L / mol respectively,and the thermodynamic parameters Δ H > 0 and Δ S > 0,showed that combination was a spontaneous endothermic reaction,the main force of hydrophobic forces.Inhibitory type of caffeic acid on pancreatic pancreatic lipase was noncompetitive inhibition,inhibitory activity was IC50( 0.88 ± 0.07) mg/m L.When there were four kinds of metal ion( Cu2 +,Ca2 +,Fe3 +,Fe2 +) binding constant and binding points were increased,that metal ions,to a certain extent,promoted the combination of caffeic acid and pancreatic lipase.
- pancreas lipase /
- caffeic acid /
- fluorescence quenching /
- metal ion /
- inhibitory activity

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