Study on antioxidant peptide activity and functional properties of Rumex leaf protein
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摘要: 本文旨在探究鲁梅克斯叶蛋白酶解物的抗氧化性能和叶蛋白的功能性质,为鲁梅克斯叶蛋白的应用和推广辅以理论依据。研究选用碱热法获取的叶蛋白粉,分别采用胰蛋白酶和胃蛋白酶水解,对其酶解物进行自由基的清除能力和对金属离子的螯合能力评价;另外,本研究中还探讨了不同的温度、p H、Na Cl浓度对鲁梅克斯叶蛋白溶解性、持水性、持油性、发泡性及乳化性的影响。结果表明,在肽浓度分别为0.5、1、8、0.2 mg/m L时,胰蛋白酶酶解肽的还原力、对DPPH·的抑制率、对·OH的清除率和对Fe2+的螯合能力显著高于胃蛋白酶酶解肽（p<0.05）;而当肽浓度为80 mg/m L时,胃蛋白酶酶解肽对O-2·的抑制率则显著高于胰蛋白酶酶解肽（p<0.05）。二者在对自由基种类和清除效果上出现的差异,可能与酶解所得肽段中疏水性氨基酸的种类和数量有关。叶蛋白的功能性质研究表明,鲁梅克斯叶蛋白具有较高的持水性（均大于4 g/g）和发泡性（最高可达45%）,二者受温度、p H和Na Cl浓度的影响较小;而溶解性、持油性和乳化性受温度、p H和Na Cl浓度影响,表现出规律性差异,存在一定幅度上的波动。Abstract: This article aimed to explore the antioxidant properties of Rumex leaf protein hydrolyzate and Rumex leaf protein functional properties,which could provide a theoretical basis for Rumex leaf protein application and promotion.In this study,the leaf proteins obtained by alkaline heat treatment were hydrolyzed by trypsin and pepsin separately,and the hydrolysates ability of free radical scavenging and chelated metal ions were evaluated. In addition,the study also discussed the effect of different temperature,p H,Na Cl concentration on Rumex leaf protein solubility,water binding capacity,oil binding capacity,foaming and emulsifying properties.The results showed that,when the peptide concentrations were 0.5,1,8,and 0.2 mg / m L separately,the reducing power,the scavenging ability of DPPH,·OH and Fe2 +chelating ability of tryptic peptides were significantly higher than pepsin peptides( p < 0.05). When the peptide concentration was 80 mg / m L,pepsin peptides on the scavenging O-2·significantly was higher than the tryptic peptides. The differences in the types and effects of free radical scavenging of the two peptides might be related to the type and quantity of hydrophobic amino acids in the peptides derived from the enzymatic hydrolysis.Functional properties of leaf protein showed that Rumex leaf protein was of high water binding capacity( > 4 g / g) and foaminess( up to a maximum of 45%),which were less affected by temperature,p H and Na Cl concentration. However,the solubility,oil bingding capacity and emulsifying affected by temperature,p H and Na Cl concentration showed the regular difference and fluctuation a certain range.
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Keywords:
- Rumex /
- leaf protein /
- antioxidant peptides /
- functional properties
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[1] 张艺,李忠.植物叶蛋白的开发应用[J].食品研究与开发,2004(5):103. [2] 谢正军.苜蓿叶蛋白和酶法制备抗氧化肽的研究[D].无锡:江南大学,2009:68. [3] 刘洪对.五种花生抗氧化肽体外抗氧化活性比较[J].核农学报,2013,27(8):1162-1167. [4] 陈日春.鲢鱼鱼麟胶原蛋白肽的制备及其抗氧化活性的研究[D].福州:福建农林大学,2013(41):52-64. [5] 陈英,朱科学,彭伟,等.小麦胚活性肽体外抗氧化活性研究[J].中国油脂,2010,35(12):17-19. [6] 刘建超,孙荣欣,刘飏.花生肽体外抗氧化活性的研究[J].三门峡职业技术学院学报,2010(4):112-114. [7] 刘文颖,谷瑞增,鲁军,等.大豆低聚肽的成分分析及体外抗氧化作用[J].食品工业,2015(4):200-203. [8] 綦蕾,王振宇.红松松仁抗氧化肽的制备及体外抗氧化活性评价[J].食品与发酵工业,2010,36(7):78-82. [9] 欧贇,乔燕燕,王维有,等.螺旋藻抗氧化肽的制备及其体外活性研究[J].食品与生物技术学报,2014,33(1):22-26. [10] 石定燧,熊军功,陈学迅,等.高产优质饲料作物-鲁梅克斯K-1[J].新疆农业大学学报,1996,19(4):54-56. [11] 赫兰保,徐永清,李凤兰,等.盐胁迫对鲁梅克斯杂交酸模种子萌发及幼苗生理特性的影响[J].草业科学,2015(3):400-405. [12] 王峰.紫花苜蓿叶蛋白四种提取工艺比较研究[D].北京:北京林业大学,2005:14-15. [13] 王忠英.茶叶中蛋白的提取及理化性质的研究[D].杭州:浙江工商大学,2006:51-54. [14] Yen G C,Chen,H Y.Antioxidant activity of various tea extracts in relation to their antimutagenicity[J].Journal of Agricultural and Food Chemistry,1995,43(1):27-32.
[15] 乔璐.桑叶蛋白的提取与应用研究[D].金华:浙江师范大学,2014:47-48. [16] 李斌,李元甦,孟宪军,等.北五味子藤茎三萜抗氧化活性研究[J].食品工业科技,2002,33(3):122-123,128. [17] 王莉娟.大豆肽的制备及体内外抗氧化活性研究[D].无锡:江南大学,2008:10. [18] 姜传京.红萍叶蛋白提取及营养价值评价研究[D].福州:福建农林大学,2011:46-48. [19] 吕巧枝.甘薯叶可溶性蛋白的提取工艺及功能特性研究[D].北京:中国农业科学院,2007:33-35. [20] 荣建华,李小定,谢笔钧.大豆肽体外抗氧化效果的研究[J].食品科学,2002,23(11):118-120.
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