HPLC-ELSD法测定三种植物基原金线莲的金线莲苷含量

张超; 吴建国; 易骏; 吴锦忠; 郑承剑; 吴岩斌

doi:10.13386/j.issn1002-0306.2017.02.006

HPLC-ELSD法测定三种植物基原金线莲的金线莲苷含量

1. 福建中医药大学中西医结合研究院
2. 福建教育学院理科部
3. 第二军医大学药学院生药教研室

基金项目:

国家自然科学基金资助项目(81603230)；福州市科学技术局市校合作项目(2014-G-61)；福建中医药大学校管-重点学科专项(X2014138学科)；

详细信息

作者简介:
张超(1991-),男,硕士研究生,研究方向:中药活性成分及品质评价研究,E-mail:zzcc4911@163.com。;

吴岩斌(1982-),男,硕士,助理研究员,研究方向:中药资源品质评价,E-mail:wxsq1@163.com。;

中图分类号: R284.1;O657
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出版历程
- 收稿日期: 2016-07-04

Content determination of kinsenoside in Jin-Xian-Lian from three Anoectochilus species by HPLC-ELSD

Academy of Integrative Medicine,Fujian University of Traditional Chinese Medicine
Department of Chemistry and Life Science,Fujian institute of education
Department of Pharmacognosy,School of Pharmacy,Second Military Medical University

摘要

摘要: 建立HPLC-ELSD法,测定三种植物基原金线莲中金线莲苷含量。采用phenomenex NH2色谱柱（250 mm×4.6 mm,5μm）,以乙腈-水（92∶8,V/V）为流动相进行等度洗脱,流速为0.8 m L/min,柱温室温,ELSD雾化室温度为70℃,氮气流速为1.5 L/min。实验结果表明,金线莲苷进样量在76.02²432.64μg/m L范围内线性良好（r=0.9991）;精密度、稳定性、重复性实验的RSD均≤3%;平均加样回收率为97.55%,RSD为1.50%（n=6）。33批次金线莲的金线莲苷含量在6.37%²2.66%之间,平均含量为15.00%,存在较大的差异（RSD=26.29%）。该方法快速、简便,结果准确、可靠,适用于金线莲中金线莲苷的定量分析。
- 金线莲 /
- 金线莲苷 /
- HPLC-ELSD
Abstract: To establish a HPLC- ELSD method for determination of kinsenoside in Jin- Xian- Lian from three Anoectochilus species,the HPLC system consisting of phenomenex NH2column( 250 mm × 4.6 mm,5 μm) and an isocratic elution system of acetonitrile- water( 92∶ 8,V / V) as the mobile phase was adopted.The flow rate was set at 0.8 m L / min,the column temperature was room temperature,ELSD spray chamber temperature was 70 ℃,and the nitrogen flow rate was 1.5 L / min. The results indicated that the liner range of kinsenoside was 76.02 ~ 2432.64 μg / m L( r = 0.9991); RSD of precision,stability and reproducibility tests were no more than 3%; average recovery rate was 97.55%( RSD = 1.50%,n = 6). The contents of kinsenoside in Jin- Xian- Lian from three Anoectochilus species were in the range of 6.37% ~22.66%,and the average content was 15.00%( RSD = 26.29%). This method was quick,simple,accurate and reliable,and could be used to determine the content of kinsenoside in Jin- Xian- Lian.
- Jin-Xian-Lian /
- kinsenoside /
- HPLC-ELSD

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参考文献(17)

[1]	黄有霖.福建中药材标准[S].福州:海风出版社,2006:154.
[2]	中国科学院中国植物志编辑委员会.中国植物志[M].第17卷.北京:科学出版社,2000:204.
[3]	国家中医药管理局.中华本草[M].第二十四卷.上海:上海科学技术出版社,1999:672.
[4]	王国强.全国中草药汇编[M].卷二.北京:人民卫生出版社,2014:656-657.
[5]	张铁,万京,沐建华.文山地区金线莲种质资源初步调查[J].文山师范高等专科学校学报,2005,18(1):26-28.
[6]	张铁,李洪超,刘伟.文山野生金线莲的RAPD鉴别[J].中药材,2009,32(3):347-348.
[7]	郑成凤,潘裕添,蔡文燕,等.HPLC-UV-MS/MS法对金线莲中黄酮类组分的鉴定和测定[J].天然产物研究与开发,2013,25(10):1381-1386.
[8]	刘知远,沈廷明,吴仲玉.RP-HPLC法同时测定福建产金线莲中槲皮素、山柰素、异鼠李素的量[J].中草药,2015,46(3):432-434.
[9]	黄丽萍,黄丽英,林守二,等.液-质联用分析金线莲中4种黄酮类化合物[J].福建医科大学学报,2015,49(2):72-77.
[10]	关琛,王春兰,郭顺星,等.高效液相色谱法测定金线莲中黄酮含量[J].药物分析杂志,2008,28(1):9-11.
[11]	Zhang Y,Cai J,Ruan H,et al.Antihyperglycemic activity of kinsenoside,a high yielding constituent from Anoectochilus roxburghii in streptozotocin diabetic rats[J].J Ethnopharmacol,2007,114(2):141-145.
[12]	Hsiao HB,Hsieh CC,Wu JB,et al.Kinsenoside inhibits the inflammatory mediator release in a type-II collagen induced arthritis mouse model by regulating the T cells responses[J].BMC Complement Altern Med,2016,16(1):1-12.
[13]	Hsieh WT,Tsai CT,Wu JB,et al.Kinsenoside,a high yielding constituent from Anoectochilus formosanus,inhibits carbon tetrachloride induced Kupffer cells mediated liver damage[J].J Ethnopharmacol,2011,135(2):440-449.
[14]	Hsiao HB,Lin H,Wu JB,et al.Kinsenoside prevents ovariectomy-induced bone loss and suppresses osteoclastogenesis by regulating classical NF-κB pathways[J].Osteoporos Int,2013,24(5):1663-1676.
[15]	Cheng KT,Wang YS,Chou HC,et al.Kinsenoside-mediated lipolysis through an AMPK-dependent pathway in C3H10T1/2adipocytes:Roles of AMPK and PPARαin the lipolytic effect of kinsenoside[J].Phytomedicine,2015,22(6):641-647.
[16]	张锦文,唐菲,张小琼,等.高效液相色谱法测定金线莲中金线莲苷的含量[J].中国医院药学杂志,2011,31(4):261-263.
[17]	万婷,熊富良.HPLC-ELSD法测定金线莲提取物中金线莲苷[J].中成药,2014,36(7):1484-1488.