Analysis and identification of major carotenoids from Scytosiphon Lomentaria
-
摘要: 采用有机溶剂浸提法从萱藻中提取类胡萝卜素,利用柱层析与薄层层析对萱藻主要类胡萝卜素组分进行了分离纯化。利用可见吸收光谱、红外光谱与高效液相-质谱联用等检测手段对萱藻中主要类胡萝卜素组分进行了分析鉴定,并通过高效液相法对萱藻中类胡萝卜素含量进行了测定。结果表明,萱藻中主要类胡萝卜素为β-胡萝卜素和岩藻黄素,其含量分别为124.55、2862.20μg/g（dw）。Abstract: Carotenoids from Scytosiphon Lomentaria were extracted by organic solvent method in this paper. Carotenoids were separated and purified by column and thin- layer chromatography. The major carotenoids from Scytosiphon Lomentaria were analyzed and identified by visible spectrum,infrared spectroscopy and HPLC- MS. The contents of major carotenoids from Scytosiphon Lomentaria were determined by HPLC method. The results showed that the major carotenoids from Scytosiphon Lomentaria were β- carotene and fucoxanthin,which contents were 124.55 μg / g( dw) and 2862.20 μg / g( dw),respectively.
-
Keywords:
- Scytosiphon Lomentaria /
- carotenoids /
- structural identification /
- content
-
[1] 许小娟.萱藻(Scytosiphon Lomentaria)的化学成分及其生物活性研究[D].青岛:中国海洋大学,2014:16-17. [2] Fraser P D,Bramley P M.The biosynthesis and nutritional uses of carotenoids[J].Progress in Lipid Research,2004,43(3):228-265.
[3] Ladislav F,Vera P,Karel S,et al.Reliability of carotenoid analyses:a review[J].Current Analytical Chemistry,2005,1(1):93-102.
[4] YE Z W,JIANG J G,WU G H,et al.Biosynthesis and regulation of carotenoids in Dunaliella:progresses and prospects[J].Biotechnology Advances,2008,26(4):352-360.
[5] LU S,LI L.Carotenoid metabolism:biosynthesis,regulation,and beyond[J].Journal of Integrative Plant Biology,2008,50(7):778-785.
[6] Walter M H,Strack D.Carotenoids and their cleavage products:biosynthesis and functions[J].Natural Product Reports,2011,28(4):663-692.
[7] Sachindra N M,Sato E,Maeda H,et al.Radical scavenging and singlet oxygen quenching activity of marine carotenoid fucoxanthin and its metabolites[J].Journal of Agricultural and Food Chemistry,2007,55(21):8516-8522.
[8] Kanofsky J,Sima P.Quenching of Singlet Oxygen by a Carotenoid-Cyelodextrin Complex:The Importance of Aggregate Formation[J].Photochemistry and Photobiology,2008,85(1):391-399.
[9] Hayato M,Masashi H,Tokutake S,et al.Fucoxanthin from edible seaweed,Undaria pinnatifida,shows antiobesity effect through UCPI expression in white adipose tessues[J].Biophysical Research Communications,2005:332:392-397.
[10] Kotakenara E,Terasaki M,Nagao A.Characterization of apoptosis induced by fucoxanthin in human promyelocytic leukemia cells[J].Bioscience Biotechnology&Biochemistry,2005,69(1):224-227.
[11] Konishi I,Hosokawa M,Sashima T,et al.Halocynthiaxanthin and fucoxanthinol isolated from Halocynthia roretzi induce apoptosis in human leukemia,breast and colon cancer cells[J].Comparative Biochemistry and Physiology Toxicology&Pharmacology:CBP,2006,142:53-59.
[12] Kang M C,Lee S H,Lee W W,et al.Protective effect of fucoxanthin isolated from Ishige okamurae against high-glucose induced oxidative stress in human umbilical vein endothelial cells and zebrafish model[J].Journal of Functional Foods,2014(11):304-312.
[13] 任丹丹.菹草类胡萝卜素的结构鉴定、分类及生物活性的研究[D].武汉:华中农业大学,2006:13-14. [14] 任丹丹,陈倩,秦振中,等.不同处理方式对海带类胡萝卜素含量的影响[J].水产科学,2011,30(11):273-276. [15] Britton G,Liaaen-Jensen S,Pfander H,et al.Carotenoids[M].Basel:Birkllauser Verlag,1995:14-56.
[16] 张晓彤,云自厚.液相色谱检测方法[M].北京:化学工业出版社,2000:53-56. [17] 惠伯棣.类胡萝卜素化学及生物化学[M].北京:中国轻工业出版社,2005:148-174,183. [18] Islam K M S,Schweigert F J.Comparison of three spectrophotometric methods for analysis of egg yolk carotenoids[J].Food Chemistry,2015,172:233-237.
[19] Raluca M P,Yannick W,Carmen S,et al.Carotenoid composition of berries and leaves from six Romanian sea buckthorn(Hippophae rhamnoides L.)varieties[J].Food Chemistry,2014,147:1-9.
[20] Sladjana S,Gordana C,Jasna C B,et al.Tomato waste:Carotenoids content,antioxidant and cell growth activities[J].Food Chemistry,2015,172:225-232.
计量
- 文章访问数: 178
- HTML全文浏览量: 34
- PDF下载量: 334
下载:
