Optimization of ultrasonic extraction process of total saponins from Polygonatum odoratum by response surface methodology
-
摘要: 本研究在单因素实验的基础上,以菝葜皂苷元为对照品,通过响应曲面法对影响玉竹总皂苷得率的不同因素进行优化,以期研究超声波提取玉竹总皂苷的工艺条件。超声波提取的最佳工艺条件为:超声功率354 W,超声时间31 min,料液比1∶27(g∶mL)。实验结果所得玉竹总皂苷得率为4.72%,与预测值高度拟合。该法为进一步开发利用玉竹资源以及新药研发提供科学稳定的依据。Abstract: Based on the single-factor experiments,taking sarsasapogenin as the standard,response surface methodology was employed to optimize the different parameters,which affected the extraction rate of total saponins,and the technology conditions for extraction of total saponins from Polygonatum odoratum were determined. The optimum ultrasonic-assisted extraction conditions were as follows: ultrasonic power of 354 W,ultrasonic time of 31 min and solid-liquid ratio of 1∶ 27( g∶mL).Under these optimized conditions,the extraction rate of total saponins reached 4.72%,which was in good agreement with the predicted value.The method proposed in this study provided a scientific and stable base for further development and utilization of Polygonatum odoratum source as well as new drugs.
-
Keywords:
- Polygonatum odoratum /
- total saponins /
- ultrasonic /
- response surface /
- Sarsasapogenin
-
[1] Choi S B,Park S.A steroidal glycoside from Polygonatum odoratum(Mill)Druce.improves insulin resistance but does not alter insulin secretion in 90%pancreat ectomized rats[J].Biosci Biotechnol Biochem,2002,66(10):2036-2043.
[2] Lan G H,Chen H X,Wang Z S,et al.Extraction of Polygonatum odoratum polysaccharides using response surface methodology and preparation of a compound beverage[J].Carbohydrate Polymers,2011,86(3):1175-1180.
[3] 陈地灵,徐大量,林辉.玉竹总黄酮体内外抗氧化作用的实验研究[J].今日药学,2008,18(6):13-14. [4] 刘塔斯,杨先国,龚力民,等.药食两用中药玉竹的研究进展[J].中南药学,2008,6(2):216-219. [5] 郭焕杰.玉竹甾体皂苷成分及其活性研究[D].济南:济南大学,2013. [6] 王晓林,李珍,钟方丽.大孔树脂法纯化玉竹总皂苷的工艺研究[J].食品科学,2012,33(18):83-87. [7] 王俊,杨克迪,陈均.分光光度法测定薯蓣皂苷元[J].分析实验室,2004,23(1):73-75. [8] Ambati P,Ayyanna C.Optimizing medium constituentsand fermentation conditions for citric scid production from palmyra jaggery using response surface method[J].World Journal of Microbiology and Biotechnology,2001,17:331-335.
[9] Annadurai G.Design of optimum response surface experiments for adsorption of direct dye on chitosan[J].Bioprocess Engineering,2000,23:451-455.
[10] Ester R G,Alvaro B N,Alberto C B J.Optimization of medium composition for clavulanic acid production by Streptomyces clavuligerus[J].Biotechnology Letters,2001,23:157-161.
[11] Li M,Wang Z C,Dai H J,et al.Response surface optimization of polysaccharides extraction from Liriope Roots and its modulatory effect on sjogren syndrome[J].Carbohydrate Polymers,2009,45(3):284-288.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
