Optimization of extraction process for polyphenols from pineapple leaves fiber by response surface methodology
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摘要: 研究乙醇回流法提取菠萝叶纤维中总多酚,通过响应面法优化工艺条件。在单因素实验的基础上,采用响应面实验设计优化菠萝叶纤维多酚的提取工艺,建立了该工艺的二次多项数学模型,考察乙醇体积分数、料液比、提取温度、提取时间四个因素及其交互作用对菠萝叶纤维多酚提取率的影响。实验结果表明,曲面回归方程拟合性良好,其中乙醇体积分数与提取温度的交互作用显著,在乙醇体积分数59%、料液比为1∶23、提取温度71℃、提取时间118 min的条件下,验证优化工艺得到菠萝叶纤维中多酚提取率为(1.67±0.02)mg/g,与预测值1.6978 mg/g相近。Abstract: The extraction technology of total polyphenols from pineapple leaf fiber were investigated by using ethanol reflux method.Based on the single factor experimental analysis,through the response surfac,extraction conditions was optimized by investigating the effect of ethanol concentration,solid-to-solvent ratio,extraction temperature,extraction time and their interaction on thepolyphenols extracts and the process of quadric polynomial mathematics model was established. Experiment results showed that regression equation fit well with experimental data and the interaction effect of ethanol concentration and extraction temperature was significant. The optimal of extraction conditions including ethanol concentration,solid-to-solvent ratio,extraction temperature,extraction time were 59%,1 ∶23( g/m L),71 ℃ and 118 min respectively. The content of polyphenols extracted from pineapple leaf fiber under the conditions was( 1.67±0.02) mg/g closing to predicted extraction rate1.6978 mg/g.
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