静电纺丝法制备小麦醇溶蛋白纳米纤维及条件优化

袁尔东; 汪玉洁; 李秋红; 韦庆益; 任娇艳; 刘金钏; 宁正祥

doi:10.13386/j.issn1002-0306.2017.01.009

静电纺丝法制备小麦醇溶蛋白纳米纤维及条件优化

1. 华南理工大学食品科学与工程学院
2. 宜春学院

基金项目:

国家自然科学基金资助项目(31371743,21002034)；

详细信息

作者简介:
袁尔东(1975-),女,博士,副教授,研究方向:食品生物化学、食品营养与健康,E-mail:165608058@qq.com。;

中图分类号: TQ340.64;TS201.21
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出版历程
- 收稿日期: 2016-07-13

Preparation and condition optimization of electrospun Gliadin nanofibers

School of Food Sciences and Engineering,South China University of Technology
Yichun University

摘要

摘要: 通过静电纺丝法制备小麦醇溶蛋白(Gliadin)纳米纤维,采用响应面法对其工艺条件进行优化。选取小麦醇溶蛋白质量分数、电压和纺丝速度3个主要因素作为影响因子,以小麦醇溶蛋白纤维直径作为指标,通过回归分析建立多项式响应面模型。结果表明:纺丝速度对纤维直径的影响最为显著,其次是电压和质量分数。采用模拟退火算法获得最优条件为:蛋白浓度24%、电压10 kV、纺丝速度0.25 mL/h,预测纤维直径为122 nm,实际实验获得纤维直径为131 nm,说明模型预测的纤维直径与真实值能较好的拟合。采用响应面法设计,不仅简化了实验设计,可根据建立的模型设计出所需结果的实验条件,对利用静电纺丝技术制备纳米纤维具有重要的意义。
- 静电纺丝 /
- 小麦醇溶蛋白 /
- 纳米纤维 /
- 响应面实验设计
Abstract: The Gliadin nanofibers were prepared by electrospinning.Response surface method was applied to evaluate and optimize Gliadin concentration,voltage and flow rate on diameter of the electrospun nanofibers. The multiple regression model was set up by regression analysis.The results indicated that the flow rate had the mostsignificant influence,and the next were the electric field intensity and Gliadin concentration. Simulated Annealing algorithm was used to obtain the optimal conditions,which were Gliadin concentration of 24%,voltage of 10 kV,flow rate of 0.25 mL/h.Under these conditions,the predicted fiber diameter was 122 nm,the actual experimentally obtained fiber diameter was 131 nm.The observed responses were in close agreement with the predicted values of the model. By using of response surface design,not only the process of experimentat design can be simplified but also the condition of the desired fiber diameter could be obtained easily according to the model.It shows great significance on the production of electrospinning nanofibers.
- electrospin /
- Gliadin /
- nanofiber /
- response surface methodsdesign

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