Advancement of rapid detection technology for Vibrio parahaemolyticus
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摘要: 副溶血弧菌是一种重要的嗜盐性食源性致病菌,广泛存在于近海岸的海水、海底沉积物以及鱼虾、贝类等海洋生物中。在我国沿海地区,由副溶血弧菌引起的食物中毒已成为微生物中毒的首要因素。本文分别以副溶血弧菌菌体、核酸、蛋白质和代谢产物为检测对象,介绍了各种PCR技术、酶联免疫吸附技术和生物传感器技术等目前副溶血弧菌的热点快速检测技术,对各种技术进行了比较分析,并对未来的发展前景作了展望。Abstract: Vibrio parahaemolyticus is one of the most important foodborne pathogens,which is widely distributed in coastal waters,submarine sediment and marine life including fish,crustaceans and molluscsis.In the coastal areas of China,the food poisoning caused by V.parahaemolyticus have become primary microbial poisoning reasons.In this paper,many popular rapid detection technologies of V. parahaemolyticus,such as various PCR methods,ELISA and Biosensor techniques,were introduced based on cells,nucleic acid,protein and metabolites of V.parahaemolyticus as detection objects. These techniques were compared and the prospective development trends were prospected.
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[1] Bisha B,Simonson J,Janes M,et al.A review of the current status of cultural and rapid detection of Vibrio parahaemolyticus[J].International Journal of Food Science&Technology,2012,47(5):885-899.
[2] 许如苏,林彩华,黄桂荣,等.副溶血性弧菌快速测试片检测性能研究[J].中国卫生检验杂志,2009,18(12):2621-2622. [3] Peng Y,Jin Y,Lin H,et al.Application of the VPp1bacteriophage combined with a coupled enzyme system in the rapid detection of Vibrio parahaemolyticus[J].Journal of Microbiological Methods,2014,98:99-104.
[4] 纪懿芳,胡文忠,姜爱丽,等.海产品中副溶血弧菌检测方法研究进展[J].食品工业科技,2015,36(5):365-369. [5] 喻文娟,施春雷,刘玉敏,等.副溶血弧菌的热激亚致死损伤与显微红外光谱检测[J].分析化学,2013(10):1470-1476. [6] Tada J,Ohashi T,Nishimura N,et al.Detection of the thermostable direct hemolysin gene(tdh)and the thermostable direct hemolysin-related hemolysin gene(trh)of Vibrio parahaemolyticus by polymerase chain reaction[J].Molecular and Cellular probes,1992,6(6):477-487.
[7] 慕艳娟,许崛琼,潘子豪,等.以toxR-S为靶基因的副溶血弧菌PCR检测方法的建立[J].中国兽医科学,2015(10):991-999. [8] Venkateswaran K,Dohmoto N,Harayama S.Cloning and Nucleotide Sequence of the gyr B Gene of Vibrio parahaemolyticus and Its Application in Detection of This Pathogen in Shrimp[J].Applied and Environmental Microbiology,1998,64(2):681-687.
[9] 强世龙,张公亮,孙黎明,等.双重PCR快速检测海水贝类中副溶血性弧菌[J].大连工业大学学报,2015,34(1):6-10. [10] Shima T,Isobe J,Kimata K,et al.Evaluation of a Rapid Test for Determination of Most Probable Number of Viable Vibrio parahaemolyticus by Using Etidium Monoazide[J].Japanese Journal of Food Microbiology,2011,28(1):21-28.
[11] Malcolm TTH,Cheah YK,Radzi CWJWM,et al.Detection and quantification of pathogenic Vibrio parahaemolyticus in shellfish by using multiplex PCR and loop-mediated isothermal amplification assay[J].Food Control,2015,47:664-671.
[12] Izumiya H,Matsumoto K,Yahiro S,et al.Multiplex PCR assay for identification of three major pathogenic Vibrio spp.,Vibrio cholerae,Vibrio parahaemolyticus,and Vibrio vulnificus[J].Molecular and Cellular Probes,2011,25(4):174-176.
[13] Garrido A,Chapela M-J,Ferreira M,et al.Development of a multiplex real-time PCR method for pathogenic Vibrio parahaemolyticus detection(tdh+and trh+)[J].Food Control,2012,24(1):128-135.
[14] Robert-Pillot A,Copin S,Gay M,et al.Total and pathogenic Vibrio parahaemolyticus in shrimp:Fast and reliable quantification by real-time PCR[J].International Journal of Food Microbiology,2010,143(3):190-197.
[15] 林雪,陈泽辉,翁琴云,等.应用多重实时荧光PCR方法检测副溶血性弧菌及其毒力基因[J].中国卫生检验杂志,2015(6):870-872. [16] 朱耀磊,桑雪,甘未祺,等.H-NS基因作为靶基因的荧光定量PCR快速检测海产品中的副溶血性弧菌[J].食品工业科技,2015(19):155-158,162. [17] 张德福,樊晓琳,付绪磊,等.巢式PCR快速检测海产品中的副溶血弧菌[J].现代食品科技,2015(11):1-8. [18] Blanco-Abad V,Ansede-Bermejo J,Rodriguez-Castro A,et al.Evaluation of different procedures for the optimized detection of Vibrio parahaemolyticus in mussels and environmental samples[J].International Journal of Food Microbiology,2009,129(3):229-236.
[19] Nordstrom JL,Vickery MC,Blackstone GM,et al.Development of a multiplex real-time PCR assay with an internal amplification control for the detection of total and pathogenic Vibrio parahaemolyticus bacteria in oysters[J].Applied and Environmental Microbiology,2007,73(18):5840-5847.
[20] Bej AK,Patterson DP,Brasher CW,et al.Detection of total and hemolysin-producing Vibrio parahaemolyticus in shellfish using multiplex PCR amplification of tl,tdh and trh[J].Journal of Microbiological Methods,1999,36(3):215-225.
[21] Matsumoto C,Okuda J,Ishibashi M,et al.Pandemic spread of an O3:K6 clone of Vibrio parahaemolyticus and emergence of related strains evidenced by arbitrarily primed PCR and toxRS sequence analyses[J].Journal of Clinical Microbiology,2000,38(2):578-585.
[22] Wang R,Huang J,Zhang W,et al.Detection and identification of Vibrio parahaemolyticus by multiplex PCR and DNA-DNA hybridization on a microarray[J].Journal of Genetics and Genomics,2011,38(3):129-135.
[23] Lee C-Y,Pan S-F,Chen C-H.Sequence of a cloned pR72H fragment and its use for detection of Vibrio parahaemolyticus in shellfish with the PCR[J].Applied and Environmental Microbiology,1995,61(4):1311-1317.
[24] Letchumanan V,Chan K-G,Lee L-H.Vibrio parahaemolyticus:a review on the pathogenesis,prevalence,and advance molecular identification techniques[J].Front Microbiol,2014,5(705):1-13.
[25] 姜侃,张慧,汪新,等.多重LAMP—熔解曲线法检测食品中两种食源性致病菌[J].食品与机械,2015(2):87-92. [26] 罗茗月,熊礼宽.恒温扩增技术在病原体检测中的应用[J].国际检验医学杂志,2015,36(7):972-976. [27] 黄枝妙,翁育伟.环介导等温扩增技术及其在病原生物检测中的应用[J].中国人兽共患病学报,2015,31(11):1075-1080. [28] 戴婷婷,陆辰晨,郑小波.环介导等温扩增技术在病原物检测上的应用研究进展[J].南京农业大学学报,2015,38(5):695-703. [29] 纪懿芳,胡文忠,姜爱丽,等.海产品中副溶血弧菌的LAMP-HNB快速检测技术[J].食品与发酵工业,2015,41(7):142-148. [30] Prompamorn P,Sithigorngul P,Rukpratanporn S,et al.The development of loop-mediated isothermal amplification combined with lateral flow dipstick for detection of Vibrio parahaemolyticus[J].Letters in Applied Microbiology,2011,52(4):344-351.
[31] Chen W,Yu S,Zhang C,et al.Development of a single base extension-tag microarray for the detection of pathogenic Vibrio species in seafood[J].Applied Microbiology and Biotechnology,2011,89(6):1979-1990.
[32] 廖亮,刘宏生.海产品中副溶血弧菌变性高效液相色谱检测技术研究[J].中国卫生工程学,2010(3):220-222. [33] Zhan X,Zheng Q,Fu J,et al.A Rapid Multiplex PCRDHPLC Method of Detection and Identification of Pathogenic Bacteria in Aquatic Products[J].Journal of Food Safety,2015,35(1):50-58.
[34] Honda T,Yoh M,Kongmuang U,et al.Enzyme-linked immunosorbent assays for detection of thermostable direct hemolysin of Vibrio parahaemolyticus[J].Journal of Clinical Microbiology,1985,22(3):383-386.
[35] 闫茂仓,胡伟丽,张赛乐,等.基于卵黄抗体的副溶血弧菌间接ELISA快速检测方法的建立[J].山东畜牧兽医,2015(3):1-2. [36] 窦勇,胡佩红,顾鹏程.DAS-ELISA法快速检测食品中副溶血弧菌TDH毒素[J].基因组学与应用生物学,2015,34(10):2169-2175. [37] Di Pinto A,Terio V,Di Pinto P,et al.Detection of Vibrio parahaemolyticus in shellfish using polymerase chain reactionenzyme-linked immunosorbent assay[J].Letters in Applied Microbiology,2012,54(6):494-498.
[38] 周丽岩,吕宝新,任勃儒,等.胶体金免疫层析技术在食品安全检测中的应用研究[J].中国调味品,2015,40(2):128-131. [39] 杨靖亚,陆晓帆,王珍.检测副溶血弧菌TDH斑点免疫胶体金渗滤法的研究[J].中国免疫学杂志,2012,28(8):733-736. [40] 孔繁德,刘阳,徐淑菲,等.免疫金层析技术快速检测副溶血弧菌方法的初步研究[J].中国兽医科学,2012(8):819-824. [41] 林长青.上转发光技术及其在食品中病原微生物检测的应用[J].食品安全导刊,2015(10):38-41. [42] 李春凤,赵勇,王晓英,等.基于上转发光免疫层析技术的常见食源性致病菌快速检测方法研究与评价[J].军事医学,2015(2):128-132. [43] Velusamy V,Arshak K,Korostynska O,et al.An overview of foodborne pathogen detection:In the perspective of biosensors[J].Biotechnology Advances,2010,28(2):232-254.
[44] 张捷,李兆杰,王煜,等.基于分子马达生物传感器技术的副溶血性弧菌分子分型方法的初步研究[J].微生物学通报,2013,40(7):1279-1289. [45] Zhao G,Xing F,Deng S.A disposable amperometric enzyme immunosensor for rapid detection of Vibrio parahaemolyticus in food based on agarose/Nano-Au membrane and screen-printed electrode[J].Electrochemistry Communications.2007,9(6):1263-1268.
[46] Wu S,Wang Y,Duan N,et al.Colorimetric Aptasensor Based on Enzyme for the Detection of Vibrio parahemolyticus[J].Journal of Agricultural and Food Chemistry,2015,63(35):7849-7854.
[47] Sha Y,Zhang X,Li W,et al.A label-free multifunctionalized graphene oxide based electrochemiluminscence immunosensor for ultrasensitive and rapid detection of Vibrio parahaemolyticus in seawater and seafood[J].Talanta,2016,147:220-225.
[48] 操庆国,郭钦,刘涵,等.三种食源性致病菌挥发性代谢产物谱快速检测研究[J].食品工业科技,2014,35(23):290-297. [49] 黄建锋,赵广英,窦文超.智舌快速检测副溶血弧菌簇类独立软模式识别的建立[J].微生物学报,2011(4):538-546. [50] 张捷,杨向莹,许美玲,等.基于低噪声激发式荧光标记的副溶血弧菌免疫层析试纸条:中国,CN104483480A[P].2015-04-01.
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