Optimization of double enzymatic nobiletin extraction by response surface methodology
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摘要: 以柑橘皮为原料,建立紫外分光光度测定方法,研究响应面优化双酶法提取川陈皮素的最佳工艺。利用紫外分光光度法测定柑橘皮中川陈皮素的含量,在单因素实验基础上,探究纤维素酶和果胶酶复配比例、复合酶添加量、水浴温度、酶解时间、乙醇体积分数、料液比对川陈皮素得率的影响,并设计响应面分析优化方法。结果表明影响川陈皮素得率的强弱顺序为:乙醇体积分数>水浴温度>酶解时间>料液比。响应面优化后最佳工艺条件为纤维素酶和果胶酶复配比例1.5∶1、复合酶添加量5%、乙醇体积分数70%、水浴温度61℃、酶解时间2 h、料液比1∶15,所得川陈皮素得率为265.1μg/g。与响应面预测值相比,相对误差为2.3%,说明优化后得到的提取参数准确可靠,是一种适合于柑橘皮中川陈皮素提取的快速、高效方法。Abstract: Citrus skin as raw materials,establishs the method UV spectrophotometric to study the best technique that nobiletin extracted by response surface double enzymatic. By UV spectrophotometry content of citrus peel Nobiletin,and on the basis of one factor test,explore cellulase and pectinase complex ratio of enzyme dosage,Water bath temperature,reaction time,concentration of ethanol,liquid ratio on nobiletin yield,response surface analysis and design optimization methods. The results show that the impact nobiletin yield strength of the order:ethanol concentration > water bath temperature > enzymolysis time > the material- liquid ratio. After the response surface optimization optimal conditions for cellulase and pectinase complex ratio of 1.5 ∶ 1,the amount of enzyme added 5%,the ethanol concentration was 70%,Water bath temperature was 61 ℃,the enzymolysis temperature time was 2 h,the material- liquid ratio was 1 ∶ 15,resulting nobiletin yield 265.1 μg / g. Compared with the predicted value of response surface,the proof test gained the free oil yield was 2.3%,which indicates that the extraction parameters optimized to give accurate and reliable,was suitable for citrus peel extract nobiletin fast and efficient way.
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Keywords:
- double enzyme method /
- nobiletin /
- response surface method /
- extraction
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[1] 单杨.中国柑橘工业的现状、发展趋势与对策[J].中国食品学报,2008,8(1):1-8. [2] 刘晓军.柑橘加工副产品加工利用[J].农产品加工,2007(11):29-30. [3] Dhiman A,Nanda A,Ahmad S,et al.In vitro antimicrobial status of methanolic extract of Citrus sinensis Linn.fruit peel[J].Chronicles of Young Scientists,2012.
[4] Cesar T B,Aptekmann N P,Araujo M P,et al.Orange juice decreases low-density lipoprotein cholesterol in hypercholesterolemic subjects and improves lipid transfer to highdensity lipoprotein in normal and hypercholesterolemic subjects[J].Nutrition Research,2010,30(10):689-694.
[5] 单杨,李高阳,李忠海.柑橘皮中多甲氧基黄酮的体外抗氧化活性研究[J].食品科学,2007,28(8):100-103 [6] 杨武英,上官新晨,周志娥,等.柑桔皮总黄酮提取工艺的研究[J].江西农业大学学报,2009,31(2):345-349. [7] Delaney B,Phillips K,Buswell D,et al.Immunotoxicity of a standardized citrus polymethoxylated flavone extract[J].Food&Chemical Toxicology An International Journal Published for the British Industrial Biological Research Association,2001,39(11):1087-1094.
[8] 王光凤,王小晨,肖磷,等.柑橘黄酮川陈皮素的抗肿瘤作用研究[J].中草药,2007,38(11):1694-1697. [9] Ortuo A,Díaz L,Alvarez N,et al.Comparative study of flavonoid and scoparone accumulation in different Citrus species and their susceptibility to Penicillium digitatum[J].Food Chemistry,2011,125(1):232-239.
[10] 于宏伟,韩卫荣,周二鹏,等.橘皮素的提取及应用研究进展[J].江苏农业科学,2012,40(3):249-252. [11] 李成平,田红梅,金在宿,等.微波辅助提取橘皮中川陈皮素和橘红素的工艺研究[J].食品科技,2011(5):224-228. [12] 张玉,李洪军,窦华亭,等.响应面法优化超声波提取川皮苷工艺研究[J].食品科学,2012(20):123-127. [13] 徐旭耀,王汝娟,谢伟飞.柑橘皮中川陈皮素超声提取工艺及含量分析[J].食品工业科技,2012,33(9):301-304. [14] 罗世坤,王家欢,徐兰英,等.陈皮中川陈皮素的提取分离及改性[J].食品科学,2014,35(24):20-23. [15] 董文成.柑橘果实膳食纤维物化特性及其性能表征研究[D].浙江大学,2015. [16] Li B B,Smith B,Hossain M M.Extraction of phenolics from citrus peels:II.Enzyme-assisted extraction method[J].Separation&Purification Technology,2013,48(2):189-196.
[17] 陈源.金柑等柑橘类果实黄酮类化合物提取、纯化及分离鉴定[D].福建农林大学,2011. [18] Li C P,Wang L L,Jin Z S,et al.Study on the Extraction Technique of Poly-methoxyflavonoids from Citrus Peels by Using Response Surface Methodology[J].Advanced Materials Research,2012,560-561:544-549.
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