10株乳酸杆菌吸附铅离子及抗氧化能力的比较

于上富; 靳妲; 丁秀云; 徐敏; 霍贵成

doi:10.13386/j.issn1002-0306.2016.24.040

10株乳酸杆菌吸附铅离子及抗氧化能力的比较

东北农业大学食品学院,乳品科学教育部重点实验室

基金项目:

国家自然科学基金(31401512)；国家高技术研究发展计划(863计划)(2011AA100902)；

详细信息

作者简介:
于上富(1989-),男,硕士研究生,研究方向:食品微生物与生物技术,E-mail:yushangf@163.com。;

霍贵成(1958-),男,博士,教授,研究方向:食品微生物与生物技术,E-mail:gchuo58@126.com。;

中图分类号: TS201.3
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出版历程
- 收稿日期: 2016-05-23

In vitro antioxidant activity of ten strains of lactobacillus with sequestering lead ions

Key Laboratory of Dairy Science of Ministry of Education,College of Food Science,Northeast Agricultural University

摘要

摘要: 为了筛选出高吸附铅离子且具高抗氧化能力的菌株,本文对10株乳杆菌进行了铅离子吸附研究,以DPPH·清除力、还原能力、羟自由基清除及抗脂质过氧化能力为指标进行了体外抗氧化能力的评定。结果表明:不同乳杆菌之间的清除铅离子能力不同,抗氧化能力也不相同,其中德式乳杆菌保加利亚亚种KLDS1.0207具有高吸附铅离子和高抗氧化能力,该菌株铅离子去除率达79.18%,还原能力达99.00μmol/L的半胱氨酸当量,DPPH·清除率为50.40%,羟自由基清除率26.88%,抗脂质过氧化能力达15.62%。此菌株可用于体内缓解铅中毒造成的氧化损伤的研究。
- 氧化损伤 /
- 乳杆菌 /
- 抗氧化性 /
- 生物吸附
Abstract: In order to select strains of high adsorption of lead ions strains and has antioxidant capacity,the 10 strains of lactobacillus of lead ions adsorption experiment were conducted in this experiment,and the antioxidant capabilities of 10 strains of lactic acid bacteria in vitro were evaluated through reducing radical activity assay,scavenging DPPH free radicals,scavenging hydroxyl free radical and inhibiting linoleic acid peroxidation.The ability to remove lead ions between different Lactobacillus different,not the same antioxidant capacity.The results showed that KLDS 1.0207 had the capacity of sequestering lead ions was 79.18%,reducing power up to 99.00 μmol / L cysteine equivalents. Scavenging action level of DPPH and hydroxyl free radicals were 50.40%,26.88%respectively.In liposome system,anti- lipid peroxidation resistance only reached 15.62%.This strain can be used to alleviate the oxidative damage in the body caused by lead poisoning.
- oxidative damage /
- lactobacillus /
- oxidation resistance /
- biosorption

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参考文献(37)

[1]	冯国昌.排铅聚糖对铅染毒小鼠的治疗作用机理及其毒理学研究[D].济南:山东大学,2007.
[2]	Mielke H W.Research ethics in pediatric environmental health:Lessons from lead[J].Neurotoxicology&Teratology,2002,24(4):467-469.
[3]	Fewtrell L J,Prüss-üstün A,Landrigan P,et al.Estimating the global burden of disease of mild mental retardation and cardiovascular diseases from environmental lead exposure[J].Environmental Research,2004,94(2):120-133.
[4]	Sansar W,Bouyatas M M,Ahboucha S,et al.Effects of chronic lead intoxication on rat serotoninergic system and anxiety behavior[J].Acta Histochemica,2012,114(1):41-45.
[5]	Sansar W,Ahboucha S,Gamrani H.Chronic lead intoxication affects glial and neural systems and induces hypoactivity in adult rat[J].Acta Histochemica,2011,113(6):601-607.
[6]	Sabokbar N,Khodaiyan F.Total phenolic content and antioxidant activities of pomegranate juice and whey based novel beverage fermented by kefir grains[J].Journal of Food Science and Technology,2016,53(1):739-747.
[7]	Zelikoff J T,Parsons E,Schlesinger R B.Inhalation of Particulate Lead Oxide Disrupts Pulmonary Macrophage-Mediated Functions Important for Host Defense and Tumor Surveillance in the Lung[J].Environmental Research,1993,62(2):207-222.
[8]	Hosseini A,Sharifi A M,Abdollahi M,et al.Cerium and Yttrium Oxide Nanoparticles Against Lead-Induced Oxidative Stress and Apoptosis in Rat Hippocampus[J].Biological Trace Element Research,2015,164(1):80-89.
[9]	Tangpong J,Satarug S.Alleviation of lead poisoning in the brain with aqueous leaf extract of the Thunbergia laurifolia(Linn.)[J].Toxicology Letters,2010,198(1):83-88.
[10]	Jonkers D M A E.Microbial perturbations and modulation in conditions associated with malnutrition and malabsorption[J].Best Practice&Research Clinical Gastroenterology,2016,30(2):161-172.
[11]	Chapman C M C,Gibson G R,Rowland I.Health benefits of probiotics:are mixtures more effective than single strains?[J].European Journal of Nutrition,2011,50(1):1-17.
[12]	翟齐啸.乳酸菌减除镉危害的作用及机制研究[D].无锡:江南大学,2015.
[13]	田丰伟,翟齐啸,孙媛媛,等.缓解铅毒性植物乳杆菌CCFM8661的微生物学性质及其应用的研究[J].食品安全质量检测学报,2014(4):1010-1015.
[14]	Mishra V,Shah C,Mokashe N,et al.Probiotics as Potential Antioxidants:A Systematic Review[J].Journal of Agricultural and Food Chemistry,2015,63(14):3615-3626.
[15]	田丰伟.缓解氧化应激乳酸菌的筛选、表征和功能评价研究[D].无锡:江南大学,2012.
[16]	唐雅茹,于上富,国立东,等.一株降胆固醇乳杆菌的筛选及其益生作用的研究[J].食品工业科技,2016,37(1):142-144,152.
[17]	杜金城,徐敏,李柏良,等.具有抑制大肠杆菌作用的乳酸菌的初步筛选及其益生潜能的研究[J].食品工业科技,2016(13):1-8.
[18]	Halttunen T,Collado M C,El-Nezami H,et al.Combining strains of lactic acid bacteria may reduce their toxin and heavy metal removal efficiency from aqueous solution[J].Lett Appl Microbiol,2008,46(2):160-165.
[19]	Halttunen T,Salminen S,Tahvonen R.Rapid removal of lead and cadmium from water by specific lactic acid bacteria[J].International Journal of Food Microbiology,2007,114(1):30-35.
[20]	Halttunen T,Kankaanpaa P,Tahvonen R,et al.Cadmium removal by lactic acid bacteria[J].Bioscience Microflora,2003,3(22):93-97.
[21]	Bian X,Evivie S E,Muhammad Z,et al.In vitro assessment of the antimicrobial potentials of Lactobacillus helveticus strains isolated from traditional cheese in Sinkiang China against foodborne pathogens[J].Food Funct,2016,7(2):789-797.
[22]	白明,孟祥晨.益生菌抗氧化活性及菌体抗氧化相关成分的分析[J].食品与发酵工业,2009(5):6-11.
[23]	Lin M,Yen C.Antioxidative Ability of Lactic Acid Bacteria[J].Journal of Agricultural and Food Chemistry,1999,47(4):1460-1466.
[24]	Lin M Y,Chang F J.Antioxidative effect of intestinal bacteria Bifidobacterium longum ATCC 15708 and Lactobacillus acidophilus ATCC 4356[J].Dig Dis Sci,2000,45(8):1617-1622.
[25]	Zhai Q,Yin R,Yu L,et al.Screening of lactic acid bacteria with potential protective effects against cadmium toxicity[J].Food Control,2015,54:23-30.
[26]	Zhang S,Liu L,Su Y,et al.Antioxidative activity of lactic acid bacteria in yogurt[J].African Journal of Microbiology Research,2011,29(5):5194-5201.
[27]	刘洋,郭宇星,潘道东.4种乳酸菌体外抗氧化能力的比较研究[J].食品科学,2012(11):25-29.
[28]	葛伟.超声预处理对酪蛋白美拉德反应及其产物功能特性的影响[D].哈尔滨:东北农业大学,2015.
[29]	Wang Y,Yu R,Chou C.Antioxidative activities of soymilk fermented with lactic acid bacteria and bifidobacteria[J].Food Microbiology,2006,23(2):128-135.
[30]	Naik M M,Dubey S K.Lead resistant bacteria:Lead resistance mechanisms,their applications in lead bioremediation and biomonitoring[J].Ecotoxicology and Environmental Safety,2013,98:1-7.
[31]	Zoghi A,Khosravi-Darani K,Sohrabvandi S.Surface binding of toxins and heavy metals by probiotics[J].Mini Rev Med Chem,2014,14(1):84-98.
[32]	Beveridge T J,Murray R G.Sites of metal deposition in the cell wall of Bacillus subtilis[J].J Bacteriol,1980,141(2):876-887.
[33]	Naik M M,Pandey A,Dubey S K.Biological characterization of lead-enhanced exopolysaccharide produced by a lead resistant Enterobacter cloacae strain P2B[J].Biodegradation,2012,23(5):775-783.
[34]	车驰.新疆酸马奶抗氧化活性乳酸菌的筛选及其特性研究[D].乌鲁木齐:新疆农业大学,2012.
[35]	Kachouri F,Ksontini H,Kraiem M,et al.Involvement of antioxidant activity of Lactobacillus plantarum on functional properties of olive phenolic compounds[J].Journal of Food Science and Technology,2015,52(12):7924-7933.
[36]	王刚,田丰伟,刘小鸣,等.2株具有优良体外抗氧化能力乳酸菌的筛选与鉴定[J].食品工业科技,2013(15):149-153.
[37]	左玉.脂质过氧化及抗氧化剂抗氧化活性检测方法[J].粮食与油脂,2009(2):39-42.