Study on preparation process of high degree transparency decolorization squid skin collagen
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摘要: 为了提高鱿鱼皮胶原蛋白的透明度和应用价值,本文利用双氧水对鱿鱼皮胶原蛋白进行脱色工艺优化研究。酶解提取得到的鱿鱼皮胶原蛋白通过单因素实验和正交实验,以胶原蛋白回收率和胶原蛋白脱色率为指标,确定了最佳胶原蛋白回收和脱色工艺条件为双氧水浓度0.6%,p H8,温度65℃,脱色时间7 h。在最佳的蛋白回收和脱色工艺条件下,胶原蛋白的脱色率和蛋白回收率分别为78.76%和88.29%。因此,本研究所得胶原蛋白回收和脱色效果较好,具有潜在的应用前景Abstract: In order to improve transparency degree and application value of squid skin collagen,this paper studied the process on using hydrogen peroxide oxidation to decolorize the pigment of squid skin collagen.With one- factor- at- a- time method and orthogonal experiment of the squid skin collagen obtained by enzymatic hydrolysis,the optimal protein recovery and decolorization process conditions of squid skin collagen were the concentration of hydrogen peroxide of 0.6%,p H value of 8.0,temperature of 65 ℃,and the reaction time of 7 h. Under the optimal protein recovery and decolorization process conditions,the decolorization rate and protein recovery rate of squid skin collagen were 78.76% and 88.29%,respectively.So,the results of this study had better collagen recovery and decolorization effect and had potential application prospects.
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Keywords:
- squid skin /
- collagen /
- H_2O_2 solution /
- decolorization rate /
- protein extraction rate
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