Analysis of chemical components by GC-MS and scavenging capacity against DPPH free radical of SFE extracts from Trigonella foenum-graecum L.
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摘要: 采用超临界二氧化碳提取葫芦巴提取物,利用气相色谱-质谱法分析提取物的化学成分,并通过面积归一化法分析各成分的相对含量。从葫芦巴超临界二氧化碳提取物中共鉴定出49个化合物,占提取物总量的88.37%,其中亚油酸（45.29%）含量最高。利用分光光度法测定葫芦巴超临界二氧化碳提取物对DPPH自由基的清除作用,其清除作用良好,半清除浓度为4.57 mg·m L-1,且清除率与样品浓度呈量效关系。葫芦巴超临界提取物营养成分丰富、安全无毒、有特殊香气,在食品、香料等领域可广泛应用。Abstract: Supercritical fluid extraction( SFE) using carbon dioxide was employed to extract Trigonella foenum-graecum L.and the chemical components were determined by GC- MS. The relative contents of the chemical components were determined with peak area normalization method. Forty- nine compounds,which occupied88.37% of total constituents were identified. The major constituent was linoleic acid( 45.29%). The scavenging capacity against DPPH free radical of SFE extracts from Trigonella foenum- graecum L.was determined by spectro photometry.The extracts exhibited significant scavenging capacity against DPPH free radical in a concentrationdependent fashion with the SC50 value of 4.57 mg·m L- 1.The SFE extracts was rich in nutrients,safe and non- toxic,and had special aroma.So it could be widely used in the fields of food and perfume.
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