Study on the oxidative damage effects of dibutyl phthalate on Caenorhabditis elegans
-
摘要: 为了探讨塑化剂邻苯二甲酸二丁酯（DBP）对秀丽隐杆线虫的氧化损伤作用,将L2期秀丽隐杆线虫暴露于不同浓度DBP（32.5、131、522 mg/L）48 h后,检测秀丽隐杆线虫体内总超氧化物歧化酶（T-SOD）和过氧化氢酶（CAT）活力、谷胱甘肽（GSH）及丙二醛（MDA）含量的影响。实验结果表明,随着DBP浓度升高,线虫体内MDA含量升高。与对照组相比,522 mg/L DBP处理组秀丽隐杆线虫体内T-SOD和CAT酶活力、GSH含量均极显著降低（p<0.01）。结论:DBP对秀丽隐杆线虫产生氧化损伤作用。Abstract: The aim of this article was to investigate the oxidative damage effect of dibutyl phthalate( DBP) in C.elegans.C.elegans in L2 period were expoused to the different concentrations of DBP( 32.5,131 and 522 mg / L)for 48 h. Then the activities of T- SOD,CAT and the contents of GSH,MDA in C.elegans were measured. With increasing DBP concentration,the contents of MDA increased. Compared with the control group,the activities of T- SOD and CAT and the contents of GSH were significantly decreased in 522 mg / L DBP treatment group( p <0.01).These results suggested that DBP could induce the oxidative damage in C.elegans.
-
Keywords:
- Caenorhabditis elegans /
- dibutyl phthalate /
- oxidative damage
-
[1] Schettler T.Human exposure to phthalates via consumer products[J].Int J Androl,2006,29(1):134-139.
[2] Wormuth M,Scheringer M,Vollenweider M,et al.What are the sources of exposure to eight frequently used phthalic acid esters in Europeans[J].Risk Anal,2006,26(3):803-824.
[3] 王立鑫,杨旭.邻苯二甲酸酯毒性及健康效应研究进展[J].环境与健康杂志,2010,27(3):276-281.. [4] 郭永梅.邻苯二甲酸酯的毒性及相关限制法规[J].广州化学,2012,37(2):75-79. [5] 常兵,刘德瑜,梁玉香,等.邻苯二甲酸二丁酯对青春期雄性大鼠的生殖毒性研究[J].中国自然医学杂志,2007,9(3):164-168. [6] 陈洪涛.邻苯二甲酸酯毒理学研究进展[J].食品与机械,2012,28(5):251-254. [7] Tseng IL,Yang YF,Yu CW,et al.Phthalates induce neurotoxicity affecting locomotor and thermotactic behaviors and AFD neurons through oxidative stress in Caenorhabditis elegans[J].PLo S One,2013,8(12):e82657.
[8] Rankin CH.From gene to identified neuron to behaviour in Caenorhabditis elegans[J].Nat Rev Genet,2002,3(8):622-630.
[9] Leung MCK,Williams PL,Benedetto AAC,et al.Caenorhabditis elegans:an emerging model in biomedical and environmental toxicology[J].Toxicol Sci,2008,106(1):17-19.
[10] 屈长青,徐林丽,陆娟,等.罗勒水提物对秀丽隐杆线虫脂肪沉积的影响[J].中国生化药物杂志,2012,33(2):165-166. [11] 夏世钧,吴中亮.分子毒理学基础[M].武汉:湖北科学技术出版社,2001. [12] 秦洁芳,陈海刚,蔡文贵,等.邻苯二甲酸二丁酯对翡翠贻贝抗氧化酶及脂质过氧化水平的影响[J].应用生态学报,2011,22(7):1878-1884. [13] 王艳.几种邻苯二甲酸酯对蚯蚓(Eisenia fetida)的分子毒性机理[D].杭州:浙江工业大学,2013:5-10. [14] 蔡立哲,马丽,高阳,等.蒽、菲、芘、混合液对菲律宾蛤仔抗氧化酶活性的影响[J].海洋科学,2005,29(8):47-52. [15] Pan LQ,Ren J,Liu J.Responses of antioxidant systems and LPO level to benzo(a)pyrene and benzo(k)fluoranthene in the haemolymph of the scallop Chlamys ferrari[J].Environ Pollut,2006,141(3):443-451.
[16] 秦文娟.邻苯二甲酸二丁醋对雄性小鼠生殖毒性及氧化损伤作用的研究[D].武汉:华中师范大学,2011:15-20. [17] Ourique GM,Finamor IA,Saccol EM,et al.Resveratrol improves sperm motility,prevents lipid peroxidation and enhances antioxidant defences in the testes of hyperthyroid rats[J].Reproductive Toxicology,2013,37:31-39.
[18] 蔡风云,王萌,王婧,等.邻苯二甲酸二丁酯对体外大鼠肝脏细胞的氧化损伤作用[J].公共卫生与预防医学,2009,20(4):4-7. [19] Min A,Liu F,Yang X,et al.Benzyl butyl phthalate exposure impairs learning and memory and attenuates neurotransmission and CREB phosphorylation in mice[J].Food Chem Toxicol,2014,71:81-89.
[20] Yolton K,Xu Y,Strauss D,et al.Prenatal exposure to bisphenol A and phthalates and infant neurobehavior[J].Neurotoxicol Teratol,2011,33(5):558-566.
[21] Cho SC,Bhang SY,Hong YC,et al.Relationship between environmental phthalate exposure and the intelligence of schoolage children[J].Environ Health Perspect,2010,118(7):1027-1032.
计量
- 文章访问数: 158
- HTML全文浏览量: 15
- PDF下载量: 157
下载:
