15种青藏高原植物提取物对几种常见微生物的抑制作用研究

张忠; 李科玮; 毕阳; 王毅; 任亚琳

doi:10.13386/j.issn1002-0306.2016.24.014

15种青藏高原植物提取物对几种常见微生物的抑制作用研究

甘肃农业大学食品科学与工程学院

基金项目:

国家自然科学地区基金项目(31360416)；甘肃省中药材产业科技攻关项目(甘肃大宗道地中药材安全防蛀防霉技术研究)；甘肃省农业科技创新项目(GNCX-2012-42)；甘肃省高校基本科研业务费项目(2014)；

详细信息

作者简介:
张忠(1977-),男,在读博士研究生,副教授,主要从事天然抗菌活性物质方面的研究,E-mail:zhangzhong@gsau.edu.cn。;

毕阳(1962-),男,博士,教授,研究方向:采后生物学与技术,食品风味,E-mail:beyang62@163.com。;

中图分类号: TS201.3
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出版历程
- 收稿日期: 2016-05-19

Inhibition effects of extracts from 15 kinds Tibetan plateau plants against several common microorganisms

College of Food Science and Engineering,Gansu Agricultural University

摘要

摘要: 为研究青藏高原植物提取物对几种常见微生物的抑制效果,本文采用琼脂孔扩散法和96孔培养板对倍稀释法研究了15种高原植物提取物对4种真菌和4种细菌的体外抑菌率和最低抑菌浓度。结果显示:各种植物提取物具有不同的抑菌谱和最小抑制浓度,其中黄腺香青、刺柏、塞北紫芹、龙胆花、苔草的提取物至少对4种供试菌有抑制作用;苔草的三种提取物对金黄色葡萄球菌、大肠杆菌与荧光假单孢菌的最低抑菌浓度为0.05 g/m L;大部分提取物对细菌的抑制效果优于对真菌的抑制效果。结果表明生长于青藏高原独特生态条件下的许多植物,其代谢物在体外具有较强的抑菌活性和较广的抑菌谱。
- 青藏高原植物 /
- 琼脂孔扩散法 /
- 最小抑制浓度 /
- 食源性致病菌
Abstract: To investigate the antimicrobial activity of some Tibetan plateau plant extracts,agar- diffusion method and96- well plate double dilution method were used to evaluate the inhibition effects and minimum inhibitory concentration( MIC) of different extracts from 15 kinds of Tibetan plateau plant samples. The results showed that extracts from different plateau plants had different antimicrobial spectrum and MICs. Extracts from Anaphalia aureopunctata,Juniperus formosara,Corydalis impatiens,Gentiana sino- ornata and Carex tristachya can inhibited at least 4 tested microorganisms,the MIC of three extracts from Carex tristachya against Staphylococcus aureus,Escherichia coli and Bacillus subtilis was 0.05 g·m L- 1. Most of extracts showed more effective inhibition against bacteria than fungi.It concluded that several kinds extract from plateau plants had wide antimicrobial range and strong inhibition activity against common microorganisms.
- Tibetan plateau plants /
- agar-diffusion method /
- minimum inhibitory concentration /
- common microorganisms

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