Inhibitory effect of mogrosides crude extract on α-glucosidase in vitro
-
摘要: 目的:研究罗汉果皂甙粗提物对α-葡萄糖苷酶活性的抑制作用。方法:通过利用荧光光谱法和分光光度法探讨罗汉果皂甙粗提物体外抑制α-葡萄糖苷酶的效果。结果:罗汉果皂甙粗提物对α-葡萄糖苷酶具有一定的抑制作用,其IC50值为9.125 mg/m L。动力学分析及荧光光谱法分析表明,罗汉果皂甙粗提物对α-葡萄糖苷酶的抑制作用为混合型抑制,对α-葡萄糖苷酶内源性荧光产生强烈的静态猝灭作用。结论:罗汉果皂甙粗提物对α-葡萄糖苷酶具有抑制作用,提示罗汉果在降血糖产品开发方面具有较好的应用前景。
-
关键词:
- 罗汉果皂甙 /
- α-葡萄糖苷酶抑制活性 /
- 荧光猝灭 /
- 抑制动力学
Abstract: Objective: The inhibitory effect of mogrosides crude extract on the activity of α- glucosidase was studied.Methods: The fluorescence spectroscopy methods and spectrophotometry were used for analysis the inhibitory activity of mogrosides crude extract on α- glucosidase.Results: The mogrosides crude extract had inhibitory effect on α- glucosidase with 9.125 mg / m L IC50 value. The kinetic and fluorescence spectroscopy analysis showed that the inhibition effect of mogrosides crude extract on α- glucosidase belonged to mixed inhibition,which had strong static quenching effect on the intrinsic fluorescence of α- glucosidase. Conclusion: The mogrosides crude extract had inhibitory effect on the α- glucosidase activity,which indicated that mogrosides crude had good application prospects in antidiabetic product development. -
[1] 程振田,袁祥萍,张心中.糖尿病药物治疗研究进展[J].医学综述,2003,9(10):627-629. [2] 许曼音,陆广华,陈名道.糖尿病学[M].上海:上海科学技术出版社,2003:12-15. [3] 王翼,张旭.α-葡萄糖苷酶抑制剂的研究进展[J].海峡药学,2009,21(9):4-5. [4] 覃志成,柴可夫.中药治疗糖尿病机制的研究进展[J].浙江中医学院学报,2004,28(1):12-15. [5] 冯长根,陈凌,刘霞.以中草药为来源的α-葡萄糖苷酶抑制剂筛选研究进展[J].中国新药杂志,2005,14(6):669-672. [6] 张静,吴友良,张婵娟,等.罗汉果药理活性研究进展[J].中国药业,2010,19(20):84-86. [7] 陈迪华,斯建勇,常琪,等.天然甜味剂罗汉果的研究与应用[J].天然产物研究与开发,1992,4(1):72-77. [8] 尹学哲,全吉淑,金泽武道,等.大豆异黄酮和皂苷对糖尿病大鼠抗动脉粥样硬化活性的研究[J].中国预防医学杂志,2004,38(1):26-28. [9] 安家炜,张有做,高前欣,等.美国豆芋地上部位有效成分及α-葡萄糖苷酶抑制活性研究[J].核农学报,2014,28(12):2275-2282. [10] 全吉淑,尹学哲,金明,等.大豆皂苷对α-葡萄糖苷酶抑制作用的研究[J].中药材,2003,26(9):654-656. [11] 陈丽华,马庆一,杨冰.α-葡萄糖苷酶抑制剂研究进展[J].食品科技,2005,28(8):91-94. [12] 谭玉莲,王一涛,许贝文.罗汉果皂苷对大鼠小肠微血管动脉在高糖环境下内皮完整血管的舒张保护作用[J].中药药理与临床,2014,30(5):97-100. [13] 周英,郑艳,EBERSOLE Jeff,等.罗汉果提取物和罗汉果苷V对胰岛素分泌的调节作用[J].药学学报,2009,44(11):1252-1257. [14] YA Suzuki,M Yuji,I Hiroshi,et al.Triterpene glycosides of siraitia grosvenori Inhibit rat intestinal maltase and suppress the rise in blood glucose level after a single oral administration of maltose in rats[J].J Agric Food Chem,2005,53(8):2941-2946.
[15] 何伟平,朱晓韵,何超文.罗汉果的功能作用及食品应用研究进展[J].轻工科技,2012,1(1):1-3. [16] 张俐勤,戚向阳,陈维军,等.罗汉果皂苷提取物对糖尿病小鼠血糖、血脂及抗氧化作用的影响[J].中国药理学通报,2006,22(2):237-240. [17] 朱慧玲.罗汉果皂甙类化合物的分离、纯化及其抗氧化活性研究[D].武汉:华中农业大学,2014. [18] 柳余莉,杨文康,刘合生,等.杨梅多酚粗提物对α-葡萄糖苷酶体外抑制作用的研究[J].果树学报,2016,33(1):59-65. [19] YQ Li,FC Zhou,F Gao.Comparative evaluation of quercetin,isoquercetin and rutin as inhibitors ofα-glucosidase[J].Journal of Agricultural and Food Chemistry,2009,57(24):11463-11467.
[20] YAN J K,ZHANG G W,PAN J H,et al.α-Glucosidase inhibition by luteolin:Kinetics,interaction and molecular docking[J].International Journal of Macromolecules,2014,64(12):213-215.
[21] 杨荣武.生物化学原理[M].北京:高等教育出版社,2012:132-149. [22] J Tian,J Liu,X Tian,et al.Study of the interaction of kaempferol with bovine serum albumin[J].Journal of Molecular Structure,2004,691(1-3):197-202.
[23] M Yang,X Xi,P Yang.Thermodynamic analysis of fluorescence enhancement and quenching theory equations[J].Frontiers of Chemistry in China,2008,3(3):254-261.
[24] ZHANG Xiaowei,ZHAO Fenglin,LI Kean.Progress in the research of the interaction between drug and serum protein in vitro[J].Chemistry Online,2001,64(1):65.
[25] Bischoff H.The mechanism of alpha-glucosidase inhibition in the management of diabetes[J].Clinical and Investigative Medicine,1995,18(4):303-311.
[26] Casirola D M,Ferraris R P.alpha-glucosidase inhibitors prevent diet-induced increases in intestinal sugar transport in diabetic mice[J].Metabolism,2006,55(6):832-841.
[27] 陈海敏,严小军,林伟.α-葡萄糖苷酶抑制剂的构效关系[J].中国生物化学与分子生物学报,2003,19(6):780-784. [28] H Park,KY Hwang,KH Oh,et al.Discovery of novel a-glucosidase inhibitors based on the virtual screening with the homology-modeled protein structure[J].Bioorg Med Chem,2008,16(1):284-292.
[29] K Bharatham,N Bharatham,KH Park,et al.Binding mode analyses and pharmacophore model development for sulfonamide chalcone derivatives,a new class of a-glucosidase inhibitors[J].J Mol Craph Model,2008,26(8):1202-1212.
-
期刊类型引用(14)
1. 田宝明，叶芃，孔俊涛，杨开，孙培龙，陈红平，王舰，张相春. 茶多酚自组装纳米泡腾片的研制. 中国茶叶加工. 2024(02): 33-40 . 
百度学术
2. 冯思思，张喜瑞，于梦言，徐玉孟，李梓诺，李嘉慧，孙万萌，王璐，梁彬，孙婵婵. 黑蒜泡腾片的制备工艺优化及其对肠道微生态的影响. 食品与发酵工业. 2024(19): 49-55+86 . 百度学术
3. 李妍，吴少康，胡浩，张旭，张向荣. 黑果腺肋花楸泡腾片的研制. 中国药剂学杂志(网络版). 2023(01): 1-11 . 百度学术
4. 薛雅茹，陈紫红，黄茂坤. 响应面法优化马鲛鱼内脏抗氧化肽泡腾片制备工艺及其质量评价. 食品工业科技. 2023(08): 244-251 . 本站查看
5. 王媛媛，郝璟嵘，闫思颖，张甜甜，党玲，王晓婧. 沙棘冻干粉泡腾片的制备工艺优化及其品质分析. 食品工业科技. 2023(10): 235-241 . 本站查看
6. 孔庆新，东方，孙兰，徐伟平，郭锐，张荣浩，罗丽梅，刘碧林. 荔枝草治疗急性咽炎有效部位筛选. 世界科学技术-中医药现代化. 2022(02): 686-693 . 百度学术
7. 肖云. 乌梅山楂泡腾片的研制及质量评价. 武汉职业技术学院学报. 2022(03): 109-114 . 百度学术
8. 沈文，吴立新，刘树兴，延梦瑶. 蜂王浆泡腾片的制备及王浆酸溶出的测定. 食品工业科技. 2022(14): 276-282 . 本站查看
9. 黄冉涛，王聚恒，周青辉，田花丽，乔清涛. 荔枝草研究进展及产品开发概况. 农产品加工. 2022(16): 68-70+74 . 百度学术
10. 赖菁华，杨晓雯. 响应面法优化乌梅泡腾片的配方. 食品工业. 2020(04): 91-95 . 百度学术
11. 王慧竹，王禹衡，张兴涛，姜诗宇，陈帅. 罗布麻总黄酮泡腾片的制备工艺及抗氧化活性. 食品工业. 2020(08): 130-135 . 百度学术
12. 章斌，姚永秀，袁野，张恒，卢勇，彭裕红. 藏茶泡腾片的配方优化. 食品工业科技. 2020(18): 123-129 . 本站查看
13. 严利平，郭丹，练学燕，汪杨丽，李睿，李东. 红茶泡腾片制备工艺. 食品工业. 2020(12): 67-72 . 百度学术
14. 姚洪礼，邢爽，周先汉，陆宁，丁之恩，蒲顺昌. 红枣山药固体速溶泡腾片制备及特性. 食品工业. 2020(12): 77-81 . 百度学术
其他类型引用(3)
计量
- 文章访问数: 170
- HTML全文浏览量: 17
- PDF下载量: 349
- 被引次数: 17
下载:
