发酵鹅肝肽的分离纯化及其抗氧化特性研究

舒沿沿; 胡冰雪; 潘道东; 曾小群; 孙杨赢; 吴振; 曹锦轩

doi:10.13386/j.issn1002-0306.2016.24.012

发酵鹅肝肽的分离纯化及其抗氧化特性研究

宁波大学食品科学与工程系

基金项目:

水禽肉品质及其深加工技术研究(ZX2012000380)；

详细信息

作者简介:
舒沿沿(1989-),女,在读硕士研究生,研究方向:功能性因子,E-mail:shuyanyan900707@163.com。;

潘道东(1964-),男,博士,教授,研究方向:畜产品加工,E-mail:daodongpan@163.com。;

中图分类号: TS251.4
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出版历程
- 收稿日期: 2016-06-29

Isolation and antioxidant activity of fermentation peptides from goose liver

Department of Food Science and Engineering,Ningbo University

摘要

摘要: 为了提高普通鹅肝的综合利用价值,本研究利用解淀粉芽孢杆菌（Bacillus amyloliquefaciens）、地衣芽孢杆菌（Bacillus licheniformis）、嗜酸乳杆菌（Lactobacillus acidophilus）混合发酵制备鹅肝肽,采用Sephadex G-25凝胶层析法分离纯化出发酵鹅肝肽,并对其分离组分进行氨基酸分析和功能特性研究（抗氧化活性、金属螯合率测定）,最后通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF/TOF）测定高活性组分的相对分子质量。结果表明:Sephadex G-25凝胶层析依次出现P1、P2、P3三个峰;其中P3的必需氨基酸含量（70.32±5.35）mg/100 g明显高于P1（40.06±2.04）mg/100 g和P2（55.39±2.56）mg/100 g,含硫氨基酸（Met、Cys）和疏水性氨基酸（Val、Ile、Leu、Phe、Ala）分别占总氨基酸含量的17.78%和31.31%,均高于P1（8.18%,21.46%）、P2（6.63%,21.53%）。P1、P2、P3均具有较强的抗氧化能力。其中P3的TBRAS值为0.2495±0.016显著低于P1、P2（p<0.01）,抑制羟自由基能力,Fe2+螯合率和Cu2+螯合率分别为75.75%±0.49%、72.40%±0.80%和69.04%±0.40%,均显著高于P1、P2（p<0.05）;基质辅助激光解吸电离飞行时间质谱鉴定P3的相对分子质量范围在1.02 ku以下。
- 发酵鹅肝肽 /
- 分离纯化 /
- 抗氧化活性 /
- 金属离子螯合活性
Abstract: Fermented peptides from goose liver were prepared using Bacillus amyloliquefaciens,Bacillus licheniformis,and Lactobacillus acidophilus. The fermented peptides were separated and purified by Sephadex G-25 gel filtration chromatography. The composition and content of amino acids were analysed by amino acid analyzer,and then functions( antioxidant activity and metals- chelating activity) of P1,P2,P3 were studied. The relative molecular weight of high- active fraction was determined by MALDI- TOF/ TOF. Three peptide peaks were obtained after Sephadex G- 25 gel filtration chromatography. The content of essential amino acid of P3( 70.32 ±5.35) mg /100 g was significantly higher than P1( 40.06 ± 2.04) mg /100 g,and P2( 55.39 ± 2.56) mg /100 g.Proportions of sulfur- containing amino acids( Met,Cys) and hydrophobic amino acids( Val,Ile,Leu,Phe,Ala) in total amino acids of P3( 17.78%,31.31%) were higher than those of P1( 8.18%,21.46%) and P2( 6.63%,21.53%).P1,P2 and P3 all possessed strong antioxidant activity. The TBRAS of P3( 0.2495 ± 0.016) was significantly smaller than P1,P2( p < 0.01),the hydroxyl radical scavenging ratio 75.75% ± 0.49%,Fe2 +chelating activity 72.40% ± 0.80%and Cu2 +chelating activity 69.04% ± 0.40% was significantly higher than P1,P2( p < 0.05).The relative molecular weight of P3 was determined to be lower than 1.02 ku by MALDI- TOF/ TOF.
- fermented peptide from goose liver /
- purification /
- antioxidant activity /
- metal chelating activity

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