HPLC法测定姜黄根茎及姜黄素饮料中姜黄素类化合物的含量
High-performance liquid chromatography method for quantitative determination of curcuminoids in rhizomes of Curcuma Longa L.and curcumin beverage
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摘要: 建立高效液相色谱(HPLC)测定姜黄根茎以及姜黄素饮料中姜黄素类化合物的含量。样品用乙醇辅助超声波提取,以DIKMA Platisil ODS(4.6 mm×250 mm,5μm)柱为分析柱,乙腈和水(磷酸调p H为3.0)为流动相进行梯度洗脱,流速:1.0 m L/min,检测波长:425 nm,柱温:30℃。姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素分别在1.00~200.00,0.96~192.00,0.96~192.00 mg/L范围内线性关系良好,相关系数R2>0.999。平均回收率分别为106.87%(RSD=1.24%)、105.28%(RSD=0.48%)、96.86%(RSD=0.26%)。本方法操作简单、快速、重复性好,可用于姜黄根茎及饮料中的姜黄素类化合物的检测。Abstract: A HPLC method was established for the determination of curcuminoids content in rhizomes of Curcuma Longa L. and curcumin beverage.The samples were extracted in ethanol by ultrasonic treatment.The determination by RP-HPLC was carried out using DIKMA Platisil ODS column( 4.6 mm × 250 mm,5 μm) and acetonitrile-water( p H = 3.0 adjusted by phosphoric acid) with gradient elution at a flow rate of 1 m L/min. The UV detection wavelength was 425 nm and the column temperature was set at 30 ℃.The calibration curves were linear in the range of 1.00 ~ 200.00,0.96 ~ 192.00,0.96 ~ 192.00 mg / L( R2> 0.999) for curcumin,demethoxycurcumin and bisdemethoxycurcumin.The average recoveries were 106.87%( RSD = 1.24%),105.28%( RSD = 0.48%) and 96.86%( RSD = 0.26%),respectively. The method was simple,rapid and reproducible,and can be used for the determination of curcuminoids in rhizomes of Curcuma longa L.and curcumin beverage.
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