双酶法提取豆豉多糖的工艺研究

吉义平; 费珊; 丁彩梅

doi:10.13386/j.issn1002-0306.2016.21.041

双酶法提取豆豉多糖的工艺研究

武汉设计工程学院食品与生物科技学院

基金项目:

武汉设计工程学院校科研项目(201302)；

详细信息

作者简介:
吉义平(1984-),女,硕士,讲师,研究方向:食品功能性成分的提取纯化、检测及其应用,E-mail:343677874@qq.com。;

中图分类号: TQ281;TS214
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出版历程
- 收稿日期: 2016-03-31

Research on dual-enzymatic extraction of polysaccharides from Douchi

College of Food and Biology Science and Technology,Wuhan Institute of Design and Sciences

摘要

摘要: 在单因素实验基础上应用正交实验设计法对双酶法提取豆豉多糖的工艺进行了优化。优化后的最佳提取工艺为:首先在料液比1∶20(g∶m L)、p H7.0和温度50℃的条件下,添加2.0%的木瓜蛋白酶进行水解150 min。然后在料液比1∶20(g∶m L)、p H7.0和55℃的条件下继续添加2.5%的风味蛋白酶水解150 min,两次分步酶解后测得多糖得率为15.90%。此优化后的豆豉多糖提取工艺合理、可行且得率较高。
- 豆豉 /
- 多糖 /
- 双酶法
Abstract: Based on the single factor experiments,the orthogonal experimental design was employed to optimize the extraction conditions of polysaccharide from douchi via dual-enzymatic.The results showed that the optimum dual-enzymatic extracting conditions were as follows: firstly,the ratio of water to raw material 1∶ 20( g ∶ m L),p H7.0and extraction temperature 50 ℃,enzyme dosage of papain 2% and hydrolysis time 150 min.Secondly,p H7.0 and extraction temperature 55 ℃,with flavourzyme 2.5%,hydrolysis time 150 min.Under these step by step enzymatic hydrolysis conditions,the extraction yield was 15.90%. The optimization of extraction process technology of polysaccharides from douchi was reasonable and feasible and yield was higher.
- douchi /
- polysaccharides /
- dual-enzymatic

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参考文献(22)

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