Optimization of extraction conditions for hydrocyanic acid in flaxseed by response surface methodology
-
摘要: 为了提高亚麻籽中的氢氰酸含量检测的效率,同时确保其准确性,用响应面法对异烟酸-巴比妥酸比色法前处理中的提取方法进行了优化。运用Design Expert 7.0数据分析软件在单因素实验的基础上采用3因素3水平的响应面分析法,建立了氢氰酸提取率与各影响因子的回归方程。结果表明:氢氰酸的最佳提取条件为液固比50∶1（m L/g）、提取时间45 min、氢氧化钠吸收液浓度10 g/L,氢氰酸实际提取率可达96.12%。结论:此方法真实可靠,可用于大批量检测亚麻籽中的氢氰酸含量。
-
关键词:
- 亚麻籽 /
- 氢氰酸 /
- 生氰糖苷 /
- 异烟酸-巴比妥酸比色法 /
- 响应面法
Abstract: In order to improve the efficiency of detection for hydrocyanic acid content in flaxseed,while ensuring its accuracy,The pretreatment method of isonicotinic-barbituric acid colorimetric method had been optimized by response surface methodology.A quadratic polynomial regression model was fitted using a three-factor,three-level Box-Behnken design and analyzed using response surface methodology to obtain the optimal extraction conditions.The result showed that the best extraction technology conditions was at the ratio of solvent and material50∶ 1,with 10 g / L concentration of sodium hydroxide absorbing liquid and extracting for 45 min.The hydrocyanic acid extraction ratio could reach 96.12%.This method is true and reliable,can be used to detect large quantities of hydrocyanic acid in flaxseed. -
[1] 孙兰萍,许晖.亚麻籽生氰糖苷的研究进展[J].中国油脂,2007,32(10):24-26. [2] 林非凡,谭竹钧.亚麻籽油中α-亚麻酸降血脂功能研究[J].中国油脂,2012,37(9):44-47. [3] 林凤英,林志光,邱国亮,等.亚麻籽的功能成分及应用研究进展[J].食品工业,2014,35(2):220-223. [4] 张静雯.膳食纤维的功能及在食品中的应用[J].食品工程,2011,(4):17-18. [5] Abuelgassim A O.Effect of flaxseeds and date palm leaves extracts on serum concentrations of glucose and lipids in alloxan diabetic rats[J].Pakistan Journal of Biological Sciences,2010,13,1141-1145.
[6] Makni M,Sefi M,Garouiel M,et al.Dietary polyunsaturated fatty acid prevents hyperlipidemia and hepatic oxidant status in pregnant diabetic rats and their macrosomic offspring[J].Journal of Diabetes Complications,2011,25,267-274.
[7] Sturgeon SR,Volpe SL,Puleo E,et al.Dietary Intervention of Flaxseed,Effect on Serum Levels of IGF-1,IGF-BP3,and C-Peptide.Nutrition and Cancer,2011,63,376-380.
[8] 周小洁,郭亮,车向荣,等.亚麻籽及其饼粕中氰化物测定方法的比较[J].动物生产,2008,44(7):49-51. [9] 李可.亚麻籽粕膳食纤维的提取工艺研究[D].乌鲁木齐:新疆农业大学,2013. [10] 胡晓军,李群,许光映,等.亚麻籽中主要营养成分的分布研究[J].中国油脂.2012,37(12):64-66. [11] 刘义军,魏晓奕,王飞,等.含氰糖苷类作物脱毒技术及其检测方法的研究进展[J].食品工业科技,2013,34(12):357-360. [12] 王志玲,赵忠,朱海兰.异烟酸-巴比妥酸分光光度法测定苦杏仁油氰化物含量的不确定度评定[J].西北农业学报,2010,19(10):174-177. [13] 李高阳,丁霄霖.亚麻籽中氰化物定性定量方法的研究[J].食品工业科技,2008,29(6),291-296. [14] 谭建新,戈京国,哀绰斌,等.异烟酸一巴比妥酸分光光度法(试行)[J].中国环境监测,1994,10(1):13-14. [15] GB/T 15665-1995,豆类配糖氢氰酸含量的测定[S]. [16] 孟凡信,张鸿军,张翠珍.新方法测定杏仁油中氰化物含量[J].监督与选择,2008,3:52-53. [17] 龙云飞,王沛佩,杨克迪,等.响应面法优化亚麻籽油提取工艺[J].食品科学,2012,33(14):45-49. [18] 徐美蓉,李玉芳,董煚,等.大豆异黄酮超声波辅助提取条件优化的研究[J].大豆科学,2013,32(1):52-53.
计量
- 文章访问数: 116
- HTML全文浏览量: 9
- PDF下载量: 142
下载:
