豆腐柴叶总黄酮的提取、纯化及抗氧化活性研究

胡予; 李旭升; 马楠; 张学敏; 毛御波; 陈黎萍; 熊双丽; 薛朝贵

doi:10.13386/j.issn1002-0306.2016.20.045

豆腐柴叶总黄酮的提取、纯化及抗氧化活性研究

西南科技大学生命科学与工程学院
四川省生物质资源利用与改性工程技术研究中心
江油市春雨生态农业科技有限公司

基金项目:

四川省生物质资源利用与改性工程技术研究中心专职科研创新团队建设基金项目(14tdgc04)；

详细信息

作者简介:
胡予(1995-),女,大学本科,研究方向:食品生物化学,E-mail:643844210@qq.com。;

熊双丽(1977-),女,教授,研究方向:功能性食品加工与安全,E-mail:372364129@qq.com。;

中图分类号: R284
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出版历程
- 收稿日期: 2016-03-22

Extraction,purification and antioxidant activity of total flavonoids from Premna microphylla Turcz leaves

School of Life Science and Engineering,Southwest University of Science and Technology
Engineering Research Center for Biomass Resource Utilization and Modification of Sichuan Province
Jiangyou Chunyu Ecological Agricultural Science and Technology Company

摘要

摘要: 在单因素实验基础上,采用响应面法优化豆腐柴叶总黄酮提取工艺,并筛选适合于纯化总黄酮的大孔吸附树脂,最后进行初步鉴定和自由基清除活性分析。结果表明:乙醇浸提总黄酮最佳工艺条件为:乙醇浓度84%、料液比1∶37（g/m L）、温度81℃、时间3.4 h,该条件下总黄酮得率为7.29%。X-5型大孔吸附树脂适用于提取豆腐柴叶总黄酮。紫外-可见光谱分析和显色反应初步鉴定其可能为二氢黄酮。豆腐柴叶总黄酮DPPH自由基和羟基自由基的清除率随浓度的增加而增大,半数抑制浓度值分别为0.10 mg/m L和0.13 mg/m L。当浓度为0.2 mg/m L和0.4 mg/m L时,DPPH自由基和羟基自由基清除率分别为83.75%和80.08%,均接近于叔丁基羟基茴香醚。因此,豆腐柴总黄酮具有较好的抗氧活性,可作为抗氧化剂或者健康食品原料开发。
- 豆腐柴 /
- 总黄酮 /
- 提取工艺 /
- 优化 /
- 抗氧化活性
Abstract: On the basis of the single factor experiment,this article firstly adopted response surface methodology to optimize the extraction technology of total flavonoids from Premna microphylla Turcz leaves,then screened macroporous adsorptive resins which were suitable for extracting total flavonoids,at last made the preliminary identification and analyzed free radical scavenging activity of them.The results showed that the optimal conditions of the ethanol extraction were as follows: ethanol concentration for 84%,solid- liquid ratio for 1 ∶ 37( g / m L),temperature for 81 ℃ and time for 3.4 h. Under these conditions,we could get 7.29% of the total flavonoids.Meanwhile,the macroporous adsorptive resin X- 5 was suitable for the separation of total flavonoids. The colour reaction and UV- vis spectrum exhibited that dihydroflavone was probably the major components. The DPPH radical and hydroxyl radical scavenging rate of the premna leaves flavonoids increased with the increasing of the concentration and the IC50 values were 0.10 mg / m L and 0.13 mg / m L,respectively. When the concentrations were0.2 mg / m L and 0.4 mg / m L. The DPPH radical and hydroxyl radical scavenging rate were 83.75% and 80.08%,respectively,which were close to that of tert- butyl hydroxy anisole. These data suggested that flavonoids from premna microphylla turcz leaves were developed as antioxidant or raw materials of health food.
- Premna microphylla Turcz /
- total flavonoids /
- extraction technology /
- optimize /
- antioxidant activity

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参考文献(19)

[1]	宁海凤,童群义.混合酸提取豆腐柴叶中果胶的研究[J].食品工业科技,2011,32(1):222-228.
[2]	楚文靖,王世强,谢可为.豆腐柴的开发及利用现状[J].食品研究与开发,2011,32(7):175-177.
[3]	Santos BL,Oliveira MN,Coelho PLC,et al.Flavonoids suppress human glioblastoma cell growth by inhibiting cell metabolism,migration,and by regulating extracellular matrix proteins and metalloproteinases expression Original[J].ChemicoBiological Interactions,2015,242(5):123-138.
[4]	曹稳根,焦庆才.豆腐柴根提取物抗炎作用的实验研究[J].中国中医药科技,2002,9(4):223-224.
[5]	Li JE,Fan ST,Qiu ZH,et al.Total flavonoids content,antioxidant and antimicrobial activities of extracts from Mosla chinensis Maxim.cv.Jiangxiangru[J].LWT-Food Science and Technology,2015,64(2):1022-1024.
[6]	罗文谦,王琳,鲁绪会,等.野生豆腐柴总黄酮及微量元素测定[J].安徽农业科学,2009,37(14):6429-6430.
[7]	曹稳根,段红,翟科峰,等.正交设计优选豆腐柴叶总黄酮的微波提取工艺[J].光谱实验室,2013,30(5):2174-2178.
[8]	林颖华,吴晓玲,彭荣珍,等.响应面法优化豆腐柴中总黄酮提取工艺[J].中国医药报,2013,10(18):116-118.
[9]	王军,王敏,季璐.苦荞麦麸皮总黄酮提取工艺及其数学模型研究[J].农业工程学报,2006,22(7):223-225.
[10]	Zhao P,Qi C,Wang G,et al.Enrichment and purification of total flavonoids from Cortex Juglandis Mandshuricae extracts and their suppressive effect on carbon tetrachloride-induced hepatic injury in Mice[J].Journal of Chromatography B,2015,1007(15):8-17.
[11]	王俊,赵辉,吴福安,等.树脂吸附法分离纯化桑叶总黄酮-(I)H103树脂对桑叶水提取液中总黄酮的吸附性能[J].离子吸附与交换,2008,24(2):139-147.
[12]	徐怀德.天然产物提取工艺学[M].北京:中国轻工业出版社,2006:346-348.
[13]	Jabbari M,Jabbari A.Antioxidant potential and DPPH radical scavenging kinetics of water-insoluble flavonoid naringenin in aqueous solution of micelles[J].Colloids and Surfaces A:Physicochemical and Engineering Aspects,2016,489(20):392-399.
[14]	Herraiz T,Galisteo J.Hydroxyl radical reactions and the radical scavenging activity ofβ-carboline alkaloids[J].Food Chemistry,2015,172(1):640-649.
[15]	孙协军,李秀霞,冯彦博,等.华山楂黄酮超高压提取工艺研究[J].食品工业科技,2015,36(2):291-295.
[16]	Zhao ZY,Dong LL,Wu YL,et al.Preliminary separation and purification of rutin and quercetin from Euonymus alatus(Thunb.)Siebold extracts by macroporous resins[J].Food and Bioproducts Processing,2011,89(4):266-272.
[17]	Guo HD,Zhang QF,Chen JG,et al.Large scale purification of puerarin from Puerariae Lobatae Radix through resins adsorption and acid hydrolysis[J].Journal of Chromatography B,2015,980(1):8-15.
[18]	Pavithra K,Vadivukkarasi S.Evaluation of free radical scavenging activity of various extracts of leaves from Kedrostis foetidissima(Jacq.)Cogn[J].Food Science and Human Wellness,2015,4(1):42-46.
[19]	赵谋明,刘敏,林恋竹,等.山苦茶多糖结构表征及抗氧化活性研究[J].现代食品科技,2015,31(7):61-66.

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