豆腐柴叶总黄酮的提取、纯化及抗氧化活性研究

胡予; 李旭升; 马楠; 张学敏; 毛御波; 陈黎萍; 熊双丽; 薛朝贵

doi:10.13386/j.issn1002-0306.2016.20.045

豆腐柴叶总黄酮的提取、纯化及抗氧化活性研究

西南科技大学生命科学与工程学院
四川省生物质资源利用与改性工程技术研究中心
江油市春雨生态农业科技有限公司

基金项目:

四川省生物质资源利用与改性工程技术研究中心专职科研创新团队建设基金项目(14tdgc04)；

详细信息

作者简介:
胡予(1995-),女,大学本科,研究方向:食品生物化学,E-mail:643844210@qq.com。;

熊双丽(1977-),女,教授,研究方向:功能性食品加工与安全,E-mail:372364129@qq.com。;

中图分类号: R284
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出版历程
- 收稿日期: 2016-03-22

Extraction,purification and antioxidant activity of total flavonoids from Premna microphylla Turcz leaves

School of Life Science and Engineering,Southwest University of Science and Technology
Engineering Research Center for Biomass Resource Utilization and Modification of Sichuan Province
Jiangyou Chunyu Ecological Agricultural Science and Technology Company

摘要

摘要: 在单因素实验基础上,采用响应面法优化豆腐柴叶总黄酮提取工艺,并筛选适合于纯化总黄酮的大孔吸附树脂,最后进行初步鉴定和自由基清除活性分析。结果表明:乙醇浸提总黄酮最佳工艺条件为:乙醇浓度84%、料液比1∶37（g/m L）、温度81℃、时间3.4 h,该条件下总黄酮得率为7.29%。X-5型大孔吸附树脂适用于提取豆腐柴叶总黄酮。紫外-可见光谱分析和显色反应初步鉴定其可能为二氢黄酮。豆腐柴叶总黄酮DPPH自由基和羟基自由基的清除率随浓度的增加而增大,半数抑制浓度值分别为0.10 mg/m L和0.13 mg/m L。当浓度为0.2 mg/m L和0.4 mg/m L时,DPPH自由基和羟基自由基清除率分别为83.75%和80.08%,均接近于叔丁基羟基茴香醚。因此,豆腐柴总黄酮具有较好的抗氧活性,可作为抗氧化剂或者健康食品原料开发。
- 豆腐柴 /
- 总黄酮 /
- 提取工艺 /
- 优化 /
- 抗氧化活性
Abstract: On the basis of the single factor experiment,this article firstly adopted response surface methodology to optimize the extraction technology of total flavonoids from Premna microphylla Turcz leaves,then screened macroporous adsorptive resins which were suitable for extracting total flavonoids,at last made the preliminary identification and analyzed free radical scavenging activity of them.The results showed that the optimal conditions of the ethanol extraction were as follows: ethanol concentration for 84%,solid- liquid ratio for 1 ∶ 37( g / m L),temperature for 81 ℃ and time for 3.4 h. Under these conditions,we could get 7.29% of the total flavonoids.Meanwhile,the macroporous adsorptive resin X- 5 was suitable for the separation of total flavonoids. The colour reaction and UV- vis spectrum exhibited that dihydroflavone was probably the major components. The DPPH radical and hydroxyl radical scavenging rate of the premna leaves flavonoids increased with the increasing of the concentration and the IC50 values were 0.10 mg / m L and 0.13 mg / m L,respectively. When the concentrations were0.2 mg / m L and 0.4 mg / m L. The DPPH radical and hydroxyl radical scavenging rate were 83.75% and 80.08%,respectively,which were close to that of tert- butyl hydroxy anisole. These data suggested that flavonoids from premna microphylla turcz leaves were developed as antioxidant or raw materials of health food.
- Premna microphylla Turcz /
- total flavonoids /
- extraction technology /
- optimize /
- antioxidant activity

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