Abstract: Muscat Grape wes used as the material to extract stilbenes through ultrasonic method.Combined with the single factor test results,response surface methodology( RSM) was applied to optimize the extraction conditions.And the total content of Resveratrol,Piceid and Pterostilbene was used as the investigation index.A maximum extraction rate was achieved.The optimal extraction conditions were used Ethyl acetate as solvent,temperature of57 ℃,extraction time of 32 min,and solid to liquid ratio 1 ∶ 15 g·m L- 1. Under the optimal extraction conditions,the predicted value of stilbenes in Muscat Grape was 105.30 μg / g,and the actual value was 104.97 μg / g.Moreover,the relative error of the established model for extraction efficiency was 0.31% compared with the predicted value.The maximum extraction rate was appropriate for the extraction of stilbenes in Muscat Grape.