响应面法优化金花葵多糖提取工艺

Response surface methodology for optimizing extraction of polysaccharides from Aurea HELIANTHUS

摘要: 以金花葵的茎叶为原料,用水提法对其多糖成分进行提取,研究不同提取条件对金花葵多糖得率的影响。本研究以金花葵多糖的得率为考察指标,对提取温度、提取时间、料液比进行单因素实验的基础上,再利用响应面法优化金花葵多糖的提取条件。结果表明,金花葵多糖的最佳提取工艺条件为提取温度72℃、提取时间3.25 h、料液比1∶32(g∶m L)。金花葵多糖得率的验证值,与预测值的相对误差为0.23%,说明采用响应面法对金花葵多糖提取条件进行优化可行合理。

Abstract: In Aurea HELIANTHUS stems and leaves as raw material,its water extracted polysaccharide formulation to study the effect of different extraction conditions on the yield of polysaccharides.In this study,the polysaccharide yield was investigated indicators.Using response surface optimization of extraction conditions of Aurea HELIANTHUS which basing on the extraction temperature,extraction time,solid- liquid ratio of single factor experiments.Results indicated that the optimum conditions for polysaccharide from Aurea HELIANTHUS extraction were: water temperature of 72 ℃,an extraction time of 3.25 h and raw materials to a ratio of water of 1∶ 32( g∶ m L).Relative error verification and predicted values of polysaccharide yield was 0.23%. Description Response surface methodology for Aurea HELIANTHUS extraction conditions were optimized reasonably feasible.