响应面法优化南海鸢乌贼墨汁多糖提取工艺

杨丽芝; 杨贤庆; 黄卉; 李来好; 邓建朝; 赵永强; 杨少玲

doi:10.13386/j.issn1002-0306.2016.18.047

响应面法优化南海鸢乌贼墨汁多糖提取工艺

1. 中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室,国家水产品加工技术研发中心
2. 上海海洋大学食品学院

基金项目:

农业部财政重大专项(NFZX2013)；南海渔业资源调查与评估；

详细信息

作者简介:
杨丽芝(1989-),女,硕士,研究方向:水产品加工与质量安全,E-mail:yanglizhi926@126.com。;

杨贤庆(1963-),男,研究员,研究方向:水产品加工与质量安全,E-mail:yxqgd@163.com。;

中图分类号: TQ281
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出版历程
- 收稿日期: 2016-01-17

Optimization of extraction technology polysaccharide from the ink of the Sthenoteuthis oualaniensis in South China Sea by response surface methodology

Key Laboratory of Aquatic Product Processing,Ministry of Agriculture,National R & D Center for Aquatic Product Processing,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Science
College of Food Science and Technology,Shanghai Ocean University

摘要

摘要: 目的:为探索南海鸢乌贼墨汁多糖的最佳提取工艺,利用响应面分析法对南海鸢乌贼墨汁多糖提取工艺进行优化。方法:在单因素实验基础上选取因素与水平,根据中心组合实验设计原理采用三因素三水平的响应面分析法,以获得多元二次线性回归方程,以Y(多糖含量(%)×粗多糖得率(%)×104)为响应值分析各因素的交互作用。结果:鸢乌贼墨汁多糖提取的最佳工艺条件:料液比1∶2、浸提液p H5.5、加酶量1.00%,此条件下,粗多糖得率为0.91%,多糖含量为9.8%,与理论预测值接近。结论:采用响应面法优化鸢乌贼墨多糖提取工艺,取得最佳提取工艺,且具有可行性。
- 鸢乌贼 /
- 墨汁多糖 /
- 提取 /
- 响应面分析
Abstract: Objective: To explore the optimum extraction process of polysaccharide from the ink of the Sthenoteuthis oualaniensis in South China Sea,response surface methodology( RSM) was employed to optimize extraction process of polysaccharide from the ink of the Sthenoteuthis oualaniensis. Methods: Experiment factors and levels were first selected on the ground of single factor experiment.Along with the center combination experiment design principle,three factors and three levels of response surface analysis were adopted to obtain a multiple quadratic linear regression equation and analyzed the interaction among factors chosen Y( polysaccharide content × total sugar quality,100 g ink extraction product of quality of crude polysaccharide and total sugar content%) as the response value. Results: The optimum extraction conditions of polysaccharide from the ink of the Sthenoteuthis oualaniensis in South China Sea were concluded as follows: ratio of solid to liquid 1∶ 2,water extract p H5.5,enzyme concentration 1.00%.Under this condition,polysaccharide content and crude polysaccharide extraction yield value of polysaccharide from the ink of the Sthenoteuthis oualaniensis was 9.8% and 0.91% respectively.Conclusion: The RSM was employed to optimize extraction process of polysaccharide from the ink,which had feasibility and good effect.
- Sthenoteuthis oualaniensis /
- ink polysaccharide /
- extraction /
- response surface methodology

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