Optimization of extraction technology polysaccharide from the ink of the Sthenoteuthis oualaniensis in South China Sea by response surface methodology
-
摘要: 目的:为探索南海鸢乌贼墨汁多糖的最佳提取工艺,利用响应面分析法对南海鸢乌贼墨汁多糖提取工艺进行优化。方法:在单因素实验基础上选取因素与水平,根据中心组合实验设计原理采用三因素三水平的响应面分析法,以获得多元二次线性回归方程,以Y(多糖含量(%)×粗多糖得率(%)×104)为响应值分析各因素的交互作用。结果:鸢乌贼墨汁多糖提取的最佳工艺条件:料液比1∶2、浸提液p H5.5、加酶量1.00%,此条件下,粗多糖得率为0.91%,多糖含量为9.8%,与理论预测值接近。结论:采用响应面法优化鸢乌贼墨多糖提取工艺,取得最佳提取工艺,且具有可行性。Abstract: Objective: To explore the optimum extraction process of polysaccharide from the ink of the Sthenoteuthis oualaniensis in South China Sea,response surface methodology( RSM) was employed to optimize extraction process of polysaccharide from the ink of the Sthenoteuthis oualaniensis. Methods: Experiment factors and levels were first selected on the ground of single factor experiment.Along with the center combination experiment design principle,three factors and three levels of response surface analysis were adopted to obtain a multiple quadratic linear regression equation and analyzed the interaction among factors chosen Y( polysaccharide content × total sugar quality,100 g ink extraction product of quality of crude polysaccharide and total sugar content%) as the response value. Results: The optimum extraction conditions of polysaccharide from the ink of the Sthenoteuthis oualaniensis in South China Sea were concluded as follows: ratio of solid to liquid 1∶ 2,water extract p H5.5,enzyme concentration 1.00%.Under this condition,polysaccharide content and crude polysaccharide extraction yield value of polysaccharide from the ink of the Sthenoteuthis oualaniensis was 9.8% and 0.91% respectively.Conclusion: The RSM was employed to optimize extraction process of polysaccharide from the ink,which had feasibility and good effect.
-
[1] 张鹏,杨吝,张旭丰,等.南海金枪鱼和鸢乌贼资源开发现状及前景[J].南方水产,2010,6(1):68-74. [2] 余为,陈新军.印度洋西北海域鸢乌贼9—10月栖息地适宜指数研究[J].广东海洋大学学报,2013,32(6):74-80. [3] 张俊,陈作志,陈国宝,等.南海鸢乌贼水声学测量和评估相关技术研究[J].南方水产科学,2014,10(6):1-11. [4] 冯波,颜云榕,张宇美,等.南海鸢乌贼(Sthenoteuthis oualaniensis)资源评估的新方法[J].渔业科学进展,2014,35(4):1-6. [5] 吕昌龙,洪明标,钟建国,等.乌贼墨对小鼠移植瘤的抑制作用[J].实用肿瘤学杂志,1994,8(1):6-7. [6] 黄枚.乌贼墨抗癌活性的实验研究[J].福建中医学院学报,2004,14(1):23-24. [7] 苏伟明,马润娣,于立坚,等.中国枪乌贼墨汁提取物的抗肿瘤作用[J].中国海洋药物,2005,24(2):47-50. [8] Takaya Y,Uchisawa H,Narumi F,et al.Illexins A,B,and C from squid ink should have a branched structure[J].Biochemical and Biophysical Research Communications,1996,226(2):335-338.
[9] 谢光临,吕昌龙,洪明标.乌贼墨促凝血作用机制的实验研究[J].中国医科大学学报,1994,23(6):530-530. [10] 景奕文,曾丽,王加斌,等.海洋头足类活性物质的功能特性研究进展[J].浙江海洋学院学报:自然科学版,2013,32(5):457-461. [11] 谢明勇,聂少平.天然产物活性多糖结构与功能研究进展[J].中国食品学报,2010(2):1-11. [12] Vate N K,Benjakul S.Antioxidative activity of melanin-free ink from splendid squid(Loligo formosana)[J].International Aquatic Research,2013,5(1):9.
[13] Wang S,Cheng Y,Wang F,et al.Inhibition activity of sulfated polysaccharide of Sepiella maindroni ink on matrix metalloproteinase(MMP)-2[J].Biomedicine&Pharmacotherapy,2008,62(5):297-302.
[14] 乐小炎,原林,林少杰,等.乌贼墨多糖的制备及对睾丸化疗性损伤的保护作用[J].食品工业科技,2013,34(17):342-344. [15] 吴金龙,刘华忠,师莉莎,等.乌贼墨多糖提取工艺优化[J].食品工业科技,2011(7):289-291. [16] 李瑞.翡翠贻贝糖胺聚糖抗肿瘤活性及其免疫调节作用的研究[D].湛江:广东海洋大学,2010. [17] S Sarna T,Sealy R C.Photoinduced oxygen consumption in melanin systems.Action spectra and quantum yields for eumelanin and synthetic melanin[J].Photochemistry and photobiology,1984,39(1):69-74.
[18] 杨勇杰,姜瑞芝,陈英红,等.苯酚-硫酸法测定杂多糖含量的研究[J].中成药,2005,27(6):706-708. [19] 曾少葵,刘坤,吴艺堂,等.脱钙罗非鱼鱼鳞明胶提取工艺优化及其理化性质[J].南方水产科学,2013,9(2):38-44. [20] 王娜.费菜多糖的提取及分离纯化研究[D].泉州:华侨大学,2013. [21] 王忠良,黄建盛,张健东,等.响应面法分析光照强度与盐度对卤虫休眠卵孵化率的联合效应[J].南方水产科学,2014,10(3):80-85. [22] 戚勃,李来好,杨贤庆,等.响应曲面法优化麒麟菜卡拉胶碱处理工艺[J].南方水产科学,2011,7(6):26-34. [23] Xu H,Sun L P,Shi Y Z,et al.Optimization of cultivation conditions for extracellular polysaccharide and mycelium biomass by Morchella esculenta As51620[J].Biochemical Engineering Journal,2008,39(1):66-73.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
