Study on ultrasonic- assisted solvent extraction and purification of pepsin- soluble collagen from grass carp skin
-
摘要: 本文研究了超声波辅助提取草鱼皮胶原蛋白的工艺。以超声功率、超声时间和酶解时间为实验因素,胶原蛋白提取率为响应值,利用Box-Behnken设计实验及响应面法分析实验,确定其最佳提取工艺条件。结果显示:最佳工艺条件为超声功率310 W,超声时间10 min,酶解时间29 h,此时胶原蛋白提取率达到68.67%,与模型理论预测值（68.80%）较为相近,相对误差仅为0.189%。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳图谱（SDS-PAGE）和傅里叶红外光谱（FTIR）谱图,对比超声波作用下与无超声波作用的胶原蛋白发现,超声波对胶原蛋白结构无明显影响。Abstract: Ultrasonic- assisted solvent extraction and purification pepsin- soluble collagen from grass carp skin was investigated in this paper.Ultrasonic rate,ultrasonic time and enzyme extraction time were chosen as influencing factors.The extraction rate of collagen was selected as response value.The mathematical model was established by Box- Behnken central composite design.The analysis method was response surface methodology( RSM).The final optimum conditions were: ultrasonic rate 310 W,ultrasonic time 10 min and enzyme extraction time 29 h,extraction rate of collagen was 68.67%,it's nearly to the model 68.8%,relative tolerance was 0.189%.SDS- PAGE and FTIR showed that ultrasonic- assisted extraction hadno obvious effect on collagen structure.
-
Keywords:
- ultrasonic-assisted extraction /
- grass carp skin /
- collagen /
- SDS-PAGE /
- FTIR
-
[1] 农业部渔业局编制.中国渔业统计年鉴[M].中国农业出版社,2015. [2] 熊善柏.湖北省淡水鱼加工业现状、问题与发展方向[J].科学养鱼,2014(9):14-16. [3] 王艳.草鱼皮胶原蛋白的提取、性质研究及膜性能表征[D].天津:天津科技大学,2013:26-28. [4] 王希,钟耀广,市川寿.响应面优化超声波快速提取鳕鱼皮中胶原蛋白的工艺研究[J].东北农业大学学报,2014,45(7):117-123. [5] 李莉,顾赛麒,王锡昌,等.响应面法优化酶法提取大鲵皮胶原蛋白工艺[J].中国水产科学,2013,20(4):876-883. [6] 王林,梁秋芳,王振斌,等.深海红鱼胶原蛋白的超声波辅助提取及其理化特性[J].食品研究与开发,2014,35(9):18-22. [7] 张玲,芮汉明.超声波介入鲜猪皮的脱脂工艺研究[J].现代食品科技,2006,22(3):99-101. [8] 杨萌萌,郭兆斌,余群力,等.超声波辅助法提取胶原蛋白工艺研究[J].甘肃农业大学学报,2013,48(3):121-126. [9] Woessner JF.The determination of hydroxyproline in tissue and protein samples containing small proportions of this iminoacid[J].Archives of Biochemistry and Biophysics,1961,93:440.
[10] Matmaroh K,Benjakul S,Prodpran T,et al.Characteristics of acid soluble collagen and pepsin soluble collagen from scale of spotted golden goatfish(Parupeneusheptacanthus)[J].Food Chemistry,2011,129:1179-1186.
[11] Nagai,Takeshi,Araki,et al.Collagen of the skin of ocellatepuffer fish(Takifugurubripes)[J].Food Chemistry,2002,78:137-177.
[12] Kimura S,Ohno Y,Miyauchi Y,et al.Fish skin type I collagen:wide distribution of anα3subunit in teleosts[J].Comparative Biochemistry and Physiology,1987,88(1):27-34.
[13] AOAC.Official methods of analysis of the association of official analytical chemists[M].15th ed.Arlington,Washington DC,USA:Association of Official Analytical Chemists,1990.
[14] LI H,LIU B L,GAO L Z,et al.Studies on bullfrog skin collagen[J].Food Chemistry,2004,84(1):65-69.
[15] ZHU B,DONG X,ZHOU D,et al.Physicochemical properties and radical scavenging capacities of pepsin-solubilized collagen from seacucumber Stichopusjaponicus[J].Food Hydrocolloids,2012,28(1):182-188.
[16] Guzzi Plepis AMD,Goissis G,Das-Gupta DK.Dielectric and pyroelectric characterization of anionic and nativecollagen[J].Polymer Engineering and Science,1996,36(24):2932-2938.
计量
- 文章访问数: 208
- HTML全文浏览量: 40
- PDF下载量: 315
下载:
