Effect of culture medium optimization on two methods of nisin detection
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摘要: 为了提高琼脂扩散法和分光光度法测定乳链菌肽效价的效率,对检验培养基进行了优化,考察使用优化后的培养基对这两种测定乳链菌肽效价方法的影响。以测定结果的相对误差与相对标准偏差为衡量指标,判断使用优化后的培养基是否有利于以上两种检测方法测定乳链菌肽效价的准确度与精密度的提高。结果表明:培养基优化后,分光光度法测定乳链菌肽效价的准确度和精密度得到了提高;琼脂扩散法使用优化培养基测定乳链菌肽效价的结果相对误差较大,且在乳链菌肽浓度较低时无法形成抑菌圈,而使用基本培养基时,琼脂扩散法测定乳链菌肽效价的灵敏度高且相对偏差小,测定结果的准确度较高,故利用琼脂扩散法测定乳链菌肽效价时应选用基本培养基。Abstract: To improve efficiency of the agar diffusion method and spectrophotometry for nisin detection,the culture medium was optimized. The influence of the optimized medium on two methods of nisin detection was further investigated.The relative error and relative standard deviation was used to evaluate whether the precision and sensitivity of nisin quantification were enhanced after medium optimization.The results showed that culture medium optimization improved the precision and accuracy of nisin quantification by using spectrophotometry. On the contrary,in determining nisin concentration by agar diffusion method,the basic medium obtained the higher sensitivity and smaller relative error compared with the optimized culture medium.In addition,it was difficult to form a clear bacteriostatic circle at the low concentration of nisin with the optimized culture medium. Therefore,basic culture medium was a suitable choice when the agar diffusion method was used to determine nisin concentration.
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Keywords:
- culture medium optimization /
- nisin /
- Titer
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