Abstract: In order to construct a comprehensive evaluation system which can make up for the traditional quality evaluation method,the main quality indexes of cold storage,ice and frozen shrimp were determined by factor analysis method and linear regression method. The results showed that the comprehensive quality of Penaeus vannamei was evaluated by the color parameters b*and texture parameters of the 2 core indicators.The 60 groups were used to establish the new model of the fresh degree of the 96.7% groups,according to the discriminant model,three kinds of low- temperature storage in refrigeration 3 d,ice temperature 8 d,frozen storage 42 d reached the shelf life. Therefore,this research constructs the comprehensive evaluation system can replace the traditional evaluation method to evaluate the comprehensive quality of the Penaeus vannamei during low-temperature storage.