水果中乙醇含量的气相色谱法测定

侯玉茹; 王宝刚; 李文生; 苗飞; 周家华; 常虹

doi:10.13386/j.issn1002-0306.2016.13.050

水果中乙醇含量的气相色谱法测定

北京市农林科学院林业果树研究所

基金项目:

北京市农林科学院科技创新能力建设专项资助项目(KJCX20140205)；公益性行业(农业)科研专项经费资助(201303075；

详细信息

作者简介:
侯玉茹(1985-),女,硕士,研究方向:果品采后贮藏与加工,E-mail:hou_yuru@126.com。;

王宝刚(1979-),男,博士,副研究员,研究方向:果品物流包装及贮运保鲜技术,E-mail:wangbg1979@126.com。;

中图分类号: O657.71;TS255.7
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出版历程
- 收稿日期: 2015-12-15

Determination of ethanol content in fruits by gas chromatography

Institute of Forestry and Pomology Beijing Academy of Agriculture and Forestry Science

摘要

摘要: 以樱桃、草莓、梨、枣四种水果为试材,采用DB-WAX（60 m×0.25 mm×0.25μm）毛细管色谱柱和氢火焰离子化检测器建立了气相色谱法测定水果中乙醇含量的方法。结果表明,在进样口温度230℃,FID检测器温度300℃,程序升温40℃（保持11 min）,以20℃/min升至220℃（保持13 min）,以氮气为载气,柱流量2 m L/min的条件下,乙醇有良好的分离效果。乙醇含量在0.25¹00μg/m L范围内,具有良好的线性关系（R2=0.99998）,最低检出浓度为1.25 mg/kg。该方法稳定性、重复性和重现性良好,RSD均小于2%。在四种水果低中高三个添加水平下,平均回收率在90.88%¹08.80%之间（RSD=0.18%⁷.18%,n=6）。该方法操作简单,准确可靠,符合方法学要求,可用于水果中乙醇含量的检测。
- 乙醇 /
- 水果 /
- 气相色谱
Abstract: The ethanol content in fruits( cherry,strawberry,pear,jujube) were determined by gas chromatography( GC) equipped with DB-WAX( 60 m × 0.25 mm × 0.25 μm) and hydrogen flame ionization detector.Results showed that the inlet and FID detector temperature were 230 ℃ and 300 ℃,respectively.The column temperature program was initial 40 ℃( held for 11 min),with the increase rate of 20 ℃ / min to 220 ℃( held for 13 min). The carrier gas was nitrogen,and the flow rate was 2 m L / min. The calibration curve showed good linearity when ethanol concentration was in the range of 0.25~100 μg / m L with correlation coefficient of 0.99998,and the detection limit was1.25 mg / kg.The method was stable,repeatable and reproducible with RSD less than 2%. The average recoveries ranged from 90.88% to 108.80%( RSD = 0.18% ~ 7.18%,n = 6). The method was simple,accurate,and met the requirements of methodology,which may be used for the determination of the ethanol in fruits.
- ethanol /
- fruit /
- chromatography(GC)

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