Active carbon decolorization of xylonic acid from insitu whole- cell catalysate of diluted sulfuric acid pre- hydrolyzed corn stover solution
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摘要: 研究旨在探讨活性炭对木质纤维水解液全细胞原位催化制取木糖酸产品的脱色精制技术与工艺条件。在单因素实验基础上,采用正交实验,以脱色率（%）和木糖酸回收率（%）为指标,分别用紫外-可见分光光度法和高效阴离子交换色谱测定研究并确定脱色的最优工艺参数。结果表明:粉状活性炭对秸秆稀酸水解液所制取的木糖酸产品液均具有选择性的吸附功能,对色素杂质的吸附脱除率明显高于对木糖酸的吸附率。C3对木糖酸产品的选择性脱色精制效果最佳。C3活性炭添加量对脱色效果的影响最大,其次为时间和温度,最适脱色工艺条件为:活性炭添加量20%、脱色温度60℃、吸附时间40 min,脱色率达到99.74%,木糖酸回收率可保持90.05%。这说明采用C3粉状活性炭对木糖酸进行脱色和产品精制的技术可行,且快速简便。Abstract: The objective of this work was to investigate the refining technology and process conditions of active carbon decolorization of xylonic acid from insitu whole- cell catalysate of diluted sulfuric acid pre- hydrolyzed corn stover solution. Based on the single factor experiment,the orthogonal design was applied to select the optimal conditions for the decolorization of xylonic acid.The decolorization rate and the recovery rate of xylonic acid were used as indexes which were determined by UV- Vis spectrometer and high performance anion exchange chromatography,respectively. The experimental results showed that powdered activated carbon had selective adsorption function for all xylonic acid products from diluted sulfuric acid pre- hydrolyzed corn stover solution,the removal rate of pigment impurities was significantly higher than the adsorption rate of xylonic acid. C3 performed the best in the adsorption of coloring matters among the three kinds of activated carbon products.The amount of C3 was the most significant factor for decolorization,followed by time and temperature. The optimal conditions for decolorization as follow: the added quantity of activated carbon was 20%( w / w),the temperature was 60 ℃ and the time was 40 min.Under these conditions,the decolorization rate was 99.74% and the recovery rate was 90.05%. It indicated that it was a feasible,convenient and quick technology to decolor and refine of xylonic acid with powdered C3.
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