碳源对裂殖壶菌类胡萝卜素积累的影响

刘源; 李蕾蕾; 张涛; 娄菲; 常明; 金青哲; 王兴国

doi:10.13386/j.issn1002-0306.2016.13.021

碳源对裂殖壶菌类胡萝卜素积累的影响

食品科学与技术国家重点实验室食品安全与营养协同创新中心江南大学食品学院

基金项目:

国家自然基金青年基金项目(31401619)；江苏省自然基金青年基金项目(BK20140156)；

详细信息

作者简介:
刘源(1990-),男,硕士研究生,研究方向:微生物油脂,E-mail:liu314yuan@126.com。;

金青哲(1962-),男,博士,教授,主要从事油脂化学方向,E-mail:jqzwuxi@163.com。;

中图分类号: TQ920.1
计量
- 文章访问数: 148
- HTML全文浏览量: 15
- PDF下载量: 170
出版历程
- 收稿日期: 2015-12-15

Influence of carbon source on the accumulation of carotenoid during the Schizochytrium ATCC20888 fermentation

State Key Laboratory of Food Science and Technology & School of Food Science and Technology,Jiangnan University

摘要

摘要: 本文研究了葡萄糖和甘油分别作为单一碳源对裂殖壶菌ATCC20888发酵过程中脂质和类胡萝卜素变化特性的影响,初步讨论了在裂壶藻中类胡萝卜素与脂质代谢之间的关系。结果表明,作为碳源,葡萄糖相比甘油更有利于菌体生长,而甘油更有利于脂质,尤其是DHA的积累。此外,碳源不同,总类胡萝卜素的含量及组成也会因此而不同。当发酵7 d时,以甘油作碳源发酵得到的β-胡萝卜素含量是以葡萄糖作碳源时的3.25倍。而以葡萄糖为碳源发酵得到的番茄红素和虾青素含量分别是甘油发酵1.60倍和2.81倍。并且,在不同碳源发酵条件下,进入脂质反耗阶段后,菌体内PKS途径的脂肪酸比例均与总类胡萝卜素含量呈正相关,因此表明类胡萝卜素对细胞内多不饱和脂肪酸的稳定性发挥着积极作用。
- 裂殖壶菌 /
- 碳源 /
- 类胡萝卜素 /
- 脂代谢
Abstract: The biosynthesis of lipids and carotenoids during the Schizochytrium ATCC20888 fermentation was investigated in this study using glucose and glycerol as single carbon source,separately. The results showed glucose benefited cell growth more than glycerol while glycerol fermentation was better for the accumulation of lipid especially of DHA.Besides,it showed Different content and composition of carotenoids were observed between the different carbon sources.After 7 days fermentation,the content of β- carotene when used glycerol as carbon source was 3.25 folds of that used glucose,while the content of lycopene and astaxanthin of the latter were 0.60 and 1.81 times higher than the former.There was a positive correlation between the carotenoids content and PKS products during the turnover stage with both carbon source,which indicated that carotenoids might play an important role in increasing the stability of the PUFAs in cells.
- Schizochytrium /
- carbon source /
- carotenoids /
- metabolism of fatty acids

HTML全文

参考文献(22)

[1]	Li Q,Chen GQ,Fan K,et al.Screening and characterization of squalene-producing thraustochytrids from Hong Kong mangroves[J].Journal of Agricultural and Food Chemistry,2009,57(10):4267-4272.
[2]	Ren L J,Sun G N,Ji X J,et al.Compositional shift in lipid fractions during lipid accumulation and turnover in Schizochytrium sp[J].Bioresource Technology,2014(157):107-113.
[3]	Yaguchi T,Tanaka S,Yokochi T,et al.Production of high yields of docosahexaenoic acid by Schizochytrium sp.strain SR21[J].Journal of the American Oil Chemists’Society,1997,74(11):1431-1434.
[4]	魏萍,马小琛,任路静,等.裂殖壶菌发酵生产DHA研究进展[J].食品工业科技,2010,31(10):398-401.
[5]	Hu X,Sun J,Yuan Q.Improvedβ-carotene biosynthesis and gene transcription in Blakeslea trispora with arachidonic acid[J].Biotechnology letters,2012,34(11):2107-2111.
[6]	孙协军,李秀霞,励建荣,等.杜氏盐藻β-胡萝卜素提取皂化工艺参数优化[J].中国食品学报,2014,14(11):90-95.
[7]	Yen H W,Chang J T.Growth of oleaginous Rhodotorula glutinis in an internal-loop airlift bioreactor by using lignocellulosic biomass hydrolysate as the carbon source[J].Journal of Bioscience and Bioengineering,2015,119(5):580-584.
[8]	Mata-Gómez L C,Montaez J C,Méndez-Zavala A,et al.Biotechnological production of carotenoids by yeasts:an overview[J].Microbial Cell Factories,2014,13(1):1-12.
[9]	Nanou K,Roukas T.Oxidative stress response of Blakeslea trispora induced by iron ions during carotene production in shake flask culture[J].Applied Biochemistry and Biotechnology,2013,169(8):2281-2289.
[10]	Nanou K,Roukas T,Papadakis E.Oxidative stress and morphological changes in Blakeslea trispora induced by enhanced aeration during carotene production in a bubble column reactor[J].Biochemical Engineering Journal,2011,54(3):172-177.
[11]	Chang G F,Luo Z L,Gu S T,et al.Fatty acid shifts and metabolic activity changes of Schizochytrium sp.S31 cultured on glycerol[J].Bioresource Technology,2013(142):255-260.
[12]	Ratledge C.Fatty acid biosynthesis in microorganisms being used for single cell oil production[J].Biochimie,2004(86):807-815.
[13]	Yokochi T,Honda D,Higashihara T,et al.Optimization of docosahexaenoic acid production by Schizochytrium limacinum SR21[J].Applied Microbiology and Biotechnology,1998,49(1):72-76.
[14]	Chang G F,Gao N,Tian G,et al.Improvement of docosahexaenoic acid production on glycerol by Schizochytrium sp.S31 with constantly high oxygen transfer coefficient[J].Bioresource Technology,2013(142):400-406.
[15]	Li J,Liu R J,Chang G F,et al.A strategy for the highly efficient production of docosahexaenoic acid by Aurantiochytrium limacinum SR21 using glucose and glycerol as the mixed carbon sources[J].Bioresource Technology,2015(177):51-57.
[16]	张娟梅,江贤章,黄建忠.裂殖壶菌Schizochytrium sp.FJU-512细胞油脂的研究[J].福建师范大学学报:自然科学版,2007,3(2):75-80.
[17]	Bhosale P.Environmental and cultural stimulants in the production of carotenoids from microorganisms[J].Appl Microbiol Biotechnol,2004,63(4):351-361.
[18]	Lippmeier J C,Crawford K S,Owen C B,et al.Characterization of both polyunsaturated fatty acid biosynthetic pathways in Schizochytrium sp[J].Lipids,2009,44(7):621-630.
[19]	郑琰晶,李赟.类胡萝卜素合成酶基因及海洋微藻合成类胡萝卜素的研究进展[J].海洋湖沼通报,2004(1):88-95
[20]	Zhang Z,Zhang X,Tan T.Lipid and carotenoid production by Rhodotorula glutinis under irradiation/high-temperature and dark/low-temperature cultivation[J].Bioresource Technology,2014,157(4):149-153.
[21]	Nanou K,Roukas T,Papadakis E.Improved production of carotenes from synthetic medium by Blakeslea trispora in a bubble column reactor[J].Biochemical Engineering Journal,2012,67(2):203-207.
[22]	Davoli P,Mierau V,W S R.Carotenoids and fatty acids in red yeasts sporobolomyces roseus and rhodotorula glutinis[J].Applied Biochemistry and Microbiology,2004(40):392-397.