苏籽油在超临界CO<sub>2</sub>流体中溶解度及其抗氧化性能研究

刘阳阳; 赵修华; 祖元刚; 葛云龙; 李媛媛; 吴铭芳

doi:10.13386/j.issn1002-0306.2016.13.015

苏籽油在超临界CO₂流体中溶解度及其抗氧化性能研究

东北林业大学森林植物生态学教育部重点实验室

基金项目:

林业公益性行业科研专项(20140470102)；

详细信息

作者简介:
刘阳阳(1988-),男,硕士研究生,研究方向:植物化学与植物药,E-mail:lyybotanynefu@163.com。;

祖元刚(1953-),男,博士,教授,研究方向:植物资源生态利用,E-mail:yuangangzu@163.com。;

中图分类号: TS225.1
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出版历程
- 收稿日期: 2016-01-07

Solubility evaluation of Perilla frutescens seed oil in supercritical carbon dioxide and its antioxidative activity

Key Laboratory of Forest Plant Ecology,Northeast Forestry University,Ministry of Education

摘要

摘要: 采用超临界CO₂流体萃取技术分别探讨压力、温度对苏籽油溶解度及得率的影响,利用Chrastil方程对不同萃取条件下苏籽油溶解度进行模型拟合,并利用GC-MS技术对超临界CO₂流体萃取和索氏提取得到的苏籽油进行脂肪酸成分分析,并利用DPPH（1,1-二苯基-2-三硝基苯肼）测定苏籽油的自由基清除能力。结果表明,相同温度下,苏籽油溶解度与得率随压力增大而增大;相同压力下,苏籽油溶解度与得率随温度增大而减小;在313 K,35 MPa条件下,溶解度S最大为16.68 g/L,并得到以温度（T）和CO₂密度（ρ）为相关因素的苏籽油溶解度模型方程;苏籽油中不饱和脂肪酸含量达90%以上,且不同提取方法得到的苏籽油组分差异不大,超临界萃取与索氏提取得到的苏籽油的自由基清除能力的IC₅₀值分别为22.66、27.76 mg/m L。
- 苏籽油 /
- 溶解度 /
- DPPH /
- 超临界CO2 /
- Chrastil模型
Abstract: In this research,oil was extracted from Perilla frutescens seed by using supercritical carbon dioxide( SC-CO₂) to investigate the effect of pressure and temperature on the solubility and yield.The solubility data was correlated based on the Chrastil model,fatty acid compositions of Perilla frutescens seed oil was further investigated through gas chromatography- mass spectrometry.Resualts showed that solubility was increased with increasing pressure at constant temperature and decreased with increasing temperature at constant pressure.The highest solubility was obtained at 313 K,35 MPa.A solubility model of Perilla frutescens seed oil based on the temperature and the density of CO₂ was obtained. Morever,GC- MS analysis results showed no significant difference in the fatty acid composition of Perilla frutescens seed oil among different extraction methods and over90% of poly unsaturated fatty acid was obtained from Perilla frutescens seed oil.DPPH- scavenging capacity study showed that the IC₅₀ value of oil extracted using SC- CO₂ and Soxhlet method were 22.66 mg / m L and27.76 mg / m L.
- Perilla frutescens seed oil /
- solubility /
- DPPH· /
- supercritical CO_2 /
- Chrastil model

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